Lesson of the week: Interpretation of rubella serology in pregnancy---pitfalls and problems

Best, Jennifer M.; O’Shea, Siobhan; Tipples, Graham; Davies, Nicholas; Al-Khusaiby, Saleh M.; Krause, Amanda; Hesketh, Louise; Jin, Li; Enders, Gisela

doi:10.1136/bmj.325.7356.147

Cited by 81 publications

(45 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A study on rubella serology had been done by Jennifer M Best, Siobhan O'Shea, Graham Tipples, et al Showing false positive rubella IgM results due to cross-reaction with parvovirus infection B19 [7]. We conclude rubella false positive result can occur that require special attention, particularly in pregnancy to avoid termination of healthy one.…”

Section: Discussionmentioning

confidence: 99%

“…Other tests in limited use to detect rubellaspecific IgM include hemagglutination inhibition and immunofluorescent antibody assay [4,5]. "False positive rubella IgM test results have been reported in those with other viral infections as acute Epstein-Barr virus, infectious mononucleosis, cytomegalovirus infection, parvovirus B19 infection and the presence of rheumatoid factor'' [6,7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

False Positive Rubella Test In Pregnancy

Al-Amer¹

2017

J Women's Health Care

View full text Add to dashboard Cite

A 28 years female pregnant; 15 week +3 days gestational age, G0, P1, A0; presented for routine antenatal care visit in primary health care center. She had done the investigation in privet hospital showing rubella +ve IgM, CMV +ve IgG. Taking full history, the patient had simple flu like illness several weeks ago with no treatment is needed, no recent contact with a case of rubella nor recent vaccination. By examination, the patient is clinically normal. Ultrasound is done, no congenital anomaly, no signs of fetal infection, normal fetal wellbeing. I advise the patient to repeat the TORCH panel in 2 weeks later. The result by now; is showing rubella -ve IgM, CMV +ve IgG. Rubella false positive result can occur that require special attention to avoid termination of healthy pregnancy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

False Positive Rubella Test In Pregnancy

Al-Amer¹

2017

J Women's Health Care

View full text Add to dashboard Cite

show abstract

“…Bazı yazarlar tarafından döküntü veya döküntülü hastalığı olan kişilerle temas öyküsü olmadıkça, değerlendirmede yapılacak hatalardan dolayı, rubella taraması yapılmaması gerektiği öne sürülmüştür. 15 Gebe 22,23 Yapılan çalışmalarda IgM pozitifliği %0-3.2 arasında değişiklik göstermiştir. 4,6,12,13,18 Bizim ça-lışmamızda ise hem gebelerde hem de gebe olmayan doğurganlık çağındaki kadınlarda rubella IgG seropozitifliği %94.1 oranı ile ülkemiz verileri ile benzerdir.…”

unclassified

Investigation of Rubella antibodies among women of childbearing age in Sanliurfa province: An evaluation of three years period

Çiçek¹

2012

Dicle Med J

View full text Add to dashboard Cite

Objectives: The aim of this study was to investigate seroprevalance of antibodies against rubella virus that may lead to congenital infection among women of fertility age as a risk group that referred to Şanlıurfa Women's Health and Maternity Hospital in the period between 01. 01.2007 and 31.12.2009. Materials and methods:The results of 13.037 of rubella IgM and 12.134 of rubella IgG antibodies that investigated in the sera samples, by using the methods chemiluminescense immunoassay and electrochemiluminescence, obtained from the women in fertility age that referred to the hospital with various complaints. Results were evaluated by classifying in five age subgroups in terms of negative, border line and positive according to the manufacturer's description.Results: Totally, 13.037 women in the age range of 15-49 years (average age 28.35±7.48 years) were evaluated. Negative, positive and border line ratios of the rubella IgM antibodies were found as 98.1%, 1.7% and 0.2%, respectively. Rubella IgG seropositivity was 94.1% whereas its seronegativity rate was 5.9%. The highest rate of rubella IgG seropositivity was found in the 35-44 years age group. In comparison to the other age groups, this would be considered significantly high except up to 49 age group (p<0.001). Rubella IgG seropositivity was found only in 221 patients (1.7%). The highest seropositivity rate was found in the age range of 35-44, and this high rate was statically significant except that in the age group of 25-34 (p<0.001). Conclusion:Because of the rubella seropositivity was high in this province, it might be concluded that scanning of the antibodies to rubella in women living in Şanlıurfa was unnecessary. However, considering of the problems due to the infection during pregnancy, antibodies to rubella should be scanned in women that not vaccinated in the childhood age or previously not have the disease, and they should be offered the vaccination. Gereç ve yöntem:Çeşitli şikayetlerle hastaneye baş-vuran doğurganlık çağındaki kadınlardan alınan serum örneklerinde kemilüminesans immüno assay ve ElectroChemi Luminescence yöntemi ile çalışılan toplam 13.037 rubella IgM ve 12134 rubella IgG antikor sonuçları retrospektif olarak incelenmiştir. Sonuçlar üretici firmanın öne-rileri doğrultusunda negatif, sınır değer ve pozitif olarak sınıflandırılmış ve beş farklı yaş grubuna ayrılarak değer-lendirilmiştir. Bulgular:Çalışmaya dahil edilen yaşları 15-49 arasında değişen13.037 kadının yaş ortalaması 28.35±7.48 idi. Ça-lışılan serum örneklerinde, rubella Ig M antikorlarının negatiflik, pozitiflik, ve sınır değer oranları sırasıyla %98.1, %1.7 ve %0.2 olarak saptanmıştır. Rubella IgG pozitifliği %94.1 iken; negatiflik oranı %5.9 bulunmuştur. Rubella IgG pozitiflik oranı en yüksek 35-44 yaş grubunda bulundu ve diğer yaş grupları ile karşılaştırıldığında 49 yaş üstü grup dışında anlamlı şekilde yüksek olduğu saptandı (p<0.001). Rubella IgM pozitifliği ise sadece 221 hastada bulundu (%1.7), pozitiflik oranı en çok yine 35-44 yaş aralığında görülmüş ve ...

show abstract

“…Acute rubella infection during the first trimester of pregnancy carries a high risk of congenital rubella syndrome in the newborn. The interpretation of a positive rubella virus IgM result during pregnancy, where the implications of a false-positive or misinterpreted result are significant, is therefore particularly important (2). In addition to seeking patient history pertaining to vaccination status, recent exposure, and signs or symptoms of clinical infection, additional laboratory testing provides clinicians and patients with more information upon which to base clinical-management decisions.…”

mentioning

confidence: 99%

“…Consideration must be given, however, to the possibility of false-positive rubella virus IgM results where the prevalence of rubella is low (2,15). Also, IgM may persist beyond the expected period of 4 weeks (14).…”

mentioning

confidence: 99%

Evaluation of Commercial Rubella Immunoglobulin G Avidity Assays

2007

View full text Add to dashboard Cite

We compared the performances of five commercial rubella virus immunoglobulin G (IgG) avidity assays. The Adaltis (kappa ‫؍‬ 0.28) and Diesse (kappa ‫؍‬ 0.33) assays showed poor correlation, the Behring assay (kappa ‫؍‬ 0.68) showed good correlation, and the Euroimmun (kappa ‫؍‬ 0.95) and Radim (kappa ‫؍‬ 0.94) assays showed excellent correlation with a well-established in-house rubella virus IgG avidity assay. The Euroimmun and Radim assays were statistically significantly better than the other commercial assays (P < 0.01).

show abstract

Lesson of the week: Interpretation of rubella serology in pregnancy---pitfalls and problems

Abstract: Clinical and laboratory expertise is essenrial for evaluating rubella specific IgM test results in pregnancy

Cited by 81 publications

References 12 publications

False Positive Rubella Test In Pregnancy

False Positive Rubella Test In Pregnancy

Investigation of Rubella antibodies among women of childbearing age in Sanliurfa province: An evaluation of three years period

Evaluation of Commercial Rubella Immunoglobulin G Avidity Assays

Contact Info

Product

Resources

About