Lentinan in-situ coated tungsten oxide nanorods as a nanotherapeutic agent for low power density photothermal cancer therapy

Tian, Yilong; Yi, Wenhui; Bai, Lei; Zhang, Ping; Si, Jinhai; Hou, Xun; Deng, Yuanyuan; Hou, Jin

doi:10.1016/j.ijbiomac.2019.06.183

Cited by 34 publications

(17 citation statements)

References 43 publications

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“…In previous studies, the clinical value of traditional Chinese medicines have been assessed, due to the lower incidence of side effects ( 2 , 3 ). Increasing attention has been given to a fungal polysaccharide, lentinan (LNT), due to its strong antitumor activity ( 4 , 5 ). It was reported to effectively inhibit proliferation, differentiation, growth and senescence of cells ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

“…It was reported to effectively inhibit proliferation, differentiation, growth and senescence of cells ( 6 ). LNT has also been reported to effectively prevent development of cancers caused by chemical or viral carcinogens ( 4 , 5 ). Clinically, LNT enhanced the effects chemotherapy and improved the survival of patients with several types of cancer, including gastric, colon, breast and lung cancer ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

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Oridonin improves the therapeutic effect of lentinan on lung cancer

2021

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Oridonin, a compound from Rabdosia rubescens , has been shown to exhibit a potent ability to improve the antitumor effects of lentinan (LNT). In the present study, the effects of oridonin, LNT, and the combination of these treatments were assessed on the normal human fetal lung fibroblast cell line MRC-5, as well as the non-small cell lung cancer cell line A549. Next, their effects on metastasis and survival in vivo were assessed in a mouse model of lung cancer. The effects of the treatments on the mRNA and protein expression levels of several regulatory factors in A549 cells and lung tissues were determined using reverse transcription-quantitative PCR and western blotting. The results showed that the viability of MRC-5 and A549 cells were not affected by 0-20 µg/ml oridonin; 0-300 µg/ml LNT did not affect the viability of MRC-5 cells, but 50-400 µg/ml LNT reduced the viability of A549 cells. Thus, 20 µg/ml oridonin and 100 or 300 µg/ml LNT were used in the subsequent experiments. Treatment with oridonin and LNT, alone or combined, had no effect on MRC-5 cell viability. Oridonin treatment had no effect on A549 cell viability; however, LNT suppressed A549 cell viability, and oridonin promoted the suppressive effects of LNT on A549 cells. In vivo analysis showed that oridonin alone had no effect on metastasis and survival, but LNT decreased metastasis and survival in mice. Oridonin augmented the effects of LNT against metastasis and further improved the survival rates of mice. In both A549 cells and lung tissues, LNT increased the mRNA and protein expression levels of caspase-3, caspase-8, caspase-9, Bax, p53, p21 and inhibitor of nuclear factor-κB (NF-κB)-α, and reduced the mRNA and protein expression levels of Bcl-2 and NF-κB. Oridonin augmented all the effects of LNT on expression of these proteins in the cells. Together, the results showed that oridonin enhanced the antitumor effects of LNT, and may thus serve as an adjuvant alongside LNT as a novel anticancer regimen for treatment of lung cancer.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oridonin improves the therapeutic effect of lentinan on lung cancer

2021

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“…In recent years, traditional medicine has been studied for clinical treatment for their low side effects (2,3). To achieve fewer side effects, increasing attention has been given to a fungal polysaccharide, lentinan (LNT), for its strong antitumor activity (4,5). It was reported to effectively affect division, differentiation, growth, and senescence of cells (6).…”

Section: Introductionmentioning

confidence: 99%

Oridonin Improves the Therapeutic Effect of Lentinan on Lung Cancer

Gui

Cheng

Chen

2021

Preprint

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Oridonin, a compound from Rabdosia rubescens, has been shown to have a potency for the improvement of the antitumor effect of lentinan (LNT). In this study, we tested the effect of oridonin, LNT, and the combination of them on a normal human fetal lung fibroblast cell line MRC-5 and non-small cell lung cancer cell line A549. Then we tested their effects on metastasis and survival with a lung cancer mice model. The effects of them on the mRNA and protein expression of several regulatory factors in A549 and lung tissue were determined by QPCR and western blotting. Results showed that the viability of MRC-5 and A549 were not affected by 0-20 µg/ml oridonin. 0-300 µg/ml LNT did not affect the viability of MRC-5, but 50-400 µg/ml LNT inhibited the viability of A549. 20 µg/ml oridonin and 100 or 300 µg/ml LNT were used in the subsequent study. The oridonin, LNT, or the combination of both had no effect on MRC-5 cell viability. The oridonin had no effect on A549 cell viability but LNT suppressed A549 cell viability, and oridonin promoted the suppression of LNT on A549 cells. In vivo study showed that oridonin alone had no effect on metastasis and survival but LNT decreased metastasis and survival in mice. Oridonin improved the suppression of LNT against metastasis and further improved the survival rate. In both A549 and lung tissues, LNT increased the mRNA and protein expression of caspase-3, caspase-8, caspase-9, Bax, p53, p21, and IκB-α, reduced mRNA and protein expressions of Bcl-2 and NF-κB. Oridonin enhanced all the effects of LNT on cells. Our study demonstrated that oridonin enhanced the antitumor effects of LNT and is conducive to the development of oridonin and LNT as a novel cancer drug regimen.

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“…To achieve fewer side effects, increasing attention has been given to a fungal polysaccharide, lentinan (LNT), for its strong antitumor activity (3,4). It was reported to effectively affect division, differentiation, growth, and senescence of cells (5).…”

Section: Introductionmentioning

confidence: 99%