Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil

Cavalcante, Giani Maria; Câmara, Celso A.; Silva, Eva Mônica Sarmento da; Santos, Mariana Silva dos; Leite, Anderson Brandão; Queiroz, Aline Cavalcanti de; Silva, Amanda Evelyn da; Araújo, Morgana Vital de; Alexandre-Moreira, Magna Suzana; Silva, Tânia Maria Sarmento

doi:10.3389/fphar.2021.702032

Cited by 8 publications

(7 citation statements)

References 37 publications

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“…Recently, compounds isolated from Brazilian propolis have demonstrated activity in vitro against Leishmania greater than that shown by the accepted treatment [ 8 ]. The bioavailability of propolis in humans is not well established, although there have been some limited studies of temperate propolis where the flavonoids, which are abundant in this type of propolis, appear to be well absorbed but are extensively metabolised [ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…Since propolis contains a multitude of chemical components in a complex mixture, its biological activity and pharmacological properties might similarly be broad [ 1 , 2 ]. Propolis has been observed to have promising activity against a number of protozoal species, particularly Trypanosoma and Leishmania species [ 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. Studies of the antiprotozoal activity of propolis have recently been reviewed [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes

Alenezi

Ebiloma

et al. 2022

Molecules

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Profiling a propolis sample from Papua New Guinea (PNG) using high-resolution mass spectrometry indicated that it contained several triterpenoids. Further fractionation by column chromatography and medium-pressure liquid chromatography (MPLC) followed by nuclear magnetic resonance spectroscopy (NMR) identified 12 triterpenoids. Five of these were obtained pure and the others as mixtures of two or three compounds. The compounds identified were: mangiferonic acid, ambonic acid, isomangiferolic acid, ambolic acid, 27-hydroxyisomangiferolic acid, cycloartenol, cycloeucalenol, 24-methylenecycloartenol, 20-hydroxybetulin, betulin, betulinic acid and madecassic acid. The fractions from the propolis and the purified compounds were tested in vitro against Crithidia fasciculata, Trypanosoma congolense, drug-resistant Trypanosoma congolense, Trypanosoma b. brucei and multidrug-resistant Trypanosoma b. brucei (B48). They were also assayed for their toxicity against U947 cells. The compounds and fractions displayed moderate to high activity against parasitic protozoa but only low cytotoxicity against the mammalian cells. The most active isolated compound, 20-hydroxybetulin, was found to be trypanostatic when different concentrations were tested against T. b. brucei growth.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes

Alenezi

Ebiloma

et al. 2022

Molecules

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“…Herbalists have recommended propolis according to its antibacterial, antiviral and anti-inflammatory activity, which can be used in the treatment of different types of infections as well as duodenal and gastric ulcers [ 1 , 8 ]. Many biological activities of propolis have been confirmed by modern studies, including anticancer [ 9 ], antioxidant [ 10 ], antileishmanial [ 11 ], wound healing [ 12 ], anti-inflammatory [ 13 ] and immunomodulatory [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Profile and Antimicrobial Potential of Propolis Samples from Kazakhstan

et al. 2023

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In the current paper, we present the results of Kazakh propolis investigations. Due to limited data about propolis from this country, research was focused mainly on phytochemical analysis and evaluation of propolis antimicrobial activity. uHPLC-DAD (ultra-high-pressure-liquid chromatography coupled with diode array detection, UV/VIS) and uHPLC-MS/MS (ultra-high-pressure-liquid chromatography coupled with tandem mass spectrometry) were used to phytochemical characteristics while antimicrobial activity was evaluated in the serial dilution method (MIC, minimal inhibitory concentration, and MBC/MFC, minimal bactericidal/fungicidal concentration measurements). In the study, Kazakh propolis exhibited a strong presence of markers characteristic of poplar-type propolis—flavonoid aglycones (pinocembrin, galangin, pinobanksin and pinobanskin-3-O-acetate) and hydroxycinnamic acid monoesters (mainly caffeic acid phenethyl ester and different isomers of caffeic acid prenyl ester). The second plant precursor of Kazakh propolis was aspen–poplar with 2-acetyl-1,3-di-p-coumaroyl glycerol as the main marker. Regarding antimicrobial activity, Kazakh propolis revealed stronger activity against reference Gram-positive strains (MIC from 31.3 to above 4000 mg/L) and yeasts (MIC from 62.5 to 1000 mg/L) than against reference Gram-negative strains (MIC ≥ 4000 mg/L). Moreover, Kazakh propolis showed good anti-Helicobacter pylori activity (MIC and MBC were from 31.3 to 62.5 mg/L). All propolis samples were also tested for H. pylori urease inhibitory activity (IC50, half-maximal inhibitory concentration, ranged from 440.73 to 11,177.24 µg/mL). In summary Kazakh propolis are potent antimicrobial agents and may be considered as a medicament in the future.

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“…Studies have demonstrated the leishmanicidal effect of different extracts of propolis both in vitro and in vivo ( Devequi-Nunes et al, 2018 ; Regueira-Neto et al, 2018 ; Cavalcante et al, 2021 ). Previously, our group described the leishmanicidal and immunomodulatory effects of three different extracts of green propolis in Leishmania braziliensis infection in vitro, as well as directly on the promastigote forms of this parasite ( Rebouças-Silva et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Leishmanicidal and immunomodulatory properties of Brazilian green propolis extract (EPP-AF®) and a gel formulation in a pre-clinical model

Rebouças-Silva

Amorim²,

Jesus-Santos³

et al. 2023

Front. Pharmacol.

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Leishmaniasis is a widespread group of neglected vector-borne tropical diseases that possess serious therapeutic limitations. Propolis has been extensively used in traditional medical applications due to its range of biological effects, including activity against infectious agents. Here we evaluated the leishmanicidal and immunomodulatory properties of Brazilian green propolis extract (EPP-AF®) and a gel formulation incorporating EPP-AF®, in both in vitro and in vivo models of Leishmania amazonensis infection. Propolis extract, obtained from a standardized blend following hydroalcoholic extraction, showed the characteristic fingerprint of Brazilian green propolis as confirmed by HPLC/DAD. A carbopol 940 gel formulation was obtained containing propolis glycolic extract at 3.6% w/w. The release profile, assessed using the Franz diffusion cell protocol, demonstrated a gradual and prolonged release of p-coumaric acid and artepillin C from the carbomer gel matrix. Quantification of p-coumaric acid and artepillin C in the gel formulation over time revealed that p-coumaric acid followed the Higuchi model, dependent on the disintegration of the pharmaceutical preparation, while artepillin C followed a zero-order profile with sustained release. In vitro analysis revealed the ability of EPP-AF® to reduce the infection index of infected macrophages (p < 0.05), while also modulating the production of inflammatory biomarkers. Decreases in nitric oxide and prostaglandin E2 levels were observed (p < 0.01), suggesting low iNOS and COX-2 activity. Furthermore, EPP-AF® treatment was found to induce heme oxygenase-1 antioxidant enzyme expression in both uninfected and L. amazonensis-infected cells, as well as inhibit IL-1β production in infected cells (p < 0.01). ERK-1/2 phosphorylation was positively correlated with TNF-α production (p < 0.05), yet no impact on parasite load was detected. In vivo analysis indicated the effectiveness of topical treatment with EPP-AF® gel alone (p < 0.05 and p < 0.01), or in combination with pentavalent antimony (p < 0.05 and p < 0.001), in the reduction of lesion size in the ears of L. amazonensis-infected BALB/c mice after seven or 3 weeks of treatment, respectively. Taken together, the present results reinforce the leishmanicidal and immunomodulatory effects of Brazilian green propolis, and demonstrate promising potential for the EPP-AF® propolis gel formulation as a candidate for adjuvant therapy in the treatment of Cutaneous Leishmaniasis.

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Leismanicidal Activity of Propolis Collected in the Semiarid Region of Brazil

Cited by 8 publications

References 37 publications

The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes

The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes

Phytochemical Profile and Antimicrobial Potential of Propolis Samples from Kazakhstan

Leishmanicidal and immunomodulatory properties of Brazilian green propolis extract (EPP-AF®) and a gel formulation in a pre-clinical model

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