Leptin improves in-vitro maturation of goat oocytes through MAPK and JAK2/STAT3 pathways and affects gene expression of cumulus cells

Costa, Joana Amélia de Senna; Cezar, Guilherme Arruda; Monteiro, P. L. J.; Silva, Diogo Manoel Farias; Silva, Robespierre Augusto Joaquim Araújo; Bartolomeu, Cláudio Coutinho; Filho, Antônio Santana dos Santos; Wischral, Áurea; Batista, André Mariano

doi:10.1016/j.repbio.2022.100609

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…In mammals, leptin regulates energy metabolism and food intake by activating the long-form leptin receptor and downstream signaling ( He et al, 2012 ). Studies have shown that leptin is bound to the leptin receptor, which allowed the activation of JAK2 and modulated leptin receptor signal transduction ( Luo et al, 2021 ; Costa et al, 2022 ). Leptin-induced PI3K signaling has important consequences for food intake and sympathetic nerve activity ( Garcia-Galiano et al, 2019 ).…”

Section: Leptin Receptor and Signaling Pathwaymentioning

confidence: 99%

Current knowledge of leptin in wound healing: A collaborative review

Chen

Liao²,

Zheng³

et al. 2022

Front. Pharmacol.

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Efficacious wound healing is still a major concern for global healthcare due to the unsatisfactory outcomes under the current treatments. Leptin, an adipocyte-derived hormone, mainly acts in the hypothalamus and plays crucial roles in various biological processes. Recently, an increasing number of researches have shown that leptin played an important role in the wound healing process. In this review, we presented a first attempt to capture the current knowledge on the association between leptin and wound healing. After a comprehensive review, the molecular mechanisms underlying leptin in wound healing were speculated to be correlated to the regulation of inflammation of the macrophage and lymphocytes, angiogenesis, re-epithelialization, proliferation, and differentiation of fibroblasts. The affected genes and the signal pathways were multiple. For example, leptin was reported to ameliorate wound healing by its anti-inflammatory action, which might be correlated to the activation STAT1 and STAT3 via p38 MAPK or JAK2. However, the understanding of the specific role in each process (e.g., inflammatory, proliferative, and maturation phase) of wound repair is not entirely clear, and further studies are still warranted in both macrostructural and microscale factors. Therefore, identifying and validating the biological mechanisms of leptin in wound healing is of great significance to develop potential therapeutic targets for the treatment of wound healing in clinical practice.

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Section: Leptin Receptor and Signaling Pathwaymentioning

confidence: 99%

Current knowledge of leptin in wound healing: A collaborative review

Chen

Liao²,

Zheng³

et al. 2022

Front. Pharmacol.

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Candidate genes and signature of selection associated with different biological aspects and general characteristics of goat

Saleh¹,

Rashad²,

Hassanine³

et al. 2022

Emerging Animal Species

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Identification and Functional Analysis of circRNAs during Goat Follicular Development

Liu,

Guo,

et al. 2024

IJMS

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Litter size is a crucial quantitative trait in animals, closely linked to follicular development. Circular RNA (circRNA), a type of single-stranded closed-loop endogenous RNA with stable expression, plays pivotal roles in various biological processes, yet its function in goat follicular development remains unclear. In this study, we collected large (follicle diameter > 3 mm) and small (1 mm < follicle diameter < 3 mm) follicles from black goats in the Chuanzhong region for circRNA sequencing, with the aim of elucidating the functional circRNAs that influence follicle development in goats. Differential analysis revealed that 17 circRNAs were upregulated in large follicles, and 28 circRNAs were upregulated in small follicles. Functional enrichment analysis revealed significant enrichment of pathways related to reproduction, including cellular response to follicle-stimulating hormone stimulus, the PI3K-Akt signaling pathway, the MAPK signaling pathway, and the Notch signaling pathway. Based on the ceRNA mechanism, 45 differentially expressed circRNAs were found to target and bind a total of 418 miRNAs, and an intercalation network including miR-324-3p (circRNA2497, circRNA5650), miR-202-5p (circRNA3333, circRNA5501), and miR-493-3p (circRNA4995, circRNA5508) was constructed. In addition, conservation analysis revealed that 2,239 circRNAs were conserved between goats and humans. Prediction of translation potential revealed that 154 circRNAs may potentially utilize both N6-methyladenosine (m6A) and internal ribosome entry site (IRES) translation mechanisms. Furthermore, the differential expression and circularization cleavage sites of five circRNAs were validated through RT-qPCR and DNA sequencing. Our study constructed a circRNA map in goat follicle development, offering a theoretical foundation for enhancing goat reproductive performance.

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Leptin improves in-vitro maturation of goat oocytes through MAPK and JAK2/STAT3 pathways and affects gene expression of cumulus cells

Cited by 3 publications

References 31 publications

Current knowledge of leptin in wound healing: A collaborative review

Current knowledge of leptin in wound healing: A collaborative review

Candidate genes and signature of selection associated with different biological aspects and general characteristics of goat

Identification and Functional Analysis of circRNAs during Goat Follicular Development

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