Lens development

McAvoy, John W.; Chamberlain, Coral G.; Longh, R U de; Hales, Angela M.; Lovicu, Frank J.

doi:10.1038/eye.1999.117

Cited by 231 publications

(178 citation statements)

References 56 publications

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“…3B,C). At E12.5, strong staining was observed in both the posterior wall, containing elongating primary fibers and epithelial cells at the anterior pole, which remain undifferentiated and retain their proliferation capacity (McAvoy et al, 1999;Menko, 2002;Lovicu and McAvoy, 2005).…”

Section: Spatial Segregation Of Gad Forms During Lens Developmentmentioning

confidence: 99%

“…Ocular lens morphogenesis proceeds through highly regulated developmental stages during which proliferating epithelial cells near the lens equator continuously differentiate into fiber cells that elongate and move inward being replaced by newly differentiating fibers (McAvoy et al, 1999). The progress from epithelial to terminally differentiated fiber cells proceeds through well-defined stages characterized by the synthesis of specific sets of intermediate filament proteins (Blankenship et al, 2001), adhesion molecules (Beebe et al, 2001;Zelenka, 2004), and lens crystallins (␣, ␤, ␥, ␦).…”

Section: Sequential Induction and Spatial Segregation Of Different Gamentioning

confidence: 99%

“…The lens derives from a thickening of the surface ectoderm, the lens placode, at around embryonic day (E) 9.5 (for recent review on lens development, refer to Piatigorsky, 1981;McAvoy et al, 1999;Menko, 2002;Lovicu and McAvoy, 2005). As differentiation proceeds, the presumptive lens ectoderm invaginates to first form the lens cup (lens pit) at E10.5 and subsequently the lens vesicle (at E11.5), which is composed of a single layer of epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

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GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens: Correlation with Dlx2 and Dlx5

Kwakowsky

Schwirtlich

Zhang

et al. 2007

Developmental Dynamics

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Gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter of the adult nervous system and its biosynthetic enzyme glutamic acid decarboxylase (GAD) are abundantly expressed in the embryonic nervous system and are involved in the modulation of cell proliferation, migration, and differentiation. Here we describe for the first time the expression of GABA and embryonic and adult GAD isoforms in the developing mouse lens. We show that the GAD isoforms are sequentially induced with specific spatiotemporal profiles: GAD65 and embryonic GAD isoforms prevail in primary fibers, while GAD67 is the predominant GAD expressed in the postnatal secondary fibers. This pattern correlates well with the expression of Dlx2 and Dlx5, known as upstream regulators of GAD. GABA and GAD are most abundant at the tips of elongating fibers and are absent from organelle-free cells, suggesting their involvement is primarily in shaping of the cytoskeleton during fiber elongation stages. Developmental Dynamics 236: 3532-3544, 2007.

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Section: Spatial Segregation Of Gad Forms During Lens Developmentmentioning

confidence: 99%

Section: Sequential Induction and Spatial Segregation Of Different Gamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens: Correlation with Dlx2 and Dlx5

Kwakowsky

Schwirtlich

Zhang

et al. 2007

Developmental Dynamics

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“…Progeny of these divisions are displaced posteriorly, into the transitional zone, where they begin to differentiate into fibre cells. Fibre differentiation is characterised by distinct molecular and morphologic changes such as exit from the cell cycle, cell elongation, loss of cytoplasmic organelles and nuclei, as well as the accumulation of fibre-specific crystallin proteins [1,2,3]. The normal architecture, and hence transparency of the lens, depends on the maintenance of this distinctive pattern of growth.…”

Section: Introductionmentioning

confidence: 99%

Sef and Sprouty expression in the developing ocular lens: Implications for regulating lens cell proliferation and differentiation

Boros

Newitt

Wang

et al. 2006

Seminars in Cell & Developmental Biology

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In many developmental systems, growth factor signalling must be temporally and spatially regulated, and this is commonly achieved by growth factor antagonists. Here we describe the expression patterns of newly identified growth factor inhibitors, Sprouty and Sef, in the developing ocular lens. Sprouty and Sef are both expressed in the lens throughout embryogenesis, and become restricted to the lens epithelium, indicating that lens cell proliferation and fibre differentiation may be tightly regulated by such antagonists. Future studies will be aimed at understanding how these negative regulatory molecules modulate growth factor-induced signalling pathways and cellular processes in the lens.

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“…Cell propagation and transformation via the equatorial zone contribute to the formation and growth of precursors of secondary lens fiber cells. At a later developmental stage, the transparency of these fiber cells results from denucleation and degradation of their organelles through programmed cell death (reviewed in Wride, 1996;Dahm, 1999;McAvoy et al, 1999;Bassnett, 2002).…”

Section: Introductionmentioning

confidence: 99%

Recapitulation of human βB1‐crystallin promoter activity in transgenic zebrafish

Hou

Kuo

Luo

et al. 2005

Developmental Dynamics

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Development of the eye is morphologically similar among vertebrates, indicating that the underlying mechanism regulating the process may have been highly conserved during evolution. Herein we analyzed the promoter of the human ␤B1-crytallin gene in zebrafish by transgenic experiments. To delineate the evolutionarily conserved regulatory elements, we performed serial deletion assays in the promoter region. The results demonstrated that the ؊90/؉61-bp upstream proximal promoter region is sufficient to confer lens-tissue specificity to the human ␤B1-crystallin gene in transgenic zebrafish. Through phylogenetic sequence comparisons and an electrophoretic mobility shift assay (EMSA), a highly conserved cis-element of a six-base pair sequence TG(A/C)TGA, the consensus sequence for the Maf protein binding site, within the proximal promoter region was revealed. Further, a site-mutational assay showed that this element is crucial for promoter activity. These data suggest that the fundamental transcriptional regulatory mechanism of the ␤B1-crystallin gene has been well conserved between humans and zebrafish, and plausibly among all vertebrates, during evolution. Developmental Dynamics 235:435-443, 2006.

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Lens development

Cited by 231 publications

References 56 publications

GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens: Correlation with Dlx2 and Dlx5

GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens: Correlation with Dlx2 and Dlx5

Sef and Sprouty expression in the developing ocular lens: Implications for regulating lens cell proliferation and differentiation

Recapitulation of human βB1‐crystallin promoter activity in transgenic zebrafish

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