Lensfree microscopy on a cellphone

Abstract

We demonstrate lensfree digital microscopy on a cellphone. This compact and light-weight holographic microscope installed on a cellphone does not utilize any lenses, lasers or other bulky optical components and it may offer a cost-effective tool for telemedicine applications to address various global health challenges. Weighing ~38 grams (<1.4 ounces), this lensfree imaging platform can be mechanically attached to the camera unit of a cellphone where the samples are loaded from the side, and are vertically illuminated by a simple light-emitting diode (LED). This incoherent LED light is then scattered from each micro-object to coherently interfere with the background light, creating the lensfree hologram of each object on the detector array of the cellphone. These holographic signatures captured by the cellphone permit reconstruction of microscopic images of the objects through rapid digital processing. We report the performance of this lensfree cellphone microscope by imaging various sized micro-particles, as well as red blood cells, white blood cells, platelets and a waterborne parasite (Giardia lamblia).

Single-pixel digital ghost holography

“…Taking biological applications as an example, digital holographic microscopy offers the capability of measuring phase variations in the nanometer range, allowing a marker-free quantitative analysis in the cellular and subcellular ranges [6]. Nowadays, digital holographic microscopes are so compact and versatile that they can be integrated in a cellular phone to offer a cost-effective tool for telemedicine applications [7].…”

Handheld high-throughput plasmonic biosensor using computational on-chip imaging

“…Similarly, various point-of-care diagnostic devices have been developed and among them optical imaging and sensing techniques are highly advantageous as they can provide real-time, highresolution and highly sensitive quantitative information, potentially assisting rapid and accurate diagnosis. [30][31][32][33][34][35][36][37][38][39][40] To date, a number of optical techniques have been proposed for point-of-care diagnostics such as in vitro optical devices, [41][42][43][44][45][46][47][48][49][50][51][52][53] including portable optical imaging systems, optical microscopes integrated to cell phones or in vivo optical devices, [54][55][56][57][58][59][60][61][62][63] involving confocal microscopy, microendoscopy and optical coherence tomography techniques. Among these approaches, lens-free computational on-chip imaging 64 has been an emerging technique that can eliminate the need for bulky and costly optical components while also preserving (or even enhancing in certain cases) the image resolution, field of view and sensitivity.…”

Шляхи Розвитку Мобільної Медицини

“…(лікарі в навчальних закладах, медичні сестри, суміжні медичні працівники) можуть забезпечити виконання простих завдань з отримання та аналізу зображень [7,16]. Це визначає значні вигоди в сіль-ських і віддалених районах, де є дистрибутивні проблеми та брак спеціалізованих кадрів.…”