Leishmania braziliensis panamensis: Increased infectivity resulting from heat shock

Smejkal, Ruthann M.; Wolff, Ruth; Olenick, John G.

doi:10.1016/0014-4894(88)90101-4

Cited by 54 publications

(23 citation statements)

References 24 publications

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“…Some Leishmania sp. also transform to an amastigote-like form and increase their virulence characteristics after heat exposure (19,(49)(50)(51)(52). In our hands incubation at 370C constitutes a "heat shock" for L. d. chagasi, defined as an increase in the production of hsp7O.…”

Section: Discussionmentioning

confidence: 71%

Hydrogen peroxide-mediated toxicity for Leishmania donovani chagasi promastigotes. Role of hydroxyl radical and protection by heat shock.

1991

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Leishmania must survive despite exposure to the toxic oxidant hydrogen peroxide (H202) during phagocytosis by macrophages. We investigated the mechanism of H202 toxicity for L. donovani chagasi promastigotes, and factors responsible for their relative H202 resistance. There was a dose-dependent toxic effect of H202 for promastigotes isolated during logarithmic phase of growth. In contrast, stationary phase promastigotes were less susceptible to H202 toxicity, and more infectious for BALB/c mice. By spin trapping we found that hydroxyl radical ( * OH) was generated after exposure of promastigotes to H202, and the amount of -OH was greater with log-phase than with stationary-phase promastigotes.* OH was generated after the addition of H202 to the cytosol but not the membranes of fractionated promastigotes, and the magnitude of * OH was greater in log than in stationary promastigote cytosol. Deferoxamine inhibition suggested that intracellular promastigote iron catalyzes * OH formation via the Fenton reaction. Furthermore, exposure of log-phase promastigotes to heat shock induced a relative H202-resistant state, which was not associated with a decrease in -OH formation but which required ongoing transcription. Thus, growth to stationary phase and heat shock both induce a state of relative H202 resistance, but these are probably due to different resistance mechanisms. (J. Clin.

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Section: Discussionmentioning

confidence: 71%

Hydrogen peroxide-mediated toxicity for Leishmania donovani chagasi promastigotes. Role of hydroxyl radical and protection by heat shock.

1991

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“…HSPs in parasite infections appear, on the one hand, to play important roles in adaptation of microorganisms. For example, they may play roles in differentiation of parasites and in development of infectivity (16,17). On the other hand, HSPs function as prominent antigenic proteins that can activate the host immune systems (10).…”

Section: Discussionmentioning

confidence: 99%

Induction of heat shock protein closely correlates with protection against Toxoplasma gondii infection.

Nagasawa¹,

Oka²,

Maeda³

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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Heat shock proteins (HSPs) are evolutionarily highly conserved polypeptides that appear to be produced by many cells to preserve cellular functions under a variety of conditions of stress, including infections. We report that a 65-kDa HSP is present in mouse peritoneal cells that have been infected with a low-virulence (Beverley) strain of Toxoplasma gondii, as determined by electroblot assay using a monoclonal antibody specific for microbial HSP65. This HSP is, however, not expressed when infection occurs with the high-virulence

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“…4-6, 16 , 17, 30-33 Promastigotes of L. braziliensis were exposed to heat shock by changing the temperature of their culture from 26°C to 34°C. 6 Completely defined serum-free medium was used for conservation of infectivity in vitro . 16 Attempts were made to increase infectivity of parasites by co-culture with J774 macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…4 Therefore, investigations have been performed to enhance the infectivity and virulence of Leishmania parasites in vitro culture. [4][5][6] In one study, it was shown that infectivity and virulence changed dependent on the parasite species and the medium that was used. 5 In another study, infectivity of Leishmania brasiliensis promastigotes was found to be increased when they were exposed to heat shock.…”

Section: Introductionmentioning

confidence: 99%

“…5 In another study, infectivity of Leishmania brasiliensis promastigotes was found to be increased when they were exposed to heat shock. 6 Several studies have shown the stimulatory effect of human urine on Leishmania promastigotes. [7][8][9][10][11] When supplemented with human urine, Schneider's Drosophila culture media was found to increase the proliferation of different Leishmania parasite species.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Human Urine on Cell Cycle and Infectivity of Leismania Species Promastigotes In Vitro

Allahverdiyev

Bağırova²,

Elcicek³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. In vitro cultivation of Leishmania parasites plays an important role in diagnosis and treatment of leishmaniasis and in vaccine and drug development studies. Conversely, long-term cultivation of Leishmania parasites usually results in decreased infectivity potential. Some studies reported a stimulatory effect of human urine in Leishmania promastigotes. However, there is no information about the effects of urine within culture on the infectivity of Leishmania parasites. Analysis of the effect of urine have showed that proliferation indexes were significantly increased in culture medium supplemented with human urine ( L. tropica = 38.17 ± 5.12, L. donovani = 34.74 ± 5.6, L. major = 34.22 ± 4.66, and L. infantum 35.88 ± 6.40) than in controls. Infection indexes were 13 ± 1.7 for L. tropica , 55 ± 2.2 for L. infantum , 41 ± 3.14 for L. donovani , and 49 ± 3.26 for L. major . Our results showed that human urine increased the infectivity and proliferation of Leishmania parasites.* Address correspondence to Adil M. Allahverdiyev,

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Leishmania braziliensis panamensis: Increased infectivity resulting from heat shock

Cited by 54 publications

References 24 publications

Hydrogen peroxide-mediated toxicity for Leishmania donovani chagasi promastigotes. Role of hydroxyl radical and protection by heat shock.

Hydrogen peroxide-mediated toxicity for Leishmania donovani chagasi promastigotes. Role of hydroxyl radical and protection by heat shock.

Induction of heat shock protein closely correlates with protection against Toxoplasma gondii infection.

Effect of Human Urine on Cell Cycle and Infectivity of Leismania Species Promastigotes In Vitro

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