Leishmania infantum -specific IFN-γ production in stimulated blood from dogs with clinical leishmaniosis at diagnosis and during treatment

Martínez-Orellana, Pamela; Marí-Martorell, Daniel; Montserrat‐Sangrà, Sara; Ordeix, Laura; Baneth, Gad; Solano‐Gallego, Laia

doi:10.1016/j.vetpar.2017.10.018

Cited by 32 publications

(49 citation statements)

References 55 publications

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“…This result aligns well with a previous work, which reported marked Leishmania-specific IFN-γ production in healthy Ibizan hounds when compared to control and sick dogs [28]. In general, production of this pro-inflammatory cytokine is correlated with disease resistance or mild disease [12,19,20,52], while lack of expression is described in experimentally infected symptomatic dogs [12] and in dogs with a strong humoral response and severe disease [17,18]. Contrary to expectations, the proportion of positive LST reactions was not substantially different between groups, although a previous study described a higher frequency of positive results in Ibizan hounds [26].…”

Section: Discussionsupporting

confidence: 92%

“…A single non-Ibizan hound presented with clinical signs consistent with diagnosis of papular dermatitis, but later cytological examination of the papule suggested that it could be due to an eosinophilic arthropod bite reaction, as no amastigotes were detected and it did not display the typical "volcanic" appearance (Additional file 2: Figure S2). This dog was confirmed as infected by low positive 18 Ibizan hounds (definitive diagnosis) by means of visualization of amastigotes on skin samples (n = 9) or by molecular detection in stained smears (n = 9). As determined by these methods, the proportion of dogs with a definitive diagnosis of papular dermatitis due to Leishmania infection was statistically significant between breed groups (Fisher's exact test: P < 0.0001).…”

Section: Diagnosis Of Papular Dermatitismentioning

confidence: 91%

“…Another method consists of monitoring inflammatory cytokine IFN-γ expression in sera or peripheral blood stimulated with L. infantum soluble antigen (LSA). Lack of IFN-γ production has been reported in sick dogs [16], markedly so in dogs with severe disease [17,18], whereas greater levels of expression have been observed in dogs with subclinical infections or with mild to moderate disease [19,20]. Diagnosis of papular dermatitis also could be considered an indicator, as the relatively benign condition has been associated with a marked protective cellular immune response and a dampened humoral immune response [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Exploring the relationship between susceptibility to canine leishmaniosis and anti-Phlebotomus perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in Mallorca, Spain

et al. 2020

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Background: Canine leishmaniosis caused by Leishmania infantum is a neglected zoonosis transmitted by sand flies like Phlebotomus perniciosus. Clinical signs and disease susceptibility vary according to various factors, including host immune response and breed. In particular, Ibizan hounds appear more resistant. This immunocompetence could be attributed to a more frequent exposure to uninfected sand flies, eliciting a stronger anti-sand fly saliva antibody response. Methods: This study aimed to investigate the prevalence of anti-P. perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in the Leishmania-endemic area of Mallorca, Spain, and to correlate these antibody levels with clinical, immunological and parasitological parameters. Anti-sand fly saliva IgG was examined in 47 Ibizan hounds and 45 dogs of other breeds using three methods: P. perniciosus whole salivary gland homogenate (SGH) ELISA; recombinant protein rSP03B ELISA; and rSP03B rapid tests (RT). Additionally, diagnostic performance was evaluated between methods. Results: Results indicate significantly higher anti-SGH antibodies (P = 0.0061) and a trend for more positive SGH ELISA and RT results in Ibizan hounds compared to other breeds. General linear model analysis also found breed to be a significant factor in SGH ELISA units and a marginally significant factor in RT result. Although infection rates were similar between groups, Ibizan hounds included significantly more IFN-γ producers (P = 0.0122) and papular dermatitis cases (P < 0.0001). Older age and L. infantum seropositivity were also considered significant factors in sand fly saliva antibody levels according to at least one test. Fair agreement was found between all three tests, with the highest value between SGH and rSP03B RT. Conclusions: To our knowledge, this is the first study elaborating the relationship between anti-P. perniciosus saliva antibodies and extensive clinical data in dogs in an endemic area. Our results suggest that Ibizan hounds experience a higher frequency of exposure to sand flies and have a stronger cellular immune response to L. infantum infection than other breed dogs. Additional sampling is needed to confirm results, but anti-P. perniciosus saliva antibodies appear to negatively correlate with susceptibility to L. infantum infection and could possibly contribute to the resistance observed in Ibizan hounds.

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Section: Discussionsupporting

confidence: 92%

Section: Diagnosis Of Papular Dermatitismentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Exploring the relationship between susceptibility to canine leishmaniosis and anti-Phlebotomus perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in Mallorca, Spain

et al. 2020

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“…IFN-γ expression/production by sera or by peripheral blood mononuclear cell (PBMC) lymphocytes non-stimulated or stimulated with soluble Leishmania antigen (SLA) from naturally and experimentally infected dogs was correlated with resistance to disease, asymptomatic status or mild disease (Santos-Gomes et al, 2002 ; Manna et al, 2006 ; Carrillo et al, 2007 ; Aslan et al, 2016 ; Abbehusen et al, 2017 ; Montserrrat-Sangrà et al, 2018 )$. The lack of its production was observed both in symptomatic infected dogs (Carrillo et al, 2007 ) and in sick dogs with strong humoral response, high parasitaemia, and severe clinical disease (Solano-Gallego et al, 2016 ; Martínez-Orellana et al, 2017 ). On the other hand, high levels of IFN-γ production and expression was detected in naturally and experimentally infected dogs classified as symptomatic, indicating that this cytokine does not seem to be a good marker of resistance as it was not enough to prevent disease (Travi et al, 2009 ; Cortese et al, 2013 ).…”

Section: Immunological Biomarkers Of Susceptibility and Resistance Tomentioning

confidence: 99%

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

Maia

Campino

2018

Front. Cell. Infect. Microbiol.

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Canine leishmaniosis (CanL) is a vector-borne disease caused by the protozoan Leishmania (Leishmania) infantum species [syn. L. (L.) infantum chagasi species in the Americas] which is transmitted by the bite of a female phlebotomine sand fly. This parasitosis is endemic and affect millions of dogs in Asia, the Americas and the Mediterranean basin. Domestic dogs are the main hosts and the main reservoir hosts for human zoonotic leishmaniosis. The outcome of infection is a consequence of intricate interactions between the protozoan and the immunological and genetic background of the host. Clinical manifestations can range from subclinical infection to very severe disease. Early detection of infected dogs, their close surveillance and treatment are essential to control the dissemination of the parasite among other dogs, being also a pivotal element for the control of human zoonotic leishmaniosis. Hence, the identification of biomarkers for the confirmation of Leishmania infection, disease and determination of an appropriate treatment would represent an important tool to assist clinicians in diagnosis, monitoring and in giving a realistic prognosis to subclinical infected and sick dogs. Here, we review the recent advances in the identification of Leishmania infantum biomarkers, focusing on those related to parasite exposure, susceptibility to infection and disease development. Markers related to the pathogenesis of the disease and to monitoring the evolution of leishmaniosis and treatment outcome are also summarized. Data emphasizes the complexity of parasite-host interactions and that a single biomarker cannot be used alone for CanL diagnosis or prognosis. Nevertheless, results are encouraging and future research to explore the potential clinical application of biomarkers is warranted.

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“…Solano-Gallego et al (2016) demonstrated that infected dogs presenting high levels of IFN-γ had lower parasite loads when compared to infected dogs that did not produce this cytokine. Dogs lacking this cytokine have more severe clinical symptoms, with higher parasitemia (Martínez-Orellana et al, 2017). Similarly, Th17 cells induce L. infantum control growth (Nascimento et al, 2015;Rodriguez-Cortes et al, 2017).…”

Section: General Aspects Of the Immunological Profile And Biomarkers mentioning

confidence: 99%

An Overview of Immunotherapeutic Approaches Against Canine Visceral Leishmaniasis: What Has Been Tested on Dogs and a New Perspective on Improving Treatment Efficacy

Gonçalves

Leite

Resende

et al. 2019

Front. Cell. Infect. Microbiol.

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Leishmania infantum -specific IFN-γ production in stimulated blood from dogs with clinical leishmaniosis at diagnosis and during treatment

Cited by 32 publications

References 55 publications

Exploring the relationship between susceptibility to canine leishmaniosis and anti-Phlebotomus perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in Mallorca, Spain

Exploring the relationship between susceptibility to canine leishmaniosis and anti-Phlebotomus perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in Mallorca, Spain

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

An Overview of Immunotherapeutic Approaches Against Canine Visceral Leishmaniasis: What Has Been Tested on Dogs and a New Perspective on Improving Treatment Efficacy

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