Leptin modulates oocyte maturation by a central and a direct pathway in zebrafish

Lakshminarasimha, Amrutha Bagivalu; Puvvada, Madhuri; Hammerschmidt, Matthias; Michel, Maximilian

doi:10.1530/joe-22-0026

Cited by 6 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A lesser studied aspect of pituitary function is that of leptin action via STAT, ELK/SRF expression targets ( Table 3 ). In fish, leptin can regulate prolactin (PRL) ( 11 ) and the gonadotropins ( 12 ). We also noted that enzymatic processing of classical hormones such as PRL can give rise to angiogenic peptides ( 13 , 14 ) that were also found in the pituitary gland.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples

Lu,

Peng,

Erandani

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

The detection and quantification of hormones are important to assess the reproductive and stress status of experimental models and for the diagnosis of diseases in human and veterinary clinics. Traditionally, steroid, peptide, and protein hormones are analyzed in individual experiments using different extraction methodologies. With the new advancement on HPLC sorbents, the simultaneous measurement of hormones from different categories becomes possible. In this study, we present a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins using high-resolution liquid chromatography tandem mass spectrometry. We demonstrate the sensitivity of our method for small tissues by acquiring data from brain, pituitary gland, and gonads of single zebrafish samples. This approach promises to shed light on the hormonal pathways and their interrelationships, providing knowledge on the integration of hormone systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples

Lu,

Peng,

Erandani

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…Higher plasma levels of leptin have been described in FL2 mice in comparison to the other lines [ 14 ]. Leptin is linked with increased fat intake, but it is also a modulator of oocyte maturation, as higher leptin levels lead to increased rates of GVBD (germinal vesicle breakdown) and decreased rates of atresia [ 42 ]. In our opinion, this could help to explain the differences between FL1 and FL2 in terms of fat content ( Figure 2 ), but also the presence of POFs [ 43 ] predominant in FL1 where the GVBD rate could be lower.…”

Section: Discussionmentioning

confidence: 99%

Lower Plasmatic Levels of Saturated Fatty Acids and a Characteristic Fatty Acid Composition in the Ovary Could Contribute to the High-Fertility Phenotype in Dummerstorf Superfertile Mice

Calanni-Pileri

Weitzel

Dannenberger

et al. 2022

IJMS

View full text Add to dashboard Cite

In recent decades, fertility traits in humans as well as in farm animals have decreased worldwide. As such, it is imperative to know more about the genetics and physiology of increased or high fertility. However, most of the current animal models with reproductive phenotypes describe lower fertility or even infertility (around 99%). The “Dummerstorf high-fertility lines” (FL1 and FL2) are two unique mouse lines selected for higher reproductive performances, more specifically for higher number of pups per litter. We recently described how those superfertile mice managed to increase their reproductive phenotype by doubling the ovulation rate and consequently the litter size compared to the unselected mice of the same founder population. FLs show an unusual estrous cycle length and atypical levels of hormones that link reproduction and metabolism, such as insulin in FL1 and leptin in FL2. Moreover, we described that their higher ovulation rate is mostly due to a higher quality of their oocytes rather than their sheer quantity, as they are characterized by a higher quantity of high-quality oocytes in antral follicles, but the quantity of follicles per ovary is not dissimilar compared to the control. In the present study, we aimed to analyze the lipid composition of the fertility lines from plasma to the gonads, as they can connect the higher reproductive performances with their metabolic atypicalities. As such, we analyzed the fat content of FLs and fatty acid composition in plasma, liver, fat, oocytes of different quality, and granulosa cells. We demonstrated that those mice show higher body weight and increased body fat content, but at the same time, they manage to decrease the lipid content in the ovarian fat compared to the abdominal fat, which could contribute to explaining their ovarian quality. In addition, we illustrate the differences in fatty acid composition in those tissues, especially a lower level of saturated fatty acids in plasma and a different lipid microenvironment of the ovary. Our ongoing and future research may be informative for farm animal biology as well as human reproductive medicine, mostly with cases that present characteristics of lower fertility that could be reversed following the way-of-managing of Dummerstorf high-fertility lines.

show abstract

Leptin signaling promotes blood vessel formation in the Xenopus tail during the embryo-larval transition

Curtis,

Reeve,

Crespi

2024

Developmental Biology

View full text Add to dashboard Cite

Leptin modulates oocyte maturation by a central and a direct pathway in zebrafish

Cited by 6 publications

References 0 publications

Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples

Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples

Lower Plasmatic Levels of Saturated Fatty Acids and a Characteristic Fatty Acid Composition in the Ovary Could Contribute to the High-Fertility Phenotype in Dummerstorf Superfertile Mice

Leptin signaling promotes blood vessel formation in the Xenopus tail during the embryo-larval transition

Contact Info

Product

Resources

About