Lessons from the genomes of bifidobacteria

Klijn, Adrianne; Mercenier, Annick; Arigoni, Fabrizio

doi:10.1016/j.femsre.2005.04.010

Cited by 65 publications

(60 citation statements)

References 184 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These inducible stress responses are characterized by the transient induction of genes that encode general and specific stress proteins (e.g., chaperones and ATP-dependent proteases) and corresponding regulatory proteins [3,16,21,32,34,46,47].…”

Section: Introductionmentioning

confidence: 99%

Intrinsic and inducible resistance to hydrogen peroxide in Bifidobacterium species

Oberg

Steele

Ingham

et al. 2011

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Interest in, and use of, bifidobacteria as a probiotic delivered in functional foods has increased dramatically in recent years. As a result of their anaerobic nature, oxidative stress can pose a major challenge to maintaining viability of bifidobacteria during functional food storage. To better understand the oxidative stress response in two industrially important bifidobacteria species, we examined the response of three strains of B. longum and three strains of B. animalis subsp. lactis to hydrogen peroxide (H₂O₂). Each strain was exposed to a range of H₂O₂ concentrations (0-10 mM) to evaluate and compare intrinsic resistance to H₂O₂. Next, strains were tested for the presence of an inducible oxidative stress response by exposure to a sublethal H₂O₂ concentration for 20 or 60 min followed by challenge at a lethal H₂O₂ concentration. Results showed B. longum subsp. infantis ATCC 15697 had the highest level of intrinsic H₂O₂ resistance of all strains tested and B. animalis subsp. lactis BL-04 had the highest resistance among B. lactis strains. Inducible H₂O₂ resistance was detected in four strains, B. longum NCC2705, B. longum D2957, B. lactis RH-1, and B. lactis BL-04. Other strains showed either no difference or increased sensitivity to H₂O₂ after induction treatments. These data indicate that intrinsic and inducible resistance to hydrogen peroxide is strain specific in B. longum and B. lactis and suggest that for some strains, sublethal H₂O₂ treatments might help increase cell resistance to oxidative damage during production and storage of probiotic-containing foods.

show abstract

Section: Introductionmentioning

confidence: 99%

Intrinsic and inducible resistance to hydrogen peroxide in Bifidobacterium species

Oberg

Steele

Ingham

et al. 2011

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Interestingly, in this bacterium many of the genes involved in sugar utilization appear to be clustered in a specific ''life style adaptation'' region in its chromosome, which seems to display a high degree of variability in different strains of this species (Klijn et al 2005). This region includes a-mannosidases and endo-b-NAc-glucosaminidase, that might well contribute to the ecological fitness of NCC2705 strain in the GIT (Klijn et al 2005) by increasing the metabolic capacity of this strain with regards to complex carbohydrates.…”

Section: Carbohydrate Utilizationmentioning

confidence: 99%

Genomics as a means to understand bacterial phylogeny and ecological adaptation: the case of bifidobacteria

Ventura

Canchaya

Fitzgerald

et al. 2006

Antonie van Leeuwenhoek

100

View full text Add to dashboard Cite

The field of microbiology has in recent years been transformed by the ever increasing number of publicly available whole-genome sequences. This sequence information has significantly enhanced our understanding of the physiology, genetics and evolutionary development of bacteria. Among the latter group of microorganisms, bifidobacteria represent important human commensals because of their perceived contribution to maintaining a balanced gastrointestinal tract microbiota. In recent years bifidobacteria have drawn much scientific attention because of their use as live bacteria in numerous food preparations with various health-related claims. For this reason, these bacteria constitute a growing area of interest with respect to genomics, molecular biology and genetics. Recent genome sequencing of a number of bifidobacterial species has allowed access to the complete genetic make-up of these bacteria. In this review we will discuss how genomic data has allowed us to understand bifidobacterial evolution, while also revealing genetic functions that explains their presence in the particular ecological environment of the gastrointestinal tract.

show abstract

“…B. adolescentis are the natural inhabitants in healthy humans since birth to late adulthood. The isolated species has been used to treat infant diarrhea when discovered in 1899, and its presence in the gut has been associated with a healthy microbiota [5]. More importantly, growing body of evidence has revealed more physiological functions of the species, such as relieving liver damage, preventing initiation of colon cancer, and stimulating host's immune response [6].…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests

Chiu

Nam²,

Tsai³

et al. 2013

The Scientific World Journal

View full text Add to dashboard Cite

Genotoxicity assessment is carried out on freeze dried powder of cultured probiotics containing Lactobacillus rhamnosus LCR177, Bifidobacterium adolescentis BA286, and Pediococcus acidilactici PA318. Ames tests, in vitro mammalian chromosome aberration assay, and micronucleus tests in mouse peripheral blood are performed. For 5 strains of Salmonella Typhimurium, the Ames tests show no increased reverse mutation upon exposure to the test substance. In CHO cells, the frequency of chromosome aberration does not increase in responding to the treatment of probiotics. Likewise, the frequency of micronucleated reticulocytes in probiotics-fed mice is indistinguishable from that in the negative control group. Taken together, the toxicity assessment studies suggest that the multispecies probiotic mixture does not have mutagenic effects on various organisms.

show abstract

Lessons from the genomes of bifidobacteria

Cited by 65 publications

References 184 publications

Intrinsic and inducible resistance to hydrogen peroxide in Bifidobacterium species

Intrinsic and inducible resistance to hydrogen peroxide in Bifidobacterium species

Genomics as a means to understand bacterial phylogeny and ecological adaptation: the case of bifidobacteria

Genotoxicity Assessment of Multispecies Probiotics Using Reverse Mutation, Mammalian Chromosomal Aberration, and Rodent Micronucleus Tests

Contact Info

Product

Resources

About