Lelliottia amnigena recovered from the lung of a harbour porpoise, and comparative analyses with Lelliottia spp.

Abstract

Strain M1325/93/1 (herein referred to by our laboratory identifier, GFKo1) of Lelliottia amnigena was isolated from the lung of a harbour porpoise in 1993. The genome sequence and antimicrobial resistance profile (genomic, phenotypic) of the strain were generated, with the genomic data compared with those from closely related bacteria. We demonstrate that the recently described chromosomally encoded AmpC β-lactamase bla LAQ is a core gene of L. amnigena , and suggest that new variants of this class of lactamase are encoded by other members of the genus Lelliottia . Although presence of bla LAQ is ubiquitous across the currently sequenced members of L. amnigena , we highlight that strain GFKo1 is sensitive to ampicillin and cephalosporins. These data suggest that bla LAQ may act as a useful genetic marker for identification of L. amnigena strains, but its presence may not correlate with expected phenotypic resistances. Further studies are required to determine the regulatory mechanisms of bla LAQ in L. amnigena .

Conserved patterns of sequence diversification provide insight into the evolution of two-component systems in Enterobacteriaceae

“…To analyse the evolution of TCS in Enterobacteriaceae , we first generated a set of reference genomes. We selected one strain from each of nine different genera within this family: Escherichia coli K-12 (strain MG1655), Salmonella enterica (serovar Typhimurium, strain LT2), Citrobacter rodentium (strain ICC168), Klebsiella variicola (strain 342; originally assigned to the K. pneumoniae species but later reassigned to the K. variicola species, which is a member of the K. pneumoniae complex), Cronobacter turicensis (strain z3032), Enterobacter cloacae (strain ATCC 13047), Phytobacter diazotrophicus (strain TA9730), Kosakonia sacchari (strain BO-1), and Huaxiibacter chinensis (strain ZB04; originally referred to as Lelliottia amnigena , but later assigned to H. chinensis ) [ 51 52 ]. The goal of this reference strain set was not to comprehensively capture the genetic diversity within this lineage, but rather to serve as a cross-section of Enterobacteriaceae TCS genetic diversity, such that individual strains represent genera that have diverged in ecology and lifestyle over millions of years.…”