Les contrôles microbiologiques de l'environnement hospitalier

Microbiological Control in Hospital Environment: Calibrating Enumeration of Bacteria on Flat Surfaces with Wet Swab

“…During sampling, microbial populations encompassing viable bacteria and bacteria stressed by the environment or damaged by the mechanical action of handling are extracted together. The stressed or damaged organisms are thought to be amongst factors that reduce the growth yields, expressed as low count in colony forming units (Hartemann et al, 1997;Moor andGriffith, 2002a,b, 2007). In some cases, however, this factor would have no impact.…”

“…This finding primarily suggests that viability would not have a significant role detection rates. This growth capacity may also be impacted by the type of bacterial growth medium used which may be poor or rich in nutrients (Hartemann et al, 1997;Moor andGriffith, 2002a,b, 2007). Some authors believe that the dryness of a surface is a factor that increases bacterial adhesion and would explain the difficulty in extraction during sampling.…”

“…Their presence increases the likelihood of healthcare-associated infections and/or the risk of spreading hospital germs into communities (Bertrou et al, 2000;Cavallo et al, 2002;Dancer, 2014;Tchoukoua et al, 2018). For the microbial reservoir character of healthcare institution environment (Hartemann et al, 1997;Meunier et al, 2005;Dancer, 2011;Doll et al, 2018), environmental microbiology monitoring is necessary and stands to be one of the measurement tools to assess the microbiological status, the efficiency of hygiene methods useful in designing and implementing corrective measures. These controls may be carried out as part of a facility qualification procedure or may be used as investigation for educational purposes (Cavallo et al, 2002).…”

“…For microbiological monitoring of surfaces, one of the analytical methods used and/or suggested by some authors is based on swabbing (Bertrou et al, 2000;Boulestreau et al, 2016;Tchoukoua et al, 2018;Fotsing Kwetché et al, 2020). However, some authors report a number of factors influencing the reliability related making it difficult to assess the real surface colonization (Hartemann et al, 1997;Moor andGriffith 2002 a, b, 2007;Keeratipibul et al, 2017). Thus, for better monitoring using this method on the basis of data provided by previous authors (Hartemann et al, 1997;Moor andGriffith 2002a,b, 2007;Keeratipibul et al, 2017), a benchmark to help predicting accurately the bacterial loads based on the enumeration result subsequent to microbial culture is necessary and should be developed.…”

“…However, some authors report a number of factors influencing the reliability related making it difficult to assess the real surface colonization (Hartemann et al, 1997;Moor andGriffith 2002 a, b, 2007;Keeratipibul et al, 2017). Thus, for better monitoring using this method on the basis of data provided by previous authors (Hartemann et al, 1997;Moor andGriffith 2002a,b, 2007;Keeratipibul et al, 2017), a benchmark to help predicting accurately the bacterial loads based on the enumeration result subsequent to microbial culture is necessary and should be developed. The purpose of the present investigation was primarily to determine the relationship between these two parameters for a wet swabbased analysis method in order to improve the understanding of colonization of surfaces in a variety of environments, particularly in healthcare facilities.…”