Length and Charge of the N-terminus Regulate the Lifetime of the Signaling State of Photoactive Yellow Protein
Cheolhee Yang,
Youngmin Kim,
Seong Ok Kim
et al.
Abstract:Photoactive yellow protein (PYP) is one of the most extensively
studied photoreceptors. Nevertheless, the role of the N-terminus in
the photocycle and structural transitions is still elusive. Here,
we attached additional amino acids to the N-terminus of PYP and investigated
the effect of the length and charge of additional N-terminal residues
using circular dichroism, two-dimensional nuclear magnetic resonance
(2D-NMR), transient absorption (TA), and transient grating (TG) spectroscopic
techniques. TA experime… Show more
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