Les agents de régénération (ou RGTAs) : une nouvelle approche thérapeutique

Barritault, Denis; Caruelle, Jean‐Pierre

doi:10.1016/s0003-4509(06)75306-8

Cited by 37 publications

(6 citation statements)

References 30 publications

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“…Affinity procedures have been developed to isolate HS subfractions for specific therapeutic applications in bone and vascular repairs and in the expansion of progenitor MSCs for regenerative procedures. These include BMP-2 interactive HS, 217,218 RGTA biomimetic HS, 219 affinity-purified HS, 220 and engineered HS samples 221 for enhanced bone repair. HA-GDF-5 hydrogels have also been developed for bone repair.…”

Section: Introductionmentioning

confidence: 99%

Glycosaminoglycans in Wound Healing

Melrose

2016

Bone�Tissue�Regen�Insights

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ABSTR ACT: Glycosaminoglycans (GAGs) are ancient molecules that have persisted throughout vertebrate and invertebrate evolution for many hundreds of millions of years. They have essential roles in the regulation of many fundamental physiological processes, which control cellular proliferation, differentiation, matrix assembly, homeostasis, and cellular behavior, which has ensured the survival of vertebrates and invertebrates to the present day. In this study, we review the roles of GAGs in wound repair in a number of tissues. The healing of some wounds such as diabetic ulcers, cartilaginous defects, and damaged neural tissue can be a particularly demanding clinical problem. However, with the directive roles that GAGs have established through evolution, the development of key matrix and cellular molecules to control cellular behavior has resulted in the development of sophisticated control systems to regulate key physiological life-preserving processes. The control of wound repair is but one example of these. With a greater understanding of how GAGs actually do this, inroads are being made in the application of this information in the development of therapeutic procedures to improve repair of these problematic tissues. The application of engineered heparin sulfate (HS), human natural killer-1 (HNK-1), and HS biomimetics in neuromuscular repair biology, activated protein C in the treatment of diabetic ulcers, and affinity-isolated HS subfractions for specific applications in bone and vascular repair and in the improved expansion of mesenchymal stem cells for therapeutic applications represent significant advances in wound repair biology and emphasize the importance of GAGs in these processes. Armed with a greater understanding of the glycocode and the roles of GAGs in such repair processes, further improvements in therapeutic interventions are likely in the area of repair biology.

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Section: Introductionmentioning

confidence: 99%

Glycosaminoglycans in Wound Healing

Melrose

2016

Bone�Tissue�Regen�Insights

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“…In this way, OTR4120 offers a matrix therapy that restores the natural cellular microenvironment and the endogenous signaling of cell communications needed for tissue regeneration (8–10). This facilitates the quality of healing by potentiating the intrinsic tissue regeneration.…”

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confidence: 99%

Diabetes-Impaired Wound Healing Is Improved by Matrix Therapy With Heparan Sulfate Glycosaminoglycan Mimetic OTR4120 in Rats

et al. 2012

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Wound healing in diabetes is frequently impaired, and its treatment remains a challenge. We tested a therapeutic strategy of potentiating intrinsic tissue regeneration by restoring the wound cellular environment using a heparan sulfate glycosaminoglycan mimetic, OTR4120. The effect of OTR4120 on healing of diabetic ulcers was investigated. Experimental diabetes was induced by intraperitoneal injection of streptozotocin. Seven weeks after induction of diabetes, rats were ulcerated by clamping a pair of magnet disks on the dorsal skin for 16 h. After magnet removal, OTR4120 was administered via an intramuscular injection weekly for up to 4 weeks. To examine the effect of OTR4120 treatment on wound heal-ing, the degree of ulceration, inflammation, angiogenesis, and collagen synthesis were evaluated. We found that OTR4120 treatment significantly reduced the degree of ulceration and the time of healing. These effects were associated with reduced neutrophil infiltration and macrophage accumulation and enhanced angiogenesis. OTR4120 treatment also increased the collagen content with an increase of collagen type I biosynthesis and reduction of collagen type III biosynthesis. Moreover, restoration of the ulcer biomechanical strength was significantly enhanced after OTR4120 treatment. This study shows that matrix therapy with OTR4120 improves diabetes-impaired wound healing.

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“…In this sense, HSmP has been previously reported to have an antifibrotic effect by decreasing collagen III synthesis and improving collagen reorganization. HSmP also inhibits proteolytic enzymes in vitro [60]. In addition, the relatively reduced number of proliferative cells found in mice treated with HSmP may contribute to reducing the amount of myofibroblasts in a similar way to that using mitomycin C, which successfully reduces scarring after PRK due to its antineoplastic properties [57].…”

Section: Discussionmentioning

confidence: 99%

Synthetic Heparan Sulfate Mimetic Polymer Enhances Corneal Nerve Regeneration and Wound Healing after Experimental Laser Ablation Injury in Mice

et al. 2022

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(1) Background: Abnormal corneal wound healing compromises visual acuity and can lead to neuropathic pain. Conventional treatments usually fail to restore the injured corneal tissue. In this study, we evaluated the effectiveness of a synthetic heparan sulfate mimetic polymer (HSmP) in a mouse model of corneal wound healing. (2) Methods: A surgical laser ablation affecting the central cornea and subbasal nerve plexus of mice was used as a model of the wound-healing assay. Topical treatment with HSmP was contrasted to its vehicle and a negative control (BSS). Corneal repair was studied using immunofluorescence to cell proliferation (Ki67), apoptosis (TUNEL assay), myofibroblast transformation (αSMA), assembly of epithelial cells (E-cadherin) and nerve regeneration (β-tubulin III). (3) Results: At the end of the treatment, normal epithelial cytoarchitecture and corneal thickness were achieved in HSmP-treated animals. HSmP treatment reduced myofibroblast occurrence compared to eyes irrigated with vehicle (p < 0.01) or BSS (p < 0.001). The HSmP group showed 50% more intraepithelial nerves than the BSS or vehicle groups. Only HSmP-treated corneas improved the visual quality to near transparent. (4) Conclusions: These results suggest that HSmP facilitates the regeneration of the corneal epithelium and innervation, as well as restoring transparency and reducing myofibroblast scarring after laser experimental injury.

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Les agents de régénération (ou RGTAs) : une nouvelle approche thérapeutique

Cited by 37 publications

References 30 publications

Glycosaminoglycans in Wound Healing

Glycosaminoglycans in Wound Healing

Diabetes-Impaired Wound Healing Is Improved by Matrix Therapy With Heparan Sulfate Glycosaminoglycan Mimetic OTR4120 in Rats

Synthetic Heparan Sulfate Mimetic Polymer Enhances Corneal Nerve Regeneration and Wound Healing after Experimental Laser Ablation Injury in Mice

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