Leptospira DNA detection for the diagnosis of human leptospirosis

A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine

“…Moreover, the Leptospira type (species and/or serovar) and infection dose influence the clinical outcome, which may result in excretion of varied numbers of leptospires in urine. However, leptospiral DNA has been detected by PCR in urine samples collected from patients in the acute phase with a higher sensitivity than that in blood samples (1,3,6). Although further clinical validation is required, we also detected leptospiral rrs in urine from humans and dogs with acute leptospirosis (data not shown), indicating the applicability of Lepto-rrs LAMP for early diagnosis of leptospirosis.…”

“…Leptospiral DNA has been detected by PCR during the chronic phase in urine of carrier animals and during the early phase in patients with leptospirosis (1,3,6,13). Because urine collection is easy and less invasive than blood collection, we attempted to establish a new LAMP method, Lepto-rrs LAMP, which uses simplified sample processing to detect leptospiral DNA in urine.…”

Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia

“…With its specificity and rapidity, PCR has become a promising tool for the early detection of Leptospira in various specimens. 12,13,33 However, MAT was able to identify successfully 20 positive Leptospira isolates at the serogroup level using 25 different sera. Two serogroups were identified; serogroup Javanica was found to be the predominant serogroup infecting 17 (85%) positive rats, whereas serogroup Bataviae was found only in 3 rats.…”

“…PCR has been successfully applied as a rapid, sensitive, and specific tool for the detection of several microorganisms, including Leptospira, 11 in a variety of specimens from different hosts. [12][13][14] The rapidity and reproducibility of pulsed-field gel electrophoresis (PFGE) makes it a very useful technique for typing Leptospira strains. 15 PFGE is able to discriminate between closely related serovars of the Leptospira spp., which may aid in the identification of the different strains at serovar level, 16 and this identification is important in understanding the epidemiology of leptospirosis in both humans and animals in any geographic region.…”

Fatal Co-Infection–Melioidosis and Leptospirosis

“…Polymerase chain reaction was carried out using G1/G2 primers, as described by Fonseca Cde and others. 10 This PCR technique only amplified the conserved sequences of L. interrogans and therefore was unable to differentiate the serovars of leptospirosis. The information on serovar is indeed helpful for epidemiological purposes but rapid diagnosis of the infection was of utmost priority in this event.…”