Leishmanicidal effects of piperine, its derivatives, and analogues on Leishmania amazonensis

Ferreira, Christian; Soares, Deivid Costa; Junior, Cleber Bomfim Barreto; Nascimento, Michelle T. C.; Freire-de-Lima, Leonardo; Delorenzi, Jan Carlo; Lima, Marco Edílson Freire de; Atella, Geórgia C.; Folly, Evelize; Carvalho, Técia Maria Ulisses de; Saraiva, Elvira M.; Pinto-da-Silva, Lúcia Helena

doi:10.1016/j.phytochem.2011.08.006

Cited by 77 publications

(51 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The infected macrophages were treated with 100 M resveratrol for 24 h or left untreated, were washed and incubated with 0.01% SDS for 10 min, followed by the addition of 1 ml of Schneider's medium and 10% FCS, and were cultured at 26°C for 2 days. The relative intracellular load of viable L. amazonensis amastigotes was measured by a parasite rescue and transformation assay (26,27).…”

Section: Methodsmentioning

confidence: 99%

“…BALB/c peritoneal macrophages were treated with resveratrol and amphotericin B and incubated at 37°C in 5% CO 2 for 24 h. The macrophages were infected with L. amazonensis promastigotes at a 10:1 parasite-to-cell ratio for 1 h, and the rate of association was determined by randomly counting Ն200 cells in each of the duplicate coverslips (26).…”

Section: Methodsmentioning

confidence: 99%

“…After 6 h of incubation, the nitrite concentration was determined by the Griess method. The results were expressed as the M concentration of nitrite calculated in comparison with the sodium nitrite standard curve (26).…”

Section: Methodsmentioning

confidence: 99%

“…The promastigotes were treated with or without 100 M resveratrol and 0.1 M amphotericin B for 48 h. JC-1 (5 g/ml) staining solution (prepared according to the manufacturer's instructions) was added to the 10 6 cells for 20 min at 37°C. The ⌬⌿m was measured at 490 nm excitation and 530 nm emission (for JC-1 monomers) and 525 nm excitation and 590 nm emission wavelengths (for J-aggregates) in a SpectraMax Paradigm (Molecular Devices) using 96-well opaque culture plates (26).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Resveratrol Is Active against Leishmania amazonensis: In Vitro Effect of Its Association with Amphotericin B

Ferreira

Soares

Nascimento

et al. 2014

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Resveratrol is a polyphenol found in black grapes and red wine and has many biological activities. In this study, we evaluated the effect of resveratrol alone and in association with amphotericin B (AMB) against Leishmania amazonensis. Our results demonstrate that resveratrol possesses both antipromastigote and antiamastigote effects, with 50% inhibitory concentrations (IC 50 s) of 27 and 42 M, respectively. The association of resveratrol with AMB showed synergy for L. amazonensis amastigotes, as demonstrated by the mean sums of fractional inhibitory index concentration (mean ⌺FIC) of 0.483, although for promastigotes, this association was indifferent. Treatment with resveratrol increased the percentage of promastigotes in the sub-G 0 /G 1 phase of the cell cycle, reduced the mitochondrial potential, and showed an elevated choline peak and CH 2 -to-CH 3 ratio in the nuclear magnetic resonance (NMR) spectroscopy analysis; all these features indicate parasite death. Resveratrol also decreased the activity of the enzyme arginase in uninfected and infected macrophages with and without stimulation with interleukin-4 (IL-4), also implicating arginase inhibition in parasite death. The anti-Leishmania effect of resveratrol and its potential synergistic association with AMB indicate that these compounds should be subjected to further studies of drug association therapy in vivo.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Resveratrol Is Active against Leishmania amazonensis: In Vitro Effect of Its Association with Amphotericin B

Ferreira

Soares

Nascimento

et al. 2014

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…A total of 10,000 events were acquired in the region that corresponded to the parasites. The percentages of cells in each stage of the cell cycle were determined (22). Evaluation of lipid bodies.…”

Section: Methodsmentioning

confidence: 99%

Dibenzylideneacetones Are Potent Trypanocidal Compounds That Affect the Trypanosoma cruzi Redox System

Lazarin-Bidóia

Desoti

Martins

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Despite ongoing efforts, the available treatments for Chagas' disease are still unsatisfactory, especially in the chronic phase of the disease. Our previous study reported the strong trypanocidal activity of the dibenzylideneacetones A3K2A1 and A3K2A3 against Trypanosoma cruzi (Z. Ud Din, T. P. Fill, F. F. de Assis, D. Lazarin-Bidóia, V. Kaplum, F. P. Garcia, C. V. Nakamura, K. T. de Oliveira, and E. Rodrigues-Filho, Bioorg Med Chem 22:1121-1127, 2014, http://dx.doi.org/10.1016/j.bmc.2013.12.020). In the present study, we investigated the mechanisms of action of these compounds that are involved in parasite death. We showed that A3K2A1 and A3K2A3 induced oxidative stress in the three parasitic forms, especially trypomastigotes, reflected by an increase in oxidant species production and depletion of the endogenous antioxidant system. This oxidative imbalance culminated in damage in essential cell structures of T. cruzi, reflected by lipid peroxidation and DNA fragmentation. Consequently, A3K2A1 and A3K2A3 induced vital alterations in T. cruzi, leading to parasite death through the three pathways, apoptosis, autophagy, and necrosis.

show abstract

An Update on Natural Antileishmanial Treatment Options from Plants, Fungi and Algae

Koko

Nasr

Khan

et al. 2021

Chemistry & Biodiversity

View full text Add to dashboard Cite

Efficient drugs for the treatment of leishmaniasis, which is classified as a neglected tropical disease, are sought for. This review covers potential drug candidates from natural plant, fungus and algae sources, which were described over the last six years. The identification of these natural antileishmanials often based on the knowledge of traditional medicines. Crucial insights into the activities of these natural remedies against Leishmania parasites and against infections caused by these parasites in laboratory animals or patients are provided and compared with selected former active examples published more than six years ago. In addition, immuno-modulatory natural antileishmanials and recent developments on combination therapies including natural products and approved antileishmanials are discussed. The described natural products revealed promising data warranting further efforts on the discovery and development of new antileishmanials based on patterns from nature.

show abstract

Leishmanicidal effects of piperine, its derivatives, and analogues on Leishmania amazonensis

Cited by 77 publications

References 48 publications

Resveratrol Is Active against Leishmania amazonensis: In Vitro Effect of Its Association with Amphotericin B

Resveratrol Is Active against Leishmania amazonensis: In Vitro Effect of Its Association with Amphotericin B

Dibenzylideneacetones Are Potent Trypanocidal Compounds That Affect the Trypanosoma cruzi Redox System

An Update on Natural Antileishmanial Treatment Options from Plants, Fungi and Algae

Contact Info

Product

Resources

About