Lentil Breeding in Genomic Era: Present Status and Future Prospects

Kumar, Jitendra; Gupta, Debjyoti Sen; Tiwari, Pravin

doi:10.1007/978-3-030-47306-8_7

Cited by 4 publications

(4 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As for most cool season grain legumes [ 8 , 27 ], the phenotypic expression of the rust resistance reactions reported so far in lentils is poorly described. Rust resistance breeding in lentil, as in most cool season legumes, has been hampered by the relatively low investment in genetics, genomics and biotechnology of the legume crops which is impressively improving recently [ 34 ]. However, less attention has been paid to the understanding of the rust pathogen, with still little agreement on its host specialization and the existence of races [ 5 , 8 ], contrasting with the situation of rusts of other legumes such as common bean ( Phaseolus vulgaris ) and soybean ( Glycine max ) which have been largely studied leading to the identification of races and of resistance genes [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives

Barilli

Rubiales

2023

Plants

View full text Add to dashboard Cite

Lentil rust is a major disease worldwide caused by Uromyces viciae-fabae. In this study, we screened a large germplasm collection of cultivated lentils (Lens culinaris ssp. culinaris) and its wild relatives, both in adult plants in the field with a local rust isolate during 2 seasons and in seedlings under controlled conditions with four fungal isolates of worldwide origin. The main results from our study were the following: (1) a significant number of accessions with resistance based on hypersensitive reaction (reduced Infection Type (IT)) were identified in cultivated lentil and in L. ervoides, L. nigricans and L.c. orientalis. The IT scores showed a clear isolate-specific response suggesting race-specificity, so each fungal isolate might be considered a different race. Resistance was identified against all isolates what might be the basis to develop a standard differential set that should be a priority for rust definition and monitoring. (2) Interestingly, although at lower frequency than in L. ervoides and L. nigricans, the hypersensitive response was also observed within cultivated lentil, with accession 1561 (L.c. culinaris) displaying resistance to the four isolates making this accession a valuable ready-to-use resource for lentil resistance breeding. Resistance to all other rust isolates was also available within L.c. culinaris in an isolate-specific manner. Accession 1308 (L. ervoides) showed resistance against all isolates tested, as well as a reduced number of accessions belonging to other wild Lens species. (3) In addition, our screenings allowed the identification of several accessions with partial resistance (reduced Disease Severity (DS) despite high IT). Adult Plant Resistance resulting in reduced severity in adult plants in the field, despite high susceptibility in seedlings, was more frequently identified in L.c. culinaris, but also in L. nigricans and L.c. orientalis.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives

Barilli

Rubiales

2023

Plants

View full text Add to dashboard Cite

show abstract

“…However, limited efforts have been made toward the implementation of genomics-assisted breeding in lentils. Between 2010 and 2020, advancements in next-generation sequencing (NGS) technologies have led to accelerated development of genomic resources in lentils ( Kumar et al, 2015 , 2020a ). The advent of novel genomic information resulted in the identification of several molecular markers, broadly classified into three classes, i.e., hybridization-based, PCR-based, and NGS-based markers.…”

Section: Genomic Innovations Reveal a New Genetic Landscape For Lenti...mentioning

confidence: 99%

“…Besides quenching micronutrient deficiency, it is recommended to patients suffering from diabetes, obesity, and cardiovascular diseases because of its low glycemic index ( Srivastava and Vasishtha, 2012 ). Moreover, the cultivation of lentils provides various crop rotational benefits to cereal crops, including symbiotic nitrogen fixation, carbon sequestration, and controlling the proliferation of pests ( Kumar et al, 2014 , 2020a ). Lentils beneficial role in terms of nutrition and ecological sustainability has raised its demand, translating to an increased global lentil production from 0.85 to 6.53 Mt over the past six decades ( FAOSTAT, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Omics Path to Increasing Productivity in Less-Studied Crops Under Changing Climate—Lentil a Case Study

et al. 2022

View full text Add to dashboard Cite

Conventional breeding techniques for crop improvement have reached their full potential, and hence, alternative routes are required to ensure a sustained genetic gain in lentils. Although high-throughput omics technologies have been effectively employed in major crops, less-studied crops such as lentils have primarily relied on conventional breeding. Application of genomics and transcriptomics in lentils has resulted in linkage maps and identification of QTLs and candidate genes related to agronomically relevant traits and biotic and abiotic stress tolerance. Next-generation sequencing (NGS) complemented with high-throughput phenotyping (HTP) technologies is shown to provide new opportunities to identify genomic regions and marker-trait associations to increase lentil breeding efficiency. Recent introduction of image-based phenotyping has facilitated to discern lentil responses undergoing biotic and abiotic stresses. In lentil, proteomics has been performed using conventional methods such as 2-D gel electrophoresis, leading to the identification of seed-specific proteome. Metabolomic studies have led to identifying key metabolites that help differentiate genotypic responses to drought and salinity stresses. Independent analysis of differentially expressed genes from publicly available transcriptomic studies in lentils identified 329 common transcripts between heat and biotic stresses. Similarly, 19 metabolites were common across legumes, while 31 were common in genotypes exposed to drought and salinity stress. These common but differentially expressed genes/proteins/metabolites provide the starting point for developing high-yielding multi-stress-tolerant lentils. Finally, the review summarizes the current findings from omic studies in lentils and provides directions for integrating these findings into a systems approach to increase lentil productivity and enhance resilience to biotic and abiotic stresses under changing climate.

show abstract

“…Of these, ascochyta blight, botrytis grey mould, boron and salinity have been identified as key limiting factors across a wide range of production regions ( Davidson et al., 2016 ; Sudheesh et al., 2016 ; Rodda et al., 2017 ; Rodda et al., 2018 ; Dissanayake et al., 2020 ; Kumar et al., 2021 ) that can result in yield losses of 30-40% ( Kumar et al., 2013 ). Climate variability and complex genotype-by-environment (G × E) interactions on the expression of phenotypic traits also contribute to low genetic gain in lentil breeding ( Kumar and Ali, 2006 ; Kumar et al., 2020 ). As more than 95% of the Australian lentil crop is exported, grain quality traits such as grain weight, seed size and protein content are also important in meeting market demands.…”

Section: Introductionmentioning

confidence: 99%

Genomic selection for target traits in the Australian lentil breeding program

Gebremedhin,

Li,

Shunmugam

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Genomic selection (GS) uses associations between markers and phenotypes to predict the breeding values of individuals. It can be applied early in the breeding cycle to reduce the cross-to-cross generation interval and thereby increase genetic gain per unit of time. The development of cost-effective, high-throughput genotyping platforms has revolutionized plant breeding programs by enabling the implementation of GS at the scale required to achieve impact. As a result, GS is becoming routine in plant breeding, even in minor crops such as pulses. Here we examined 2,081 breeding lines from Agriculture Victoria’s national lentil breeding program for a range of target traits including grain yield, ascochyta blight resistance, botrytis grey mould resistance, salinity and boron stress tolerance, 100-grain weight, seed size index and protein content. A broad range of narrow-sense heritabilities was observed across these traits (0.24-0.66). Genomic prediction models were developed based on 64,781 genome-wide SNPs using Bayesian methodology and genomic estimated breeding values (GEBVs) were calculated. Forward cross-validation was applied to examine the prediction accuracy of GS for these targeted traits. The accuracy of GEBVs was consistently higher (0.34-0.83) than BLUP estimated breeding values (EBVs) (0.22-0.54), indicating a higher expected rate of genetic gain with GS. GS-led parental selection using early generation breeding materials also resulted in higher genetic gain compared to BLUP-based selection performed using later generation breeding lines. Our results show that implementing GS in lentil breeding will fast track the development of high-yielding cultivars with increased resistance to biotic and abiotic stresses, as well as improved seed quality traits.

show abstract

Lentil Breeding in Genomic Era: Present Status and Future Prospects

Cited by 4 publications

References 93 publications

Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives

Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives

Omics Path to Increasing Productivity in Less-Studied Crops Under Changing Climate—Lentil a Case Study

Genomic selection for target traits in the Australian lentil breeding program

Contact Info

Product

Resources

About