Les domaines à doigts de zinc

Dion, Sarah; Demattei, Marie-Véronique; Renault, Sylvie

doi:10.1051/medsci/20072310834

Cited by 9 publications

(2 citation statements)

References 36 publications

(41 reference statements)

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“…More recently, it has been shown that zinc finger proteins, and especially their LIM domain [26], could be involved in a mechano-sensing response [27] and could induce genetranscription regulation [28]. They would therefore be reasonable candidates to participate in a mechano-transductive process.…”

Section: Introductionmentioning

confidence: 99%

Impact of Treadmill Interval Running on the Appearance of Zinc Finger Protein FHL2 in Bone Marrow Cells in a Rat Model: A Pilot Study

Germain

Bourzac

Pichon

et al. 2022

Life

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Although the benefits of physical exercise to preserve bone quality are now widely recognized, the intimate mechanisms leading to the underlying cell responses still require further investigations. Interval training running, for instance, appears as a generator of impacts on the skeleton, and particularly on the progenitor cells located in the bone marrow. Therefore, if this kind of stimulus initiates bone cell proliferation and differentiation, the activation of a devoted signaling pathway by mechano-transduction seems likely. This study aimed at investigating the effects of an interval running program on the appearance of the zinc finger protein FHL2 in bone cells and their anatomical location. Twelve 5-week-old male Wistar rats were randomly allocated to one of the following groups (n = 6 per group): sedentary control (SED) or high-intensity interval running (EX, 8 consecutive weeks). FHL2 identification in bone cells was performed by immuno-histochemistry on serial sections of radii. We hypothesized that impacts generated by running could activate, in vivo, a specific signaling pathway, through an integrin-mediated mechano-transductive process, leading to the synthesis of FHL2 in bone marrow cells. Our data demonstrated the systematic appearance of FHL2 (% labeled cells: 7.5%, p < 0.001) in bone marrow obtained from EX rats, whereas no FHL2 was revealed in SED rats. These results suggest that the mechanical impacts generated during high-intensity interval running activate a signaling pathway involving nuclear FHL2, such as that also observed with dexamethasone administration. Consequently, interval running could be proposed as a non-pharmacological strategy to contribute to bone marrow cell osteogenic differentiation.

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Section: Introductionmentioning

confidence: 99%

Impact of Treadmill Interval Running on the Appearance of Zinc Finger Protein FHL2 in Bone Marrow Cells in a Rat Model: A Pilot Study

Germain

Bourzac

Pichon

et al. 2022

Life

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“…Il y a quelques années encore, les nucléases à doigts de zinc (en anglais zinc finger nucleases, ZFN) étaient la seule option à la disposition des chercheurs voulant modifier un génome. À la fin de 2011, une nouvelle méthode de modification des gènes, celle des TAL effector nucleases (TALEN) [45], a été découverte. Elle a été sélectionnée par la revue Nature Methods comme la « Méthode de l'année ».…”

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CRISPR, un système qui permet de corriger ou de modifier l’expression de gènes responsables de maladies héréditaires

Tremblay

2015

Med Sci (Paris)

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Les trois méthodes offrent la capacité aux chercheurs de modifier le génome des plantes, des animaux et même celui des cellules humaines [1]. La technologie CRISPR peut être utilisée en biotechnologie, pour la recherche biomédicale et pour des applications thé-rapeutiques. Elle permet de modifier de façon spécifique la séquence des nucléotides d'un gène, ou d'un élément non codant (comme un microARN), pour les rendre non fonctionnel. Elle permet également d'éliminer ou d'introduire de un à plusieurs milliers de nucléotides afin de corriger une mutation responsable de maladie héréditaire. Les modifications apportées peuvent également conduire à identifier la fonction d'un gène qui est alors rendu non fonctionnel, et à évaluer les conséquences phénotypiques de mutations induites dans des cellules ou des organismes entiers [48].

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