Les cellules souches de la pulpe dentaire : caractéristiques,
          cryopréservation et potentialités thérapeutiques

Collart-Dutilleul, Pierre-Yves; Thonat, Catherine; P, Jacquemart; Cuisinier, Frédéric; Levallois, Bernard; Chaubron, Franck

doi:10.1051/orthodfr/2012020

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…Dental pulp is an adequate source of mesenchymal stem cells that have recently been the focus of attention because of the abundance of cells in each tooth and convenient isolation methods from other tissue sources of stem cells (Collart-Dutilleul et al, 2015). Pulp tissue of human third molar teeth is an available source of MSCs.…”

Section: Discussionmentioning

confidence: 99%

Neural Differentiation of Wisdom Tooth Follicle Stem Cells on a Nano-Hydrogel Scaffold Containing Salvia Chloroleucat to Treat Nerve injury in the Cancer of Nervous System

Bahrami

Manafi

Mohammadi

et al. 2023

Asian Pac J Cancer Prev

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Section: Discussionmentioning

confidence: 99%

Neural Differentiation of Wisdom Tooth Follicle Stem Cells on a Nano-Hydrogel Scaffold Containing Salvia Chloroleucat to Treat Nerve injury in the Cancer of Nervous System

Bahrami

Manafi

Mohammadi

et al. 2023

Asian Pac J Cancer Prev

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“…Biobanks come in various types (a) to cater to public needs (Gottweis and Lauss, 2011;Hauskeller, 2016, 2018), (b) for private or family use (Santoro, 2011), or (c) for community use. Tissue samples with respect to stem cells include blood (Starr, 2002), cord blood (Wagner and Gluckman, 2010), menstrual blood (Fannin, 2013), peripheral blood, and dental pulp (Collart-Dutilleul et al, 2015). Cord blood stored in biobanks is a potential source for hematopoietic (blood-producing) stem cells.…”

Section: Introductionmentioning

confidence: 99%

Thalassemia, biobanking infrastructures, and personalized stem cell therapies in Chennai

Panwar¹

2023

Front. Sociol.

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Thalassemia and leukemia and related blood disorders are approved for blood stem cell transplants in India, for a stem cell transplant to be successful, the human leukocyte antigen (HLA) complex located on the arm of chromosome six must be a match between the cord blood donor and the recipient. In the quest to find an exact blood stem cell match for an individual, the HLA becomes the node at the center of community genetics where the HLA match is sought (not necessarily successful) in the extended family, the same caste, language, and ethnic (both national and the diaspora) groups. By considering thalassemia as a case study, how do we understand personalized stem cell therapies within biobanking infrastructures in Chennai? How do social categories get entwined with biological materials like cord blood?

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Serum-Containing Medium Effect on Isolation Rate of Dental Pulp Cells from Cryopreserved Intact Deciduous Teeth

Werle

Lindemann

Machado

et al. 2014

Journal of Clinical Pediatric Dentistry

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Objectives: To isolate cells from pulp of intact cryopreserved deciduous teeth. The null hypothesis raised here is to find no difference in the establishment of cell culture after cryopreservation (1) using culture medium supplemented with different concentrations of fetal bovine serum (FBS); and (2) between teeth with different stages of physiological root resorption. Study design: Intact deciduous teeth with different root resorption stages were cryopreserved using FBS and Dimethyl Sulfoxide (DMSO) medium (9:1) in a progressive freezing process, by placing the samples in the refrigerator (4°C/60 min) and subsequently transferring them to a -80°C freezer (controlled device -1°C/min/24 hours), and finally into liquid nitrogen (-196°C/30 days). After the thawing process, the cell isolation was performed by enzymatic digestion (type I collagenase). The cells were re-suspended into the culture medium with 10% (G1) or 20% (G2) of FBS. Microscopic analysis was performed after 30 days to visualize the cell attachment. Results: The culture establishment rate was higher in G2 (75%) than G1 (12.5%) (p=0.041). There was no difference between the different stages of root resorption. Conclusions: It was possible to establish cell cultures from the pulp of intact cryopreserved deciduous teeth. The use of 20% FBS after thawing improved the culture rate.

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Les cellules souches de la pulpe dentaire : caractéristiques, cryopréservation et potentialités thérapeutiques

Cited by 5 publications

References 30 publications

Neural Differentiation of Wisdom Tooth Follicle Stem Cells on a Nano-Hydrogel Scaffold Containing Salvia Chloroleucat to Treat Nerve injury in the Cancer of Nervous System

Neural Differentiation of Wisdom Tooth Follicle Stem Cells on a Nano-Hydrogel Scaffold Containing Salvia Chloroleucat to Treat Nerve injury in the Cancer of Nervous System

Thalassemia, biobanking infrastructures, and personalized stem cell therapies in Chennai

Serum-Containing Medium Effect on Isolation Rate of Dental Pulp Cells from Cryopreserved Intact Deciduous Teeth

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