Lentivirus genomic organization: The complete nucleotide sequence of the env gene region of equine infectious anemia virus

Rushlow, K E; Olsen, Ken; Stiegler, Gary L.; Payne, Susan; Montelaro, Ronald C.; Issel, Charles J.

doi:10.1016/0042-6822(86)90195-9

Cited by 109 publications

(109 citation statements)

References 49 publications

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…13. Equine infectious anaemia virus (genus Lentivirus), residues 446 to 535, Rushlow et al (1986). 14, Human spumavirus (genus Spumavirus), residues 569 to 694, Flugel et aL (1987).…”

mentioning

confidence: 99%

Heptad Repeat Sequences are Located Adjacent to Hydrophobic Regions in Several Types of Virus Fusion Glycoproteins

Chambers

Pringle²,

Easton³

1990

Journal of General Virology

269

212

View full text Add to dashboard Cite

“…13. Equine infectious anaemia virus (genus Lentivirus), residues 446 to 535, Rushlow et al (1986). 14, Human spumavirus (genus Spumavirus), residues 569 to 694, Flugel et aL (1987).…”

mentioning

confidence: 99%

Heptad Repeat Sequences are Located Adjacent to Hydrophobic Regions in Several Types of Virus Fusion Glycoproteins

Chambers

Pringle²,

Easton³

1990

Journal of General Virology

269

212

View full text Add to dashboard Cite

“…However, the results presented here cannot completely exclude the possibility that the horse serum recognizes non-linear protein determinants on gpl20 that are held in place by the presence of oligosaccharide chains. In support of the first model is the fact that EIAV gp90 and HIV gpl20 lack significant env sequence homology, even though they display remarkably similar structural features (Rushlow et al, 1986). The latter model may be supported by the observation that HIV gpl20 reactivity with AIDS patient sera is significantly altered by changes in glycoprotein conformation, as observed when comparing non-denaturing and denaturing conditions, and reducing and non-reducing assay conditions (W. G. Robey, unpublished data).…”

mentioning

confidence: 76%

Characterization of the Serological Cross-reactivity between Glycoproteins of the Human Immunodeficiency Virus and Equine Infectious Anaemia Virus

Montelaro

Robey²,

West³

et al. 1988

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYThe reported serological relatedness between the major glycoproteins of human immunodeficiency virus (HIV gpl20) and equine infectious anaemia virus (EIAV gp90) was examined using purified antigens in radioimmunoprecipitation (RIP), radioimmunoassay (RIA) and immunoblot assays with reference serum from acquired immunodeficiency syndrome (AIDS) patients, an anti-gpl20 goat serum and EIAVinfected horse serum. To assess the contributions of glycoprotein oligosaccharide and peptide components to any observed reactivities, antigens treated with endoglycosidase F to remove carbohydrate were assayed in parallel with the intact glycoprotein. The results of the experiments indicated that the reactivity observed for each antigen was dependent on the immunoassay employed. The RIP and RIA analyses demonstrated that HIV gp 120 is equally reactive with the AIDS patient serum, the goat anti-gp 120 serum and the EIAV-infected horse serum, whereas the EIAV gp90 reacted only with the horse serum. In immunoblot assays, the HIV gpl20 reacted with AIDS patient serum, but not with the EIAV-infected horse serum. Deglycosylation of the HIV gpl20 evidently increased its reactivity with the AIDS patient serum, had no significant effect on its reactivity with the goat antiserum, and essentially abolished its reactivity with the EIAV reference serum. Thus, it appears that the serological crossreactivity observed between HIV gpl20 and sera from EIAV-infected horses can be attributed to the oligosaccharide rather than the peptide components of the viral glycoprotein. These studies also emphasize the necessity of employing several assay procedures in assessing lentivirus antigenicity.

show abstract

“…In addition, the lentiviruses have unique small open reading frames (ORFs) located between pol and env and at the 3' terminus Davis et al, 1987;Sonigo et al, 1985). In HIV, visna virus and EIAV, these ORFs code for regulatory proteins (Rather et al, 1985;Sanchez-Pescador et al, 1985;Wain-Hobson et al, 1985;Rushlow et al, 1986;Derse et al, 1987). At both the 5' and 3' ends of the RNA (R region) are sequences that contain the cap site, the polyadenylation signal and the termination signal for viral RNA transcription (Hess et al, 1986).…”

Section: Genetic Structure and Replicationmentioning

confidence: 99%

Biology and Pathogenesis of Lentiviruses

Narayan¹,

Clements

1989

Journal of General Virology

243

148

View full text Add to dashboard Cite

Lentivirus genomic organization: The complete nucleotide sequence of the env gene region of equine infectious anemia virus

Cited by 109 publications

References 49 publications

Heptad Repeat Sequences are Located Adjacent to Hydrophobic Regions in Several Types of Virus Fusion Glycoproteins

Heptad Repeat Sequences are Located Adjacent to Hydrophobic Regions in Several Types of Virus Fusion Glycoproteins

Characterization of the Serological Cross-reactivity between Glycoproteins of the Human Immunodeficiency Virus and Equine Infectious Anaemia Virus

Biology and Pathogenesis of Lentiviruses

Contact Info

Product

Resources

About