Lentinan modulates intestinal microbiota and enhances barrier integrity in a piglet model challenged with lipopolysaccharide

Wang, Xiuying; Wang, Wenjun; Wang, Longmei; Yu, Chao; Zhang, Guolong; Zhu, Huiling; Wang, Chunwei; Zhao, Shengjun; Hu, Chien‐An Andy; Liu, Yulan

doi:10.1039/c8fo02438c

Cited by 70 publications

(55 citation statements)

References 40 publications

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“…The destructed villus morphology and inflammatory reaction were induced by LPS injection, which decreased the growth performance of chicks. These phenotypes indicated that intestinal injury model had been successfully established, which is consistent with the previous researches ( Wang et al., 2019b , Zhuang et al., 2019 ). In line with our expectations, genistein treatment alleviated LPS-induced intestinal damage and improved growth performance of chicks.…”

Section: Discussionsupporting

confidence: 90%

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Dai

Song

et al. 2020

Poultry Science

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Genistein is abundant in the corn-soybean meal feed. Little information is available about the effect of dietary genistein on the intestinal transcriptome of chicks, especially when suffering from intestinal injury. In this study, 180 one-day-old male ROSS 308 broiler chickens were randomly allocated to 3 groups, with 4 replicates (cages) of 15 birds each. The treatments were as follows: chicks received a basal diet (CON), a basal diet and underwent lipopolysaccharide-challenge (LPS), or a basal diet supplemented with 40 mg/kg genistein and underwent LPS-challenge (GEN). LPS injection induced intestinal injury and inflammatory reactions in the chicks. Transcriptomic analysis identified 7,131 differently expressed genes (3,281 upregulated and 3,851 downregulated) in the GEN group compared with the LPS group ( P adjusted value < 0.05, |fold change| > 1.5), which revealed that dietary genistein exposure altered the gene expression profile and signaling pathways in the ileum of LPS-treated chicks. Furthermore, dietary genistein improved intestinal morphology, mucosal immune function, tight junction, antioxidant activity, apoptotic process, and growth performance, which were adversely damaged by LPS injection. Therefore, adding genistein into the diet of chicks can alter RNA expression profile and ameliorate intestinal injury in LPS-challenged chicks, thereby improving the growth performance of chicks with intestinal injury.

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Section: Discussionsupporting

confidence: 90%

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Dai

Song

et al. 2020

Poultry Science

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“…The concentrations of SCFA in caecal and colonic digesta were analysed as previously described (14) using GC (HP 6890 Series, Hewlett Packard) equipped with a HP 19091N-213 column with The V3-V4 region of the bacteria 16S rRNA gene was amplified by PCR using 338F/806R barcoded primers. PCR amplification products were quantified with a QuantiFluor TM -ST Handheld Fluorometer with UV/Blue Channels (Promega Corporation), normalised and then sequenced on Miseq (Illumina).…”

Section: Scfa Analysismentioning

confidence: 99%

Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs

Xu¹,

Hua²,

Wang³

et al. 2020

Br J Nutr

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The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury. A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge. After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma. Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets. Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin. In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge. These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.

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“…The increased ratio of Bacteroidetes to Firmicutes is responsible for protecting the host against systemic inammatory diseases. 57 The Bacteroidetes to Firmicutes ratio in the LPS + WPSPP-1 group was increased as compared with that of the LPS model group. The abnormal dilatation of Proteobacteria is generally considered to be a key feature of the intestinal microbiota imbalance.…”

Section: Diversity Of Gut Microbiota In Lps-induced Inammatory Micementioning

confidence: 86%

Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato

et al. 2020

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Lentinan modulates intestinal microbiota and enhances barrier integrity in a piglet model challenged with lipopolysaccharide

Abstract: The protective effects of lentinan may be associated with inhibition of inflammation, production of SCFAs, and alterations of microbiota composition.

Cited by 70 publications

References 40 publications

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Dietary genistein supplementation protects against lipopolysaccharide-induced intestinal injury through altering transcriptomic profile

Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs

Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato

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