Leishmania spp. Detection Using a Surface Plasmon Resonance Biosensor

Ferreira, Edlaine; Lima, Jefferson; Alves-Balvedi, Renata Pereira; Bonan, Paulo Rogério Ferreti; Medeiros, Eliton S.; Goulart, Luiz; Lima, Antonio Marcus Nogueira; Neff, H.; Oliveira, Leiva Casemiro; Castellano, Lúcio Roberto Cançado; Araújo, A. Pacheco; Moreira, Cleumar S.

doi:10.3390/proceedings1040536

Cited by 8 publications

(3 citation statements)

References 5 publications

(6 reference statements)

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“…A DNA-based biosensor using SPR that targeted the kDNA detected L. major and L. tropica [ 245 ]. Also using SPR biosensing techniques, Ferreira et al [ 245 ] developed an immunosensor based on circulating antibodies against L. infantum , which achieved antibody detection within 7 min [ 246 ]. Another DNA detection biosensor, which uses fluorescent probes based on the kDNA of L. infantum and nanostructured films as sensing platforms, provided sensitive results (1.1 nM of target DNA) even for complex sample types such a human blood [ 247 ].…”

Section: Methods For the Identification Of Species Of Leish...mentioning

confidence: 99%

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

et al. 2022

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Leishmania infections span a range of clinical syndromes and impact humans from many geographic foci, but primarily the world’s poorest regions. Transmitted by the bite of a female sand fly, Leishmania infections are increasing with human movement (due to international travel and war) as well as with shifts in vector habitat (due to climate change). Accurate diagnosis of the 20 or so species of Leishmania that infect humans can lead to the successful treatment of infections and, importantly, their prevention through modelling and intervention programs. A multitude of laboratory techniques for the detection of Leishmania have been developed over the past few decades, and although many have drawbacks, several of them show promise, particularly molecular methods like polymerase chain reaction. This review provides an overview of the methods available to diagnostic laboratories, from traditional techniques to the now-preferred molecular techniques, with an emphasis on polymerase chain reaction-based detection and typing methods. Graphical abstract

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Section: Methods For the Identification Of Species Of Leish...mentioning

confidence: 99%

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

et al. 2022

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“…The most common forms are visceral leishmaniasis, also known as kala-azar, and cutaneous leishmaniasis. Visceral leishmaniasis is the most severe variation of leishmaniasis and is caused by the species Leishmania donovani and Leishmania infantum (54)(55)(56)(57)(58).…”

Section: Leishmaniasismentioning

confidence: 99%

Low-Cost Microfluidic Systems for Detection of Neglected Tropical Diseases

Pinheiro

Guinati

Moreira

et al. 2023

Annual Rev. Anal. Chem.

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Neglected tropical diseases (NTDs) affect tropical and subtropical countries and are caused by viruses, bacteria, protozoa, and helminths. These kinds of diseases spread quickly due to the tropical climate and limited access to clean water, sanitation, and health care, which make exposed people more vulnerable. NTDs are reported to be difficult and inefficient to diagnose. As mentioned, most NTDs occur in countries that are socially vulnerable, and the lack of resources and access to modern laboratories and equipment intensify the difficulty of diagnosis and treatment, leading to an increase in the mortality rate. Portable and low-cost microfluidic systems have been widely applied for clinical diagnosis, offering a promising alternative that can meet the needs for fast, affordable, and reliable diagnostic tests in developing countries. This review provides a critical overview of microfluidic devices that have been reported in the literature for the detection of the most common NTDs over the past 5 years. Expected final online publication date for the Annual Review of Analytical Chemistry, Volume 16 is June 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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“…A SPR‐based biosensor on mono/polychromatic sources was proposed for highly specific and sensitive detection of Leishmania chagasi antigens. The rapid operation time with high sensitivity and good specificity was reported for this biosensor 13 . In another report, a gold nanoparticle‐based biosensing strategy was developed for sensitive detection of Leishmania DNA genome with both visual and spectrophotometric techniques.…”

Section: Introductionmentioning

confidence: 99%

Binding of Leishmania spp with gold nanoparticles supported polyethylene glycol and its application for the sensitive detection of infectious photogenes in human plasma samples: A novel biosensor

Mobed

Mehri

Hasanzadeh

et al. 2020

J of Molecular Recognition

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The management of pathogen detection using a rapid and cost-effective method presents a major challenge to the biological safety of the world. The field of pathogen detection is nascent and therefore, faces a dynamic set of challenges as the field evolves. Visceral leishmaniasis (VL), or kala-azar is the most severe form of leishmaniasis.Delay to the accurate diagnosis and treatment is likely to lead to fatality. The reliable, fast and sensitive detection is closely linked to safe and effective treatment of Leishmania spp. Despite several routine and old method for sensitive and specificity detection of Leishmania spp, there is highly demand for developing modern and powerfully system. In this study a novel ultra-sensitive DNA-based biosensor was prepared for detection of Leishmania spp. For the first time, the specific and thiolated sequences of the Leishmania spp genome (5 0 -SH-[CH 2 ] 6 ATCTCGTAAGCAGATCGCTGTGTCAC-3 0 ) were recognized by electrochemical methods. Also, selectivity of the proposed bioassay was examined by three sequences that were mismatched in 1, 2, and 3 nucleotides.The linear range (10 −6 to 10 −21 M) and limit of detection (LLOQ = 1 ZM) obtained are remarkable in this study. Also, simple and cost-effective construction of genosensors was another advantage of the proposal DNA-based assay. The experimental results promise a fast and simple method in detection of kala-azar patients with huge potential of the nanocomposite-based probe for development of ideal biosensors.

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Leishmania spp. Detection Using a Surface Plasmon Resonance Biosensor

Cited by 8 publications

References 5 publications

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

Low-Cost Microfluidic Systems for Detection of Neglected Tropical Diseases

Binding of Leishmania spp with gold nanoparticles supported polyethylene glycol and its application for the sensitive detection of infectious photogenes in human plasma samples: A novel biosensor

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