Leishmania donovani: Assessment of leishmanicidal effects of herbal extracts obtained from plants in the visceral leishmaniasis endemic area of Bihar, India

Singh, Shubhankar Kumar; Bimal, Sanjiva; Narayan, Shyam; Jee, Chandrawati; Bimal, Devla; Das, Pradeep; Bimal, Raageeva

doi:10.1016/j.exppara.2010.10.014

Cited by 55 publications

(27 citation statements)

References 28 publications

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“…MACS™ (Miltenyi Biotech, USA) sorted monocytes were isolated using a human monocyte isolation negative selection kit (Miltenyi Biotech, USA) according to the manufacturer's instructions. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation over Histopaque-1077 (Sigma, USA) from heparinized human peripheral blood [36]. The cells were washed and resuspended at 1 Â 10 5 /ml in RPMI-1640 supplemented with 25 mM HEPES, 2 mM L-glutamine, 0.2% NaHCO 3 , 10% FBS (Hyclone) and 50 lg/ml gentamicin.…”

Section: Involvement Of Nkt With L Donovanimentioning

confidence: 99%

Leishmania donovani skews the CD56+ Natural Killer T cell response during human visceral leishmaniasis

et al. 2015

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Section: Involvement Of Nkt With L Donovanimentioning

confidence: 99%

Leishmania donovani skews the CD56+ Natural Killer T cell response during human visceral leishmaniasis

et al. 2015

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“…According to Singh et al [42], the extracts obtained from the leaves of Murraya koenigii (Linn. ), also known as Curry leaf have shown their antileishmanial activity against L. donovani promastigotes, however, we have generated the data for the curry leaf oil against L. donovani axenic as well as intracellular amastigotes.…”

Section: Discussionmentioning

confidence: 99%

Search for New Antileishmanial Chemotherapeutics

Kar

Ghosh

Kumari

et al. 2018

Int J Pharm Pharm Sci

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Objective: The objective of this work was to screen a number of compounds for their antileishmanial efficacy and cytotoxicity profiling.Methods: Curry leaf oil, cypress oil and spikenard oil were identified by gas chromatography-mass spectrometry (GC/MS) analysis. Betulinic acid, spikenard oil, cypress oil and curry leaf oil were evaluated for their in vitro antileishmanial activity against Leishmania donovani AG83 wild-type, sodium stibogluconate resistant (SSG-resistant), paromomycin (PMM-resistant) and GE1 field type strains on axenic and cellular amastigote model and compared the results with standard drugs used to treat leishmaniasis. Results:Betulinic acid showed strong antileishmanial activity against wild-type (SI= 192.8), SSG-resistant (SI= 19.3) and GE1 strains (SI= 100), whereas cypress oil has produced highest antileishmanial activity against PMM-resistant strains (SI= 15.09) among all the tested drugs. The data obtained also revealed that cypress oil had the maximum CC50 Conclusion: All tested drugs had antileishmanial property but among them, betulinic acid possess strong antileishmanial activity in case of both wild-type and drug-resistant leishmaniasis.value of 452.9 μl among all standard and tested drugs.

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“…The use of lectins as biotechnological approaches are justified by a large number of scientific studies showing biological relevant activities related to these proteins (Kitada et al, 2010, Kimble et al, 2010Singh et al, 2010;Cao et al, 2010). Among these biological activities, it is noted that lectins exhibit antibacterial activity (Alencar et al, 2005;Holanda et al, 2005, Wong et al, 2010, and lectins have ability to interfere with process of microbial biofilms formation (Teixeira et al, 2006Islam et al, 2009;Cavalcante et al, 2011).…”

Section: Lectins: Concept and Characteristicsmentioning

confidence: 99%

Plant lectins as alternative tools against bacterial biofilms

Cavalcante¹,

Firmino²,

Tajra³

et al. 2014

Afr. J. Microbiol. Res.

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Plant lectins has the ability to interfere in crucial biofilm formation aspects, playing a significant role in evaluation of patients at high and low risk of disease development. However, there is limited studies on the physiological role of lectins on bacteria living in biofilms like caries process. Thus, we aimed to provide a view of lectins biotechnological potential against bacterial biofilms development. Biofilm is a structured bacterial consortium that provides essential compounds for its survivor. This microorganism organization occurs naturally, since this arrangement increases its survival possibility. Bacterial biofilms are related to human health problems and are responsible for many infectious diseases, such as oral diseases, associated with inert surfaces, including medical devices for internal and external use. Thus, lectins are a large group of heterogeneous proteins that exhibit antibacterial activity, as well as ability to interfere with microbial biofilms formation process. The lectins ability to form complexes with microbial glycoconjugates has stimulated its application as probes to the whole cell, as well as its mutants and numerous cellular constituents and metabolites. Thus, the impact of bacterial resistance provided by biofilm formation on human health encourages researches aiming to understand biofilm mechanisms as well as strategies to eradicate or minimize these communities damages.

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Leishmania donovani: Assessment of leishmanicidal effects of herbal extracts obtained from plants in the visceral leishmaniasis endemic area of Bihar, India

Cited by 55 publications

References 28 publications

Leishmania donovani skews the CD56+ Natural Killer T cell response during human visceral leishmaniasis

Leishmania donovani skews the CD56+ Natural Killer T cell response during human visceral leishmaniasis

Search for New Antileishmanial Chemotherapeutics

Plant lectins as alternative tools against bacterial biofilms

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