Les probiotiques

Smoragiewicz, Wanda; Bielecka, M.; Babuchowski, A.; Boutard, Armel; Dubeau, Hélène

doi:10.1139/m93-165

Cited by 34 publications

(26 citation statements)

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“…Undesirable reactions were not produced in suckling or in adult mice during this experiment, neither in 248 piglets in the suckling phase and 199 in the nursery phase used in field tests (21). Even considering that many strains of different species of Bacillus produce toxins harmful to man and animals, some are innocuous (5) or even beneficial (18), as was demonstrated for B. cereus CenBiot in the experiments here reported.…”

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confidence: 46%

Properties of the Bacillus Cereus strain used in probiotic CenBiot

et al. 1999

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Bacillus cereus CenBiot fulfilled the requirements to be used as probiotic. The spores showed D 80 of 14 hs, inhibited Escherichia coli and Yersinia pseudotuberculosis after 24 hs in associative culture, were innocuous for suckling and adult mice and were not inhibited by antibiotics at low concentrations. Revista de Microbiologia (1999) Probiotics are "viable mono or mixed cultures of microorganisms which, applied to animal or man, beneficially affect the host by improving the properties of the indigenous flora" (10). They must promote growth, improve feed conversion and inhibit enteropathogens, without causing any undesirable effect. In addition, they must survive the stress produced during manufacturing, storage and administration at farm conditions. Key words: probiotic, Bacillus cereusThe search for new probiotic strains has increased in recent years due to the necessity to find economic and effective substitutes for antibiotics used as feed additives. Several strains of Lactobacillus, Pediococcus, Bacteroides, Bifidobacterium, Bacillus, Streptococcus and Escherichia coli, alone or consortiated, have been used as probiotics (8), giving controversial results. Among the various species of probiotics, those belonging to the genus Bacillus have the advantage that, due to their capacity to sporulate, they survive at ambient temperatures as well as during desiccation by methods that involve moderate heating, such as spray dryers, avoiding the use of lyophilization or other expensive technologies (10). This property also makes possible the administration of spores mixed with powdered vehicles instead of gels or liquids used with non sporulated bacteria. Although several strains of different species of Bacillus are being used for this purpose, information concerning their properties was seldom reported.Probiotic CenBiot, prepared with a strain of Bacillus cereus at Centro de Biotecnologia of Universidade Federal de Pelotas, Rio Grande do Sul, Brazil, was tested in commercial farms showing beneficial effects in the control of diarrhoea and feed conversion in pigs (21). The effects of some factors that could affect the survival of Bacillus cereus in the intestinal tract and during manufacturing, such as interaction with enteropathogens, resistance to heat and to variation of pH, are reported in this work.The strain was classified as Bacillus cereus due to its morphology, Gram staining, capacity to form 12 C. Gil-Turnes et al. thin wall spores in aerobic conditions, growth in glucose broth under anaerobic conditions, production of lecithinase and acetyl-methyl-carbinol and absence of urease (20). To obtain spore suspensions, cultures were heated at 80 o C for 15 minutes, centrifuged, and after three successive washings with ultrapure water, resuspended in ultrapure sterile water pH 7.2.To test the effect of heat, aliquots of 300µl of spore suspensions were heated in a water bath at 50 o C for 4 days, 80 o C for 60 min, 85 o C for 60 min and 90 o C for 40 min. Samples were collected every 24 hours, 10 min,...

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confidence: 46%

Properties of the Bacillus Cereus strain used in probiotic CenBiot

et al. 1999

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“…FAO and WHO defined probiotics as live micro-organisms that when administrated in adequate amount confer a health benefit on the host (FAO/WHO, 2001). Probiotic operation has been well defined (Fuller, 1978(Fuller, , 1992Fuller and Turvey, 1971;Parker, 1974;Roach and Tannock, 1980;Smoragiewicz et al, 1993). The aim of this study was to investigate the lengthweight relationships and growth characteristics of three-spot Gourami fish in an in vitro study under different concentrations of Bacillus circulance and Bacillus subtilis.…”

Section: Introductionmentioning

confidence: 99%

GROWTH AND LENGTH-WEIGHT RELATIONSHIP OF Trichopodus trichopterus (Pallas, 1770) FED A SUPPLEMENTED DIET WITH DIFFERENT CONCENTRATIONS OF PROBIOTIC

Jafaryan

Sahandi

Dorbadam

2014

CJF

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The aim of the present study was to evaluate the effects of diet with different microbial concentrations on the growth and length-weight relationship of Trichopodus trichopterus. For this aim a study was designed with four experimental treatments and the control to evaluate different microbial concentrations on growth and length-weight relationship. The counts of bacteria that were used for dietary supplementation were 1×10 4 , 2×10 4 , 3×10 4 and 4×10 4 CFU g -1 in T1, T2, T3 and T4 treatments, respectively. The results indicated that the use of 3×10 4 CFU g -1 concentration increased the length, and a positive allometric growth was observed. The higher length frequency was estimated in T3. The results indicated that the use of microbial additives with proper concentration causes different allometric growth.

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“…However antibiotics not only contribute to the destruction of beneficial gastrointestinal microflora but also produce residual effects in tissues and products of animal origin such as meat, eggs and milk. (Smoragiewicz et al, 1993). Probiotic has been defined as a live or dead organism from Lactobacillus, Streptococcus, Enterococcus, Bacillus and Saccharomyces genera as well as microbial fermentation products, nucleotides and its metabolizable products, metabolites and organic acids (Ouwehand et al 1999).…”

Section: Introductionmentioning

confidence: 99%