Leishmania infantum transfected with toxic plasmid induces protection in mice infected with wild type L. infantum or L. amazonensis

Zorgi, Nahiara Esteves; Arruda, Leonardo Vicentini; Paladine, Izadora Baptista; Roque, Guilherme Augusto Sanches; Araújo, Thalita Fernanda; Brocchi, Marcelo; Barral, Manoel; Sanchiz, África; Requena, José Marı́a; Abánades, Daniel R.; Giorgio, Selma

doi:10.1016/j.molimm.2020.08.006

Cited by 5 publications

(3 citation statements)

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“…There is also evidence of the protective capacity of subunit vaccines based on antigens of parasite species that cause VL, such as vaccines formulated with ribosomal proteins from L. infantum (LRP) [16], some domains of the L. donovani nucleoside hydrolase (NH) [44], or a recombinant multiantigenic-composed DNA vaccine (HisAK70) generated by different DNA regions encoding several proteins from L. infantum [18]. Finally, it was reported recently that inoculation of L. infantum parasites programmed for their destruction in the host (because of the expression of genes encoding proteins toxic for the parasite) protects BALB/c mice against a L. amazonesis challenge [22].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, BALB/c mice were injected with L. infantum promastigotes bearing genes encoding for toxic proteins able to kill the amastigote forms in the vertebrate host. They presented a less severe disease when challenged with L. amanonensis, namely a reduction in skin lesions and parasite load compared to the animals of a control group [22]. Regarding the use attenuated lines generated by genetic modification, it has been shown that BALB/c mice vaccinated with a dihydrofolate-reductase thymidylate synthase L. major null mutant (Lmdhfr-ts -) were partially protected against a L. amazonensis infective challenge administered in the short term [23].…”

Section: Introductionmentioning

confidence: 99%

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Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice

et al. 2021

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Leishmania amazonensis parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB/c mice are highly susceptible to L. amazonensis challenge due to their inability to mount parasite-dependent IFN-γ-mediated responses. Here, we analyzed the capacity of a single administration of the LiΔHSP70-II genetically-modified attenuated L. infantum line in preventing cutaneous leishmaniasis in mice challenged with L. amazonensis virulent parasites. In previous studies, this live attenuated vaccine has demonstrated to induce long-protection against murine leishmaniasis due to Old World Leishmania species. Vaccinated mice showed a reduction in the disease evolution due to L. amazonensis challenge, namely reduction in cutaneous lesions and parasite burdens. In contrast to control animals, after the challenge, protected mice showed anti-Leishmania IgG2a circulating antibodies accompanied to the induction of Leishmania-driven specific IFN-γ systemic response. An analysis performed in the lymph node draining the site of infection revealed an increase of the parasite-specific IFN-ϒ production by CD4+ and CD8+ T cells and a decrease in the secretion of IL-10 against leishmanial antigens. Since the immunity caused by the inoculation of this live vaccine generates protection against different forms of murine leishmaniasis, we postulate LiΔHSP70-II as a candidate for the development of human vaccines.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice

et al. 2021

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“…In Leishmania , there have been several studies using mCherry to monitor the course of infection as this reporter is suitable for intravital imaging and enables deeper tissue penetration than GFP ( Calvo-Álvarez et al., 2012 ; Corman et al., 2019 ). mCherry was also used to evaluate the amastin expression in L. infantum in a stage-specific manner ( Zorgi et al., 2020 ). Besides, mCherry is one of the possible tag options which can be used in the T7 RNA polymerase-dependent CRISPR-Cas9 toolkit system ( Beneke et al., 2017 ), and the glmS ribozyme-based knock-down system of L. tarentolae ( Turra et al., 2021 ).…”

Section: Enzymatic Systemsmentioning

confidence: 99%