Lessons from <i>Drosophila</i> Pointed, an ETS family transcription factor and key nuclear effector of the RTK signaling pathway

Vivekanand, Pavithra

doi:10.1002/dvg.23257

Cited by 20 publications

(9 citation statements)

References 102 publications

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“…Rlb was localized in a small region of chromosome 2R (Hita et al., 1999, 2006) that contains two genes: CG33136 and edl / mae (ETS-domain lacking)/(modulator of the activity of ETS). Interestingly, Edl interacts via its SAM (sterile alpha motif) domain with several ETS transcription factors (Baker et al., 2001) such as the transcriptional activator Pointed (mainly Pointed P2) and the repressor Yan/Aop (Vivekanand, 2018), both involved in the EGF pathway. When activated, the EGF signal triggers the mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) kinase (MEK) known as Rolled.…”

Section: In Search For New Genes and Signaling Pathways Involved In Ementioning

confidence: 99%

“…When activated, the EGF signal triggers the mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) kinase (MEK) known as Rolled. Among many targets, rolled phosphorylates Pointed and Yan, thus modulating their function by changing their SAM domain binding affinity (Vivekanand, 2018). The universal output of the EGF pathway is induction of gene expression, mostly through the Pointed transcriptional activator.…”

Section: In Search For New Genes and Signaling Pathways Involved In Ementioning

confidence: 99%

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Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process

Kim-Jo¹,

Gatti²,

Poirié³

2019

Front. Physiol.

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Host-parasitoid interactions are among the most studied interactions between invertebrates because of their fundamental interest – the evolution of original traits in parasitoids – and applied, parasitoids being widely used in biological control. Immunity, and in particular cellular immunity, is central in these interactions, the host encapsulation response being specific for large foreign bodies such as parasitoid eggs. Although already well studied in this species, recent data on Drosophila melanogaster have unquestionably improved knowledge of invertebrate cellular immunity. At the same time, the venomics of parasitoids has expanded, notably those of Drosophila . Here, we summarize and discuss these advances, with a focus on an emerging “time-dependent” view of interactions outcome at the intra- and interspecific level. We also present issues still in debate and prospects for study. Data on the Drosophila -parasitoid model paves the way to new concepts in insect immunity as well as parasitoid wasp strategies to overcome it.

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Section: In Search For New Genes and Signaling Pathways Involved In Ementioning

confidence: 99%

Section: In Search For New Genes and Signaling Pathways Involved In Ementioning

confidence: 99%

Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process

Kim-Jo¹,

Gatti²,

Poirié³

2019

Front. Physiol.

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“…As a first example, we investigate the ETS family transcription factor Pointed (Pnt), a known regulator of differentiation and proliferation in multiple fly tissues with implications in hemocyte development (Zettervall et al 2004; Dragojlovic-Munther and Martinez-Agosto 2013; Shwartz et al 2013; reviewed in Vivekanand 2018). Pnt transcript is expressed at very low levels in the PSC, MZ1-1, MZ2-1, and MZ2-2 (Figure 4A-B) and its level rises significantly in MZ2-3 (Figure 4B).…”

Section: Resultsmentioning

confidence: 99%

Paths and Pathways that Generate Cell-Type Heterogeneity and Developmental Progression in Hematopoiesis

Girard

Goins

Vuu

et al. 2021

Preprint

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Mechanistic studies of Drosophila lymph gland hematopoiesis are limited by the availability of cell-type specific markers. Using a combination of bulk RNA-Seq of FACS-sorted cells, single cell RNA-Seq and genetic dissection, we identify new blood cell subpopulations along a developmental trajectory with multiple paths to mature cell types. This provides functional insights into key developmental processes and signaling pathways. We highlight metabolism as a driver of development, show that graded Pointed expression allows distinct roles in successive developmental steps, and that mature crystal cells specifically express an alternate isoform of Hypoxia-inducible factor (Hif/Sima). Mechanistically, the Musashi-regulated protein Numb facilitates Sima-dependent non-canonical, while inhibiting canonical, Notch signaling. Broadly, we find that prior to making a fate choice, a progenitor selects between alternative, biologically relevant, transitory states allowing smooth transitions reflective of combinatorial expressions rather than stepwise binary decisions. Increasingly, this view is gaining support in mammalian hematopoiesis.

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“…EGFR signals via the canonical Ras/MAPK to activate the transcription of target genes in a context-dependent manner via the Pointed ETS transcription factor [61]. Its expression during development is broad and therefore is not considered to be the critical aspect regulating its activity [62].…”

Section: Epidermal Growth Factor Receptormentioning

confidence: 99%

Receptor Tyrosine Kinases in Development: Insights from Drosophila

Mele

Johnson

2019

IJMS

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Cell-to-cell communication mediates a plethora of cellular decisions and behaviors that are crucial for the correct and robust development of multicellular organisms. Many of these signals are encoded in secreted hormones or growth factors that bind to and activate cell surface receptors, to transmit the cue intracellularly. One of the major superfamilies of cell surface receptors are the receptor tyrosine kinases (RTKs). For nearly half a century RTKs have been the focus of intensive study due to their ability to alter fundamental aspects of cell biology, such as cell proliferation, growth, and shape, and because of their central importance in diseases such as cancer. Studies in model organisms such a Drosophila melanogaster have proved invaluable for identifying new conserved RTK pathway components, delineating their contributions, and for the discovery of conserved mechanisms that control RTK-signaling events. Here we provide a brief overview of the RTK superfamily and the general mechanisms used in their regulation. We further highlight the functions of several RTKs that govern distinct cell-fate decisions in Drosophila and explore how their activities are developmentally controlled.

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Lessons from Drosophila Pointed, an ETS family transcription factor and key nuclear effector of the RTK signaling pathway

Cited by 20 publications

References 102 publications

Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process

Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process

Paths and Pathways that Generate Cell-Type Heterogeneity and Developmental Progression in Hematopoiesis

Receptor Tyrosine Kinases in Development: Insights from Drosophila

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