Leishmania ( V .) braziliensis infecting bats from Pantanal wetland, Brazil: First records for Platyrrhinus lineatus and Artibeus planirostris

Ferreira, Eduardo; Pereira, Agnes Antônio Sampaio; Silveira, Maurício; Margonari, Carina; Marcon, Gláucia Elisete Barbosa; França, Adriana de Oliveira; Castro, Ludiele Souza; Bordignon, Marcelo Oscar; Fischer, Erich Arnold; Tomás, Walfrido Moraes; Dorval, Maria Elizabeth Cavalheiros; Gontijo, Célia Maria Ferreira

doi:10.1016/j.actatropica.2017.05.012

Cited by 21 publications

(7 citation statements)

References 38 publications

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“…Examples include demonstration of canine infection by L. ( Viannia ) guyanensis in the Brazilian Amazon using PCR and sequencing of HSP70 genes, upon DNA extraction from blood samples [ 41 ]. The presence of Leishmania parasites in the skin of different species of bats was also evaluated using kDNA PCR and PCR-RFLP analysis of the HSP70 gene in specimens collected in the Pantanal wetlands in Brazil [ 42 ] and also using nested PCR targeting the small subunit ribosomal RNA (SSU rRNA) gene in specimens from central western Brazil [ 43 ]. Also, PCR targeting ITS1 genes demonstrated Leishmania presence in different species of rodents in VL-endemic areas in Ethiopia [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia

et al. 2022

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Background Leishmaniasis is endemic in Tunisia and presents with different clinical forms, caused by the species Leishmania infantum, Leishmania major, and Leishmania tropica. The life cycle of Leishmania is complex and involves several phlebotomine sand fly vectors and mammalian reservoir hosts. The aim of this work is the development and evaluation of a high-resolution melting PCR (PCR-HRM) tool to detect and identify Leishmania parasites in wild and domestic hosts, constituting confirmed (dogs and Meriones rodents) or potential (hedgehogs) reservoirs in Tunisia. Methods Using in vitro-cultured Leishmania isolates, PCR-HRM reactions were developed targeting the 7SL RNA and HSP70 genes. Animals were captured or sampled in El Kef Governorate, North West Tunisia. DNA was extracted from the liver, spleen, kidney, and heart from hedgehogs (Atelerix algirus) (n = 3) and rodents (Meriones shawi) (n = 7) and from whole blood of dogs (n = 12) that did not present any symptoms of canine leishmaniasis. In total, 52 DNA samples were processed by PCR-HRM using both pairs of primers. Results The results showed melting curves enabling discrimination of the three Leishmania species present in Tunisia, and were further confirmed by Sanger sequencing. Application of PCR-HRM assays on reservoir host samples showed that overall among the examined samples, 45 were positive, while seven were negative, with no Leishmania infection. Meriones shawi were found infected with L. major, while dogs were infected with L. infantum. However, co-infections with L. major/L. infantum species were detected in four Meriones specimens and in all tested hedgehogs. In addition, multiple infections with the three Leishmania species were found in one hedgehog specimen. Sequence analyses of PCR-HRM products corroborated the Leishmania species found in analyzed samples. Conclusions The results of PCR-HRM assays applied to field specimens further support the possibility of hedgehogs as reservoir hosts of Leishmania. In addition, we showed their usefulness in the diagnosis of canine leishmaniasis, specifically in asymptomatic dogs, which will ensure a better evaluation of infection extent, thus improving elaboration of control programs. This PCR-HRM method is a robust and reliable tool for molecular detection and identification of Leishmania and can be easily implemented in epidemiological surveys in endemic regions. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia

et al. 2022

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“…Recently, 6.4% (3/47) of Brazilian bats have been detected infected with L . brazilinensis , two Platyrrhinus lineatus and one Artibeus planirostris [54]. Leishmania spp.…”

Section: Discussionmentioning

confidence: 99%

“…By contrast, Shapiro et al . reported a 40% higher prevalence among bats from an endemic area of Brazil [54]. These results indicate that further studies are needed to assess the role of bats in maintaining the life cycle of leishmaniasis, especially in areas where these diseases are endemic.…”

Section: Discussionmentioning

confidence: 99%

Potential animal reservoirs (dogs and bats) of human visceral leishmaniasis due to Leishmania infantum in French Guiana

Medkour

Davoust

Dulieu³

et al. 2019

PLoS Negl Trop Dis

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In French Guiana, cutaneous leishmaniasis is highly endemic, whereas no autochthonous case of visceral leishmaniasis have been reported so far. However, due to its proximity to Brazil which is highly endemic for visceral leishmaniasis, and the high transboundary population flow, an epidemiological challenge could arise at any time. As an overseas department and region and the largest outermost region of the European Union, epidemiological surveillance of visceral leishmaniasis is of great importance. Our study aimed to investigate the presence of Leishmania spp. in domestic (dogs) and sylvatic (bats) animals from French Guiana. Over the 2008–2018 period, samples from 349 animals were collected. They included blood from 179 autochthonous dogs and 59 bats, spleen samples from 33 bats and, blood from 78 military working dogs (MWD) collected before their departure from continental France and at the end of their four-month stay in French Guiana. Samples were screened using real-time polymerase chain reaction (qPCR) assays targeting Leishmania DNA followed by sequencing of 18S rRNA, kDNA and ITS2 genes. L . infantum was detected in 2.3% (8/349) of animals with 1.7% (3/179) of autochthonous dogs, 5.1% (4/78) of MWD returning from French Guiana, whereas they were negative before their departure. One of them dates back to 2012. All these dogs were positive for serological tests. In addition, L . infantum DNA was detectable in one bat spleen sample, belonging to Carollia perspicillata species. We report here for the first time an infection with L . infantum in dogs and bat from French Guiana. Our results suggest the existence of potential reservoir and transmission cycle for visceral leishmaniasis, at least since 2012, which was unknown in this territory until now. Further studies are needed to determine how these animals were infected and which vectors are involved in the transmission in this area.

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“…Since then, four more studies found L. (Viannia) spp. DNA in more than eight species of bats including hematophagous, insectivorous and frugivorous individuals [32,[43][44][45].…”

Section: Wild Animals Infected With Zoonotic Leishmania (Viannia) Sppmentioning

confidence: 99%

A Systematic Review (1990–2021) of Wild Animals Infected with Zoonotic Leishmania

2021

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Leishmaniasis are neglected diseases caused by several species of Leishmania that affect humans and many domestic and wild animals with a worldwide distribution. The objectives of this review are to identify wild animals naturally infected with zoonotic Leishmania species as well as the organs infected, methods employed for detection and percentage of infection. A literature search starting from 1990 was performed following the PRISMA methodology and 161 reports were included. One hundred and eighty-nine species from ten orders (i.e., Carnivora, Chiroptera, Cingulata, Didelphimorphia, Diprotodontia, Lagomorpha, Eulipotyphla, Pilosa, Primates and Rodentia) were reported to be infected, and a few animals were classified only at the genus level. An exhaustive list of species; diagnostic techniques, including PCR targets; infected organs; number of animals explored and percentage of positives are presented. L. infantum infection was described in 98 wild species and L. (Viania) spp. in 52 wild animals, while L. mexicana, L. amazonensis, L. major and L. tropica were described in fewer than 32 animals each. During the last decade, intense research revealed new hosts within Chiroptera and Lagomorpha. Carnivores and rodents were the most relevant hosts for L. infantum and L. (Viannia) spp., with some species showing lesions, although in most of the studies clinical signs were not reported.

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Leishmania ( V .) braziliensis infecting bats from Pantanal wetland, Brazil: First records for Platyrrhinus lineatus and Artibeus planirostris

Cited by 21 publications

References 38 publications

High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia

High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia

Potential animal reservoirs (dogs and bats) of human visceral leishmaniasis due to Leishmania infantum in French Guiana

A Systematic Review (1990–2021) of Wild Animals Infected with Zoonotic Leishmania

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