Lentivirus-mediated silencing of spindle and kinetochore-associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells

Shi, Xiujuan; Chen, Xianzhen; Hu, Peng; Song, E; Zhang, Ting; Zhang, Junxiang; Li, Jing; Swa, Himaya; Li, Yongxin; Kim, Se‐Kwon; Liu, Xiaoqing; Zhang, Chen

doi:10.3892/mmr.2015.3175

Cited by 13 publications

(11 citation statements)

References 26 publications

(40 reference statements)

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“…This was confirmed by the findings of Qin et al, which have shown that lentivirus-mediated siRNA against SKA1 inhibits HCC cell proliferation by inducing cell cycle arrest in the G0/G1 phase while promoting apoptosis [ 13 ]. Besides, SKA1 upregulation is significantly associated with the metastasis of NSCLC [ 23 ], and cell studies have revealed that knockdown of SKA1 expression by siRNA represses the invasion and metastasis of a range of cancer types, including renal cell carcinoma, salivary adenoid cystic carcinoma, prostate cancer, glioblastoma and NSCLC [ 23 , 29 , 31 , 33 ]. In addition, SKA1 has shown to be involved in the chemoresistance of cancer cells [ 23 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

SKA1 overexpression is associated with poor prognosis in hepatocellular carcinoma

et al. 2018

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BackgroundSKA1, an important mitosis protein, has been indicated in the initiation and progression of several malignancies. However, its clinical significance in hepatocellular carcinoma (HCC) remain to be elucidated.MethodsmRNA expression of SKA1 was examined in 126 HCC and paired non-neoplastic tissues using real-time PCR and validated in The Cancer Genome Atlas (TCGA) database. SKA1 protein expression was detected using immunohistochemistry in the 126 HCC tissues and its associations with clinicopathological parameters and prognosis were analyzed. Hierarchical cluster analysis and gene set enrichment analysis (GSEA) were performed in selected Gene Expression Omnibus data sets.ResultsSKA1 mRNA expression was significantly elevated in HCC tissues from both local hospital and TCGA database. Immunohistochemistry revealed that increased SKA1 expression was present in 65 of the 126 cases and was significantly associated with higher serum alpha-fetoprotein concentration, larger tumor size and higher TNM stage. Patients with positive SKA1 expression showed significantly worse overall and relapse-free survival. Multivariate Cox regression analysis revealed that SKA1 was an independent predictor of patient prognosis. Gene expression profiling analysis of public data showed that high-SKA1 expression HCC tissues had similar gene expression profiles with fetal liver tissues. Moreover, GSEA showed that genes up-regulated in high SKA1 HCC subgroup were significantly enriched in cell cycle pathway, while genes down-regulated were significantly enriched in apoptosis pathway.ConclusionsOur findings indicate that the oncofetal gene SKA1 might be involved in the progression of the HCC and could serve as a prognostic marker for HCC.Electronic supplementary materialThe online version of this article (10.1186/s12885-018-5119-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

SKA1 overexpression is associated with poor prognosis in hepatocellular carcinoma

et al. 2018

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“…Several studies have indicated that SKA1 is involved in the growth and proliferation of various types of cancer, including oral adenosquamous and hepatocellular carcinoma, bladder cancer, gastric cancer, prostate cancer, thyroid cancer, non-small cell lung cancer, and glioblastoma. (44)(45)(46)(47)(48)(49)(50)(51) In the human genome, SKA1, SKA2, and SKA3 form the SKA complex. During mitosis, the SKA complex is localized between the outer kinetochore interface and the spindle microtubules.…”

Section: Discussionmentioning

confidence: 99%

Regulation of spindle and kinetochore‐associated protein 1 by antitumor miR‐10a‐5p in renal cell carcinoma

et al. 2017

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Analysis of our original microRNA (miRNA) expression signature of patients with advanced renal cell carcinoma (RCC) showed that microRNA‐10a‐5p (miR‐10a‐5p) was significantly downregulated in RCC specimens. The aims of the present study were to investigate the antitumor roles of miR‐10a‐5p and the novel cancer networks regulated by this miRNA in RCC cells. Downregulation of miR‐10a‐5p was confirmed in RCC tissues and RCC tissues from patients treated with tyrosine kinase inhibitors (TKI). Ectopic expression of miR‐10a‐5p in RCC cell lines (786‐O and A498 cells) inhibited cancer cell migration and invasion. Spindle and kinetochore‐associated protein 1 (SKA1) was identified as an antitumor miR‐10a‐5p target by genome‐based approaches, and direct regulation was validated by luciferase reporter assays. Knockdown of SKA1 inhibited cancer cell migration and invasion in RCC cells. Overexpression of SKA1 was observed in RCC tissues and TKI‐treated RCC tissues. Moreover, analysis of The Cancer Genome Atlas database demonstrated that low expression of miR‐10a‐5p and high expression of SKA1 were significantly associated with overall survival in patients with RCC. These findings showed that downregulation of miR‐10a‐5p and overexpression of the SKA1 axis were highly involved in RCC pathogenesis and resistance to TKI treatment in RCC.

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“…Recently, a series of studies suggest the involvement of spindle and kinetochore-associated complex subunit 1 (SKA1) in the growth and proliferation of multiple cancer types, including oral adenosquamous ( 7 ) and hepatocellular carcinoma ( 8 ), bladder ( 9 ), gastric ( 10 ), prostate ( 11 ) and thyroid cancer ( 12 ), and glioblastoma ( 13 ). SKA1, along with SKA2 and SKA3, constitute the SKA complex.…”

Section: Introductionmentioning

confidence: 99%

SKA1 regulates the metastasis and cisplatin resistance of non-small cell lung cancer

et al. 2016

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Currently, chemotherapy with platinum-based drugs including cisplatin is the most effective therapy for the treatment of non-small cell lung carcinoma (NSCLC). However, the efficacy of chemotherapy is limited due to commonly developed drug resistance. Spindle and kinetochore-associated complex subunit 1 (SKA1) is part of a complex essential for stabilizing the attachment of spindle microtubules to kinetochores and for maintaining the metaphase plate during mitosis. In the present study, we aimed to investigate the role of SKA1 in the process of metastasis and drug resistance of NSCLC. We completed a series of experiments to investigate the function of SKA1 in NSCLC metastasis and drug resistance including qRT-PCR, immunohistochemistry and western blotting, as well as MTT, BrdU, wounded healing, Transwell and gelatin zymography assays. We demonstrated that the expression levels of SKA1 were elevated in NSCLC and were correlated with cancer progression and malignancy. We also reported that SKA1 positively regulated the proliferation and metastatic ability of NSCLC cells. In addition, we determined that SKA1 contributed to cisplatin resistance in NSCLC cells by protecting these cells from cisplatin-induced cell apoptosis. SKA1 also appeared to regulate the ERK1/2 and the Akt-mediated signaling pathways in NSCLC cells. SKA1 is required for metastasis and cisplatin resistance of non-small cell lung cancer.

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Lentivirus-mediated silencing of spindle and kinetochore-associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells

Cited by 13 publications

References 26 publications

SKA1 overexpression is associated with poor prognosis in hepatocellular carcinoma

SKA1 overexpression is associated with poor prognosis in hepatocellular carcinoma

Regulation of spindle and kinetochore‐associated protein 1 by antitumor miR‐10a‐5p in renal cell carcinoma

SKA1 regulates the metastasis and cisplatin resistance of non-small cell lung cancer

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