Leishmania braziliensis ssp. in the nasal mucosa of guinea pigs inoculated in the tarsi

Abstract

Two lots of 20 young male guinea pigs were inoculated subcutaneously in the tarsi with 10(4) amastigotes of Leishmania braziliensis braziliensis or L. b. guyanensis to study the susceptibility of this Neotropical hystricomorph rodent the autochthonous parasites. Almost 50% of the animals showed lesions in the inoculation site and had parasitizations that were infective to hamsters, as shown by inoculating homogenates of the dermal lesion, of the spleen, of the liver, and of the nasal mucosa into hamsters at 20, 40, 60, and 120 days after inoculation of the guinea pig. Smears of the above organs showed the presence of amastigotes. Parasites inoculated into the tarsi were detected early in the skin, spleen, and liver of the guinea pig host. Blood cultures made by cardiopuncture on sacrifice of the guinea pigs were uniformly negative. The nasal mucosa of nearly all animals positive in the skin or viscera was invaded early by the parasites, although with greater frequency between 60 and 120 days post-inoculation. The use of this model for the study of mucocutaneous parasitism by L. braziliensis is discussed, together with the phenomena of parasitism at a distance from the inoculation site, the temperature of the body regions affected, and the possible genetic influence on susceptibility of the guinea pig to L. braziliensis.

Use of Cavia porcellus (guinea pigs) as an experimental model for Leishmania (Viannia) braziliensis

“…No cellular infiltration was observed in the histopathological exam. (15). Despite the results shown by this author, the search for mucous metastasis was negative using various laboratory methods, including PCR.…”

“…New drugs have been discovered to act against leishmaniasis, and laboratory animals are fundamental to study the efficacy of these drugs. showed promising results (11,15).…”