Length-tension relationships in resting and activated vascular smooth muscle fibres

Peiper, U.; Schmidt, E.; Laven, Richard; Griebel, L.

doi:10.1007/bf00588170

Cited by 16 publications

(6 citation statements)

References 17 publications

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“…The length-tension curve obtained in the present study could be fit to an exponential relationship (Fig. 3), as has been reported in other vessels (25). From this relationship, a transmural pressure point of 3 cmH 2 O was calculated for normalization in each vessel, which was previously reported as optimal for the pump activity for the rat thoracic duct (9).…”

Section: Discussionsupporting

confidence: 70%

Stretch-induced calcium sensitization of rat lymphatic smooth muscle

Shirasawa

Benoit

2003

American Journal of Physiology-Heart and Circulatory Physiology

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Shirasawa, Yuichi, and Joseph N. Benoit. Stretchinduced calcium sensitization of rat lymphatic smooth muscle. Am J Physiol Heart Circ Physiol 285: H2573-H2577, 2003. First published August 28, 2003 10.1152/ajpheart.00002. 2003The relationships between smooth muscle calcium and isometric tension generation to spontaneous lymphatic pump activity and its modulation by stretch equivalent from 0 to ϳ6 cmH2O were investigated. Excised preparations of the rat thoracic duct were mounted on a wire myograph and loaded with the calcium-sensitive fluorochrome indo-1. Calcium-dependent fluorescence and isometric force were simultaneously recorded. The thoracic duct segments developed spontaneous rhythmic contractile activity. Each contraction was preceded by an increase in intracellular calcium. When the vessels were normalized and stabilized at a preload equal to 3 cmH2O, the peak generation in tension occurred 0.70 Ϯ 0.11 s after that of calcium. Incremental stretch enhanced the frequency of the phasic activity and amplitude of isometric force generation but not the basal calcium level or the amplitude of the calcium transient. These findings suggest that stretch enhances lymphatic pump activity by increasing the pacemaker activity and the calcium sensitivity of the contractile apparatus.

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Section: Discussionsupporting

confidence: 70%

Stretch-induced calcium sensitization of rat lymphatic smooth muscle

Shirasawa

Benoit

2003

American Journal of Physiology-Heart and Circulatory Physiology

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“…After mounting, vessels were equilibrated for half an hour in Krebs solution aerated with 95% O 2 and 5% CO 2 , while heated at 37°C. The passive wall tension internal circumference relationship was then determined to represent the internal in vivo circumference under a transmural pressure of 100 mmHg, to find a standard and physiologically representative setting for the diameter (Peiper et al 1973, Delaey et al 2002, Rinne et al 2012. After 45 min equilibration period, the arteries were contracted repeatedly with 62 mM KCl until maximal and reproducible contractions were obtained.…”

Section: Experiments Of Ex Vivo Vascular Reactivitymentioning

confidence: 99%

The effects of equal caloric high fat and western diet on metabolic syndrome, oxidative stress and vascular endothelial function in mice

et al. 2014

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“…After mounting, vessels were equilibrated, normalized, and contracted repeatedly with 62 mmol/L KCl until maximal and reproducible contractions were obtained. 21,22 To measure vasodilatation, arterial rings were pre-contracted with prostaglandin F 2a (aorta) or PE (mesenteric artery) and the contraction was adjusted to 50 -80% of the reference contraction to 62 mmol/L KCl. 23 Effects of NOS blockade on vascular responses were tested by applying L-NNA (100 mmol/L for 30 min).…”

Section: Vascular Reactivity Analysismentioning

confidence: 99%