Les pièges de l’intimité

Moisset, Pierre

doi:10.1016/j.melaen.2014.05.008

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2019

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“…In the past, rheological characterization of airway mucus has been restricted to sputum or mucus from dead mammals because rheology methods require large volumes of sample (mL) and because of the difficulty of collecting human bronchial mucus. Models of human bronchial epithelium (HBE) culture have been developed these last decades [2][3][4][5]. While these models have been validated by biological factors demonstrating, for various pathologies, that the phenotype is conserved, a few studies [6][7][8] describe the physical characteristics of these models and in particular the mucus flowing properties.…”

Section: Introductionmentioning

confidence: 99%

Mucus Microrheology Measured on Human Bronchial Epithelium Culture

et al. 2019

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We describe an original method to measure mucus microrheology on human bronchial epithelium culture using optical tweezers. We probed rheology on the whole thickness of mucus above the epithelium and showed that mucus gradually varies in rheological response, from an elastic behavior close to the epithelium to a viscous one far away. Microrheology was also performed on mucus collected on the culture, on ex vivo mucus collected by bronchoscopy, and on another epithelium model. Differences are discussed and are related to mucus heterogeneity, adhesiveness, and collection method.

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