“…Similarly, Koetsveld and co-workers used the DFM to count motile spirochetes to help accomplish the antimicrobial susceptibility of clinical isolates of Borrelia miyamotoi . In addition, the motility count by DFM was also used for the evaluation of syphilis vaccine candidates. , Furthermore, DFM has also been used in the diagnosis of Leptospirosis, which appeared in a case report of Leptospira infections . Interestingly, DFM was also used in the preferences for blood groups of ticks, and positive results were obtained…”