Lens cells: More than meets the eye

Tholozan, F.; Quinlan, Roy A.

doi:10.1016/j.biocel.2007.06.021

Cited by 32 publications

(20 citation statements)

References 17 publications

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“…During lens development, the progeny of epithelial cells migrate into the transitional zone and elongate and differentiate into lens fiber cells (18,31). Timely differentiation and correct migration of lens fibers are crucial for continuous addition of fiber mass and formation of a correctly organized lens.…”

Section: Mir-204 Controls Lens Differentiation By Targeting Meis2 Andmentioning

confidence: 99%

miR-204 is required for lens and retinal development via Meis2 targeting

Conte

Carrella²,

Avellino³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

149

176

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MicroRNAs (miRNAs) are small noncoding RNAs that have important roles in the regulation of gene expression. The roles of individual miRNAs in controlling vertebrate eye development remain, however, largely unexplored. Here, we show that a single miRNA, miR-204, regulates multiple aspects of eye development in the medaka fish (Oryzias latipes). Morpholino-mediated ablation of miR-204 expression resulted in an eye phenotype characterized by microphthalmia, abnormal lens formation, and altered dorsoventral (D-V) patterning of the retina, which is associated with optic fissure coloboma. Using a variety of in vivo and in vitro approaches, we identified the transcription factor Meis2 as one of the main targets of miR-204 function. We show that, together with altered regulation of the Pax6 pathway, the abnormally elevated levels of Meis2 resulting from miR-204 inactivation are largely responsible for the observed phenotype. These data provide an example of how a specific miRNA can regulate multiple events in eye formation; at the same time, they uncover an as yet unreported function of Meis2 in the specification of D-V patterning of the retina.microRNA | eye development | medaka fish

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Section: Mir-204 Controls Lens Differentiation By Targeting Meis2 Andmentioning

confidence: 99%

miR-204 is required for lens and retinal development via Meis2 targeting

Conte

Carrella²,

Avellino³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

149

176

View full text Add to dashboard Cite

show abstract

“…The central lens epithelium, facing the anterior chamber of the eye and bathed in the aqueous humor, is a monolayer of epithelial cells where cells proliferate (Tholozan and Quinlan 2007). In the present study, the seven members of ADAM family ADAM9, ADAM10, ADAM12, ADAM13, ADAM17, ADAM22 and ADAM23 all are expressed by the cells in the CE, and as embryos develop, the expression of the individual ADAM decreases gradually (Figs.…”

Section: Expression Of Adams In the Central Lens Epithelium And Cell mentioning

confidence: 78%

“…During both embryonic development and adulthood the anterior epithelium provides a continuous source of undifferentiated cells that migrate toward the equator region of the lens, where the cells start differentiation into fiber cells (Leong et al 2000;Tholozan and Quinlan 2007).…”

Section: Expression Of Adams In the Lens Epithelium And Cells Migrationmentioning

confidence: 99%

“…It is composed of two contiguous cell subpopulations with very different morphology: (1) the epithelial cells facing the anterior chamber of the eye and (2) the fiber cells in the posterior part of the lens. Fiber cells are continually produced throughout whole life (Lovicu and McAvoy 2005;Tholozan and Quinlan 2007). The embryonic development of the lens is characterized by the processes of cellular proliferation, migration and differentiation.…”

Section: Introductionmentioning

confidence: 99%

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Expression patterns of ADAMs in the developing chicken lens

Yan¹,

Lin

Rolfs³

et al. 2012

J Mol Hist

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In the present study the expression patterns of ADAM (a disintegrin and metalloprotease) genes in the chicken developing lens were analyzed. Using in situ hybridization, we found that seven members of the ADAM family including ADAM9, ADAM10, ADAM12, ADAM13, ADAM17, ADAM22, and ADAM23 are expressed in the developing embryonic lens. From embryonic incubation day (E) 2 to E3, most of the ADAMs investigated here are expressed in the lens placode and lens vesicle. From E5 to E7, all seven ADAMs, but predominantly ADAM9 and ADAM10, are throughly expressed in the central epithelium, as well as in the proliferating lens epithelium and the equatorial lens epithelium. From E9 to E14, expression of ADAM9, ADAM10, and ADAM17 decreases moderately in these regions. ADAM12 and ADAM13 are weakly expressed in the central epithelium and the lens epithelium, and are not detectable from E14 onward. ADAM22 and ADAM23 are expressed in the central epithelium, the lens epithelium and the equatorial lens epithelium at E5 and decrease gradually afterwards in the same regions. At E16, only weak ADAM9, ADAM10 and ADAM17 signals are found in the anterior lens epithelium. The changing spatiotemporal expression of the seven ADAMs suggests a regulatory role for these molecules during chicken lens development.

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“…Mitotically active epithelial cells at the lens equator differentiate into the lens fiber cells, which lose major intracellular organelles and accumulate high concentrations of soluble crystallins (1). The loss of cellular organelles makes the fiber cells become incapable of supporting an active cellular metabolism.…”

mentioning

confidence: 99%

Amino Acid Residue Val362 Plays a Critical Role in Maintaining the Structure of C Terminus of Connexin 50 and in Lens Epithelial-fiber Differentiation

Shi

Banks

et al. 2010

Journal of Biological Chemistry

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We have previously shown that connexin (Cx) 50, unlike the other two lens connexins, Cx43 and Cx46, promotes chicken lens epithelial-fiber differentiation in a channel-independent manner. Here, we show that deletion of the PEST motif at the C terminus (CT) domain of Cx50 attenuates the stimulatory effect of Cx50 on lens fiber differentiation. Valine 362, a residue located within the PEST domain, is functionally involved. The structure of the Cx50 CT predicted by molecular modeling revealed four ␣-helices and Val 362 was found to be located in the middle of the 3rd helix. Replacement of Val 362 with amino acid residues that disrupt the ␣-helical structure predicted by molecular modeling, such as arginine, glutamate, or phenylalanine, attenuated the stimulatory effects of Cx50 on lens differentiation, whereas replacement with threonine, isoleucine, leucine, or proline, which maintain the structure preserved the function of Cx50. Circular dichroism (CD) studies supported the structural predictions and showed that the substitution with Glu, but not Thr or Pro, disrupted the ␣-helix, which appears to be the structural feature important for lens epithelial-fiber differentiation. Together, our results suggest that Val 362 is important for maintaining the helical structure and is crucial for the role of Cx50 in promoting lens epithelial-fiber differentiation.

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Lens cells: More than meets the eye

Cited by 32 publications

References 17 publications

miR-204 is required for lens and retinal development via Meis2 targeting

miR-204 is required for lens and retinal development via Meis2 targeting

Expression patterns of ADAMs in the developing chicken lens

Amino Acid Residue Val362 Plays a Critical Role in Maintaining the Structure of C Terminus of Connexin 50 and in Lens Epithelial-fiber Differentiation

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