Lesiones macro y microscópicas de intestino delgado de cerdos neonatos sin calostrar inoculados experimentalmente con cepas de E. coli fimbriadas

Effect of Agroindustrial Waste Substrate Fermented with Lactic Acid Bacteria and Yeast on Changes in the Gut Microbiota of Guinea Pigs

“…In the present study, guinea pigs consuming microbial bioactive compounds containing lactic acid bacteria and yeasts improved macroscopically, which at the same time reducing the macroscopic lesions in the gastrointestinal tract wall of the stomach, small intestine, colon and cecum relative to animals in the control group ( Table 5 ). These results are consistent with the findings of Canal [ 30 ] describing macroscopic changes in the intestinal wall (mainly jejunum and ileum) in 1–7-day-old piglets infected with the E. coli strain, while others report to have observed a close relationship between pathogenic bacterial serogroups and hemolysin production.…”

“…According to other reports, the use of mixed cultures of lactic acid bacteria and yeasts, usually Lactobacillus alone or in combination with Bifidobacteria , Enterococci or Saccharomyces included in animal diets, reduces the risk of diarrhea associated with E. coli , Salmonella spp., among others [ 15 , 30 ]. While it is true that there is heterogeneity of results in terms of reducing diarrheal disorders in animals, there is insufficient evidence to say whether the effect varies systematically between the populations of microorganisms introduced in the probiotics used [ 8 , 16 , 30 ].…”

“…The pH values in the present study were lower in animals treated with microbial bioactive compounds, which may have had a positive effect on the digestive process and nutrient absorption in the gastrointestinal tract [ 39 ]. According to the literature, the maintenance of stable pH values is known to positively influence cellular homeostasis as well as nutrient absorption [ 5 , 11 , 30 ].…”

“…Conversely, alterations in luminal pH in the intestinal tract favor the development of pathogenic diseases [ 35 ]; thus, an elevated pH in the stomach and in the pyloric region of the duodenum favors the development of infections [ 38 ]. It has been shown that the pH of the small intestinal surface microclimate is the result of a dynamic balance between H + secretion and absorption across the luminal membrane (under normal conditions, H + secretion is considered to predominate over absorption) and the diffusion of mucosal fluids into the intestinal lumen [ 30 , 41 ].…”

“…The evaluation of macroscopic lesions in guinea pigs was performed by measuring the following parameters: degree of thickness of the intestinal wall, presence of circulatory disorders in the mucosa (edema, congestion, hemorrhages) and consistency of the intestinal contents (watery, mucous, foamy), according to the methodology described by Canal [ 30 ]. The pH values of the intestinal contents were measured using a digital pH meter (Hanna ® , Pandova, Italy).…”