Leishmania species: Fatty acid composition of promastigotes

Vessal, Mahmood; Rezai, Hamid R.; Pakzad, Parviz

doi:10.1016/0014-4894(74)90085-x

Cited by 11 publications

(5 citation statements)

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“…In our analysis involving Leishmania major, we found that the principal fatty acids of the total lipids within the different classes were C14:0, C16:0, C20:2n-6, C20:3n-6, and C22:6n-3. Despite the variety of techniques and/or platforms used to identify FA composition, similar findings have been previously reported for L. tropica, L. mexicana, L. donovani, L. amazonensis, and L. tarentolae [18][19][20]29,30]. We noted the absence of C22:5 (n-6), detected in L. donovani and infantum [12], the absence of C18:3, found abundant in L. donovani PC [31] as well as C18:4 and C20:4 (n-3) not detected in L. donovani and L. infantum [12].…”

Section: Discussionsupporting

confidence: 83%

“…Previous studies from the 60s [18,19,29] have tried to determine the lipid profile of different Leishmania species using different techniques and platforms. In our analysis involving Leishmania major, we found that the principal fatty acids of the total lipids within the different classes were C14:0, C16:0, C20:2n-6, C20:3n-6, and C22:6n-3.…”

Section: Discussionmentioning

confidence: 99%

“…Their abundance and composition can vary depending on different factors such as pathogen species, availability, de novo synthesis capacities, lipid recycling, physiological environment, and response to treatments. Lipid profiles have been investigated in several species of Leishmania such as L. donovani, L. braziliensis, L. mexicana, L. tropica, L. amazonensis, L. tarentolae, L. enrietti, and L. infantum [12,[17][18][19][20]. Investigating lipids in L. major have mainly focused on identifying genes/proteins involved in one specific lipid pathway such as sphingolipid D4desaturase/C4-hydroxylases [21] or alkyl dihydroxyacetonephosphate synthase LmADS and dihydroxyacetonephosphate acyltransferase LmDAT were found essential for the synthesis of all ether glycerolipids [22,23].…”

Section: Introductionmentioning

confidence: 99%

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Fatty Acid Profiles of Leishmania major Derived from Human and Rodent Hosts in Endemic Cutaneous Leishmaniasis Areas of Tunisia and Algeria

et al. 2022

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Leishmaniasis is a protozoal vector-borne disease that affects both humans and animals. In the Mediterranean Basin, the primary reservoir hosts of Leishmania spp. are mainly rodents and canids. Lipidomic approaches have allowed scientists to establish Leishmania spp. lipid profiles for the identification of cell stage specific biomarkers, drug mechanisms of action, and host immune response. Using an in silico approach of global network interaction between genes involved in fatty acid (FA) synthesis followed by the GC-MS approach, we were able to characterize the fatty acid profiles of L. major derived from human and rodent hosts. Our results revealed that the lipid profile of L. major showed similarities and differences with those already reported for other Leishmania species. Phospholipids are the predominant lipid class. FA composition of rodent parasites was characterized by a lower abundance of the precursor C18:2(n-6). One of the rodent clones, which also expressed the lowest lipid abundance in PL and TAG, was the least sensitive clone to the miltefosine drug and has the lowest infection efficiency. Our findings suggest that the lipid composition variation may explain the response of the parasite toward treatment and their ability to infect their host.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fatty Acid Profiles of Leishmania major Derived from Human and Rodent Hosts in Endemic Cutaneous Leishmaniasis Areas of Tunisia and Algeria

et al. 2022

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“…The fatty acid composition for L. major was obtained from Vessal et al (1974). Chain lengths with less than 2% prevalence were not taken into account and the percentages of the remaining fatty acids were normalized.…”

Section: Methodsmentioning

confidence: 99%

Systems analysis of metabolism in the pathogenic trypanosomatid Leishmania major

Chavali

Whittemore

Eddy

et al. 2008

Molecular Systems Biology

138

142

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Systems analyses have facilitated the characterization of metabolic networks of several organisms. We have reconstructed the metabolic network of Leishmania major, a poorly characterized organism that causes cutaneous leishmaniasis in mammalian hosts. This network reconstruction accounts for 560 genes, 1112 reactions, 1101 metabolites and 8 unique subcellular localizations. Using a systemsbased approach, we hypothesized a comprehensive set of lethal single and double gene deletions, some of which were validated using published data with approximately 70% accuracy. Additionally, we generated hypothetical annotations to dozens of previously uncharacterized genes in the L. major genome and proposed a minimal medium for growth. We further demonstrated the utility of a network reconstruction with two proof-of-concept examples that yielded insight into robustness of the network in the presence of enzymatic inhibitors and delineation of promastigote/amastigote stage-specific metabolism. This reconstruction and the associated network analyses of L. major is the first of its kind for a protozoan. It can serve as a tool for clarifying discrepancies between data sources, generating hypotheses that can be experimentally validated and identifying ideal therapeutic targets.

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“…(h) Lipid content analysis (Vessal et al, 1974;Fish et al, 1981;Holz and Beach, 1982). (i) Radiorespirometry (Decker-Jackson et al, 1977;Decker-Jackson and Tang, 1982).…”

Section: Biochemical and Molecular Biological Structure And Functionmentioning

confidence: 99%

Identity and taxonomy within the genus Leishmania

Schnur

1986

Int. J. Trop. Insect Sci.

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Identification and taxonomy of the genus Leishmania is considered in brief, drawing on existing review articles. A fully-referenced synopsis of clinical, epidemiological, biological, immunological and biochemical criteria and methods used for typing leishmanial parasites is given and discussed. Classical and modern approaches are compared. Leishmanial phylogeny, the value of numerical taxonomy and the possibility of sexual recombination in leishmanial organisms are also considered. Resume-Identificationet la taxonomie dans le genre Leishmania sont rappeles brievement, pour attirer l'attention sur les revues et les articles existents. Les criteres et les methodes cliniques epidemiologiques, biologiques, immunologiques et biochimiques utilises pour typer ces parasites sont resumes dans un tableau synoptique et discute. Les approaches classiques et modernes sont comparees entre elles. La phylogenie des Leishmania, l'utilite de la taxonomie numerique et la possibility de recombinaison sexualle dans les Leishmania sont egalement discutees.

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Leishmania species: Fatty acid composition of promastigotes

Cited by 11 publications

References 9 publications

Fatty Acid Profiles of Leishmania major Derived from Human and Rodent Hosts in Endemic Cutaneous Leishmaniasis Areas of Tunisia and Algeria

Fatty Acid Profiles of Leishmania major Derived from Human and Rodent Hosts in Endemic Cutaneous Leishmaniasis Areas of Tunisia and Algeria

Systems analysis of metabolism in the pathogenic trypanosomatid Leishmania major

Identity and taxonomy within the genus Leishmania

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