Leptin effect on RANKL and OPG expression in MC3T3‐E1 osteoblasts

Lamghari, Meriem; Tavares, Lígia; Camboa, N.; Barbosa, Mário A.

doi:10.1002/jcb.20853

Cited by 47 publications

(38 citation statements)

References 32 publications

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“…We would also like to emphasise the role of VISF and APE in the regulation of bone remodelling in these patients. In vitro investigations into the effect of LP [26,27,[31][32][33][34][35]74], ADIPO [36][37][38]74], RES [39], VISF [40] and APE [41,42] on bone tissue cells are consistent with the above hypothesis. Stepwise regression analysis carried out in our patients revealed that VISF was an independent predictor of OC.…”

Section: Prace Oryginalnesupporting

confidence: 77%

“…It has also been suggested that the latter might regulate bone remodelling both directly and indirectly, i.e. via affecting OPG and/or RANKL expression [24,25,31,38,45,56].…”

Section: Prace Oryginalnementioning

confidence: 99%

“…Several findings [4,8,56,66] indicate a relationship between LP and/or ADIPO and RES and bone status. In vitro studies [4,31,38,45] seem to suggest that the above mentioned adipokines, and especially LP and ADIPO, modify bone metabolism, most probably via their effect on RANKL and/or OPG expression.…”

Section: Prace Oryginalnementioning

confidence: 99%

“…Leptin enhances bone mineralisation [26][27][28] and inhibits osteoclast generation in cultures of murine bone marrow stromal cells [26][27][28], but does not affect mature osteoclast differentiation [26]. It also increases OPG expression in stromal cells and osteoblasts [29,30] and/or decreases the expression of RANKL [27,30,31]. Leptin acts to enhance in vitro differentiation of human bone marrow stem cells to osteoblasts, stimulates de novo collagen synthesis and bone matrix mineralisation [32][33][34] and inhibits adipogenesis [32].…”

Section: Introductionmentioning

confidence: 99%

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Metabolizm kostny, osteoprotegeryna i ligand receptora aktywatora czynnika jądrowego-kB a wybrane hormony tkanki tłuszczowej u dziewcząt z jadłowstrętem psychicznym

Ostrowska¹,

Ziora²,

Oświęcimska³

et al. 2014

Endokrynologia Polska

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Section: Prace Oryginalnesupporting

confidence: 77%

“…It has also been suggested that the latter might regulate bone remodelling both directly and indirectly, i.e. via affecting OPG and/or RANKL expression [24,25,31,38,45,56].…”

Section: Prace Oryginalnementioning

confidence: 99%

Section: Prace Oryginalnementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolizm kostny, osteoprotegeryna i ligand receptora aktywatora czynnika jądrowego-kB a wybrane hormony tkanki tłuszczowej u dziewcząt z jadłowstrętem psychicznym

Ostrowska¹,

Ziora²,

Oświęcimska³

et al. 2014

Endokrynologia Polska

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“…Western blot analysis also confirmed the expression of Ob-Ra and Ob-Rb in MMSC3 cells [30] (Figure 4B). …”

Section: Expression Of Leptin and Leptin Receptor In Mouse Bone Marromentioning

confidence: 55%

Possible Involvement of Leptin in the Elevated Osteoblastic Activity Observed in High Turnover Type Osteoporosis of Ovariectomized Mice

Tezuka¹

2014

J Autacoids

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Postmenopausal osteoporosis is a high turnover type of osteoporosis induced by estrogen deficiency following menopause. In this type of osteoporosis, the osteoblastic activity is known to be elevated even though bone resorption by osteoclasts eventually exceeds bone formation by osteoblasts, resulting in the deterioration of the bone structure. Although the mechanisms underlying the progression of bone resorption in this disease are relatively well understood, the mechanisms underlying the elevated osteoblastic activity are yet to be elucidated. The purpose of this study is to investigate the possibility that leptin, a 16 kDa circulating hormone secreted mainly by white adipose tissue, is involved in the development and/or progression of the high turnover type of osteoporosis. Immunohistochemical analysis and ELISA were used to examine the expression of leptin in bones of ovariectomized mice. To investigate the effect of leptin on proliferation and osteoblastic differentiation of bone marrow stromal cells, cell proliferation assay and real-time RT-PCR analysis were performed using a mouse bone marrow stromal cell line, MMSC3. Immunohistochemistry and ELISA revealed enhanced expression of leptin in bone marrows of ovariectomized mice. A cell proliferation assay detected no significant effect of leptin on the proliferation of MMSC3 cells. In contrast, real-time RT-PCR revealed that leptin promoted the osteoblastic differentiation of this cell line. Estrogen depletion caused by ovariectomy induces the upregulation of leptin expression in the bone marrow cavity, which leads to the elevated osteoblastic activity observed in the early phase of high turnover type osteoporosis of ovariectomized mice.

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Peripheral leptin regulates bone formation

Turner

Kalra

Wong

et al. 2012

Journal of Bone and Mineral Research

221

247

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Substantial evidence does not support the prevailing view that leptin, acting through a hypothalamic relay, decreases bone accrual by inhibiting bone formation. To clarify the mechanisms underlying regulation of bone architecture by leptin, we evaluated bone growth and turnover in wild type (WT) mice, leptin receptor-deficient db/db mice, leptin-deficient ob/ob mice and ob/ob mice treated with leptin. We also performed hypothalamic leptin gene therapy to determine the effect of elevated hypothalamic leptin levels on osteoblasts. Finally, to determine the effects of loss of peripheral leptin signaling on bone formation and energy metabolism, we used bone marrow (BM) from WT or db/db donor mice to reconstitute the hematopoietic and mesenchymal stem cell compartments in lethally irradiated WT recipient mice. Decreases in bone growth, osteoblast-lined bone perimeter and bone formation rate were observed in ob/ob mice and greatly increased in ob/ob mice following subcutaneous administration of leptin. Similarly, hypothalamic leptin gene therapy increased osteoblast-lined bone perimeter in ob/ob mice. In spite of normal osteoclast-lined bone perimeter, db/db mice exhibited a mild but generalized osteopetrotic-like (calcified cartilage encased by bone) skeletal phenotype and greatly reduced serum markers of bone turnover. Tracking studies and histology revealed quantitative replacement of BM cells following BM transplantation. WT mice engrafted with db/db BM did not differ in energy homeostasis from untreated WT mice or WT mice engrafted with WT BM. Bone formation in WT mice engrafted with WT BM did not differ from WT mice, whereas bone formation in WT mice engrafted with db/db cells did not differ from the low rates observed in untreated db/db mice. In summary, our results indicate that leptin, acting primarily through peripheral pathways, increases osteoblast number and activity.

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Leptin effect on RANKL and OPG expression in MC3T3‐E1 osteoblasts

Cited by 47 publications

References 32 publications

Metabolizm kostny, osteoprotegeryna i ligand receptora aktywatora czynnika jądrowego-kB a wybrane hormony tkanki tłuszczowej u dziewcząt z jadłowstrętem psychicznym

Metabolizm kostny, osteoprotegeryna i ligand receptora aktywatora czynnika jądrowego-kB a wybrane hormony tkanki tłuszczowej u dziewcząt z jadłowstrętem psychicznym

Possible Involvement of Leptin in the Elevated Osteoblastic Activity Observed in High Turnover Type Osteoporosis of Ovariectomized Mice

Peripheral leptin regulates bone formation

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