Leptin Decreases Angiogenic Factors in the Developing Porcine Corpus Luteum.

Katchko, Robin A.; Wiles, Jessica R.; Aguirre, Elizabeth A.; O’Gorman, Chad; Keisler, D.H.; Stanko, R. L.; Garcia, Michelle

doi:10.1093/biolreprod/81.s1.578

Cited by 4 publications

(12 citation statements)

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“…However, FGF2 appears to exhibit a greater influence on luteal angiogenesis than VEGF [1,3]. Moreover, in the porcine CL, only FGF2 gene expression exhibits significant daily changes throughout development, increasing on days 4 and 6 and decreasing on days 5 and 7 in the early stage of the luteal phase [4]. The daily expression of FGF2 in the developing CL has not been reported in other species; however, the anti-angiogenic glycoprotein thrombospondin exhibits a similar pattern of expression in granulosa cells of antral follicles [5].…”

Section: Introductionmentioning

confidence: 93%

“…All animal-management practices and experimental procedures were previously published [4,7,9] and approved by the Texas A&M University-Kingsville (TAMUK) Institutional Animal Care and Use Committee (IACUC No. 2014-07-10).…”

Section: Animalsmentioning

confidence: 99%

“…Luteal tissue was processed as previously reported [4,7]. Briefly, tissue was mechanically minced and enzymatically dissociated in digestion media (Type IA collagenase 1.27 mg/mL, 0.5% bovine serum albumin (BSA) in Dulbecco's Modified Eagle's Medium (DMEM); Sigma Aldrich) for 90 min at 37 • C with gentle agitation in a shaking water bath.…”

Section: Dispersed Corpora Lutea Cell Culturesmentioning

confidence: 99%

“…Serum and culture media were analyzed for progesterone concentration by radioimmunoassay (RIA, Coat-A-Count ® ; Diagnostics Product Corporation, Los Angeles, CA, USA) to confirm cell viability during the culture process, response to culture treatment, and the presence of a functional CL at the time of tissue harvest. Utilization of the RIA kit was previously validated for porcine serum and culture media [4,7,38,39]. Inter-and intra-assay coefficients of variation for all assays were <10%.…”

Section: Radioimmunoassaymentioning

confidence: 99%

“…Leptin is a potent adipokine with angiogenic properties [6] that regulates the expression of FGF2 and Ang1 in dispersed lutea cell cultures, specifically decreasing FGF2 in cells obtained from D7 lutea [4]. Both leptin and its receptor are expressed in the bovine, caprine, and porcine CL [4,7,8], but changes relative to the day of development have not been detected despite a reported association with luteal angiogenesis [7,9]. Interestingly, another adipokine, adiponectin, is also expressed in the porcine CL and influences luteal function [10].…”

Section: Introductionmentioning

confidence: 99%

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Adiponectin Influences FGF2 in the Developing Porcine Corpus Luteum

Flores

Ramirez

Ayala

et al. 2022

Veterinary Sciences

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Luteal angiogenesis is regulated by pro-angiogenic hormones including fibroblast growth factor 2 (FGF2) and angiopoietin 1 (Ang1), which are regulated by the adipokine leptin during development. Another adipokine, adiponectin, exhibits an inverse relationship with leptin and has been identified in the CL. Therefore, it is hypothesized that adiponectin will influence pro-angiogenic hormones in the developing porcine CL. Crossbred sows were randomly allocated to one of two days of the estrous cycle, day 5 (D5; n = 4) or day 7 (D7; n = 5) for CL collection. Tissue was processed for immunohistochemical localization of adiponectin receptor 2 (AdipoR2), gene expression of FGF2, Ang1, leptin, AdipoR2, and cell culture for adiponectin treatment. The expression of AdipoR2 tended (p = 0.09) to be higher in D7 lutea and was more prevalently localized to the cell surface of large and small luteal cells than in D5 tissue. Adiponectin influenced (p ≤ 0.05) FGF2, leptin, and AdipoR2 gene expression relative to the dose and day (D5 or D7). Collectively, the evidence supports the supposition that adiponectin influences angiogenic factors in the developing CL.

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