Leishmania amazonensis: Increase in ecto-ATPase activity and parasite burden of vinblastine-resistant protozoa

Giarola, Naira Lígia Lima; Silveira, Timotheo Souza; Inacio, Job D. F.; Paes-Vieira, Lisvane; Almeida-Amaral, Elmo Eduardo; Meyer‐Fernandes, José Roberto

doi:10.1016/j.exppara.2014.08.013

Cited by 13 publications

(13 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, inoculation with an isolate that presents lower ecto‐ATPase activity induced a less severe tissue injury, without necrosis or ulceration (Souza et al, ). The results obtained by Lima‐Giarola et al () suggest a relationship between the increase in Mg 2+ ‐dependent ecto‐ATPase activity from vinblastine‐resistant L. amazonensis promastigotes and greater infectivity and severity of the disease.…”

Section: Leishmania Amazonensis Ntpdasesmentioning

confidence: 94%

“…Furthermore, the parasite loads in the ears infected with vinblastine‐resistant promastigotes were also significantly higher than those found in the ears of mice inoculated with control cells. This result suggests that the increased severity of the disease in mice was caused by inoculation with the resistant parasite (Lima‐Giarola et al, ). Similarly, inoculation with an isolate that presents lower ecto‐ATPase activity induced a less severe tissue injury, without necrosis or ulceration (Souza et al, ).…”

Section: Leishmania Amazonensis Ntpdasesmentioning

confidence: 97%

“…Leishmania amazonensis promastigotes that were resistant to vinblastine, an antimicrotubule agent that blocks cell division, showed increased ecto‐ATPase activity and caused more severe disease (Lima‐Giarola et al, ). Mg 2+ ‐dependent ATPase activity was significantly higher in the resistant parasites than in the controls.…”

Section: Leishmania Amazonensis Ntpdasesmentioning

confidence: 99%

“…However, the presence of vinblastine in the reaction medium had no effects on ecto‐ATPase activity, excluding the possibility that ecto‐ATPase was modulated by residual vinblastine. Additionally, ecto‐ATPase protein expression was twofold higher in the resistant promastigotes than in the control cells (Lima‐Giarola et al, ).…”

Section: Leishmania Amazonensis Ntpdasesmentioning

confidence: 99%

See 3 more Smart Citations

NTPDase activities: possible roles on Leishmania spp infectivity and virulence

Paes-Vieira

Gomes-Vieira

Meyer‐Fernandes

2018

Cell Biology International

Self Cite

View full text Add to dashboard Cite

Nucleoside triphosphate diphosphohydrolases (NTPDases) are enzymes that belong to the GDA1/CD39 protein superfamily. These enzymes catalyze the hydrolysis of ATP and ADP to the monophosphate form (AMP). Biochemical characterization of the nucleotidases/NTPDases from various types of cells, including those from plants, animals, and pathogenic organisms, has revealed the existence of several isoforms with different specificities with respect to divalent cations (magnesium, calcium, manganese, and zinc) and substrates. In mammals, the NTPDases play important roles in the regulation of thrombosis and inflammation. In parasites of the genus Leishmania, the causative agents of leishmaniasis, two NTPDase isoforms, termed NTPDase-1 and NTPDase-2 have been described. Independently of their cellular localization, whether cell-surface localized, secreted or targeted to other organelles, in some Leishmania species these NTPDases could be involved in parasite growth, infectivity, and virulence. Experimental evidence has suggested that the hydrolysis of ATP and ADP by parasite ecto-nucleotidases can down-modulate the host immune response. In this context, the present work provides an overview of recent works that show strong evidence not only of the involvement of the nucleotidases/NTPDases in Leishmania spp infectivity and virulence but also of the molecular mechanisms that lead to the success of the parasitic infection.

show abstract

Section: Leishmania Amazonensis Ntpdasesmentioning

confidence: 94%

Section: Leishmania Amazonensis Ntpdasesmentioning

confidence: 97%

Section: Leishmania Amazonensis Ntpdasesmentioning

confidence: 99%

Section: Leishmania Amazonensis Ntpdasesmentioning

confidence: 99%

See 2 more Smart Citations

NTPDase activities: possible roles on Leishmania spp infectivity and virulence

Paes-Vieira

Gomes-Vieira

Meyer‐Fernandes

2018

Cell Biology International

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another group of researchers demonstrated that L. amazonesis resistant to vinblastine (a cell division inhibitor) showed an increase in the Mg 2+ -dependent ecto-ATPase activity and an increase in the severity of disease in infected mice [79] .…”

Section: Biochemical and Biological Roles Of Ecto-nucleotidases And Tmentioning

confidence: 99%

The Leishmania-Macrophage Interactions: Role of E-NTPDases and Purinergic Signaling

et al. 2016

View full text Add to dashboard Cite

Leishmaniasis comprises a group of diseases caused by protozoan parasites of the genus Leishmania. The transmission occurs by the bite of phlebotomine female sand flies of the genera Phlebotomus and Lutzomyia. In the mammalian host, these parasites infect and proliferate mainly into the macrophages, avoiding the harmful effects of the innate inflammatory response. It has been reported in several works that purinergic signaling, including purine receptors P1 and P2, are dynamically modulated during both the development and activation of cells from the immune system to achieve homeostasis or combat infections as well as harmful damage. Many pathogens are able to subvert the host immune response in order to promote the success or maintenance of infection. This subversion can be related to the influence of pathogens on purinergic signaling leading to decreased levels of the pro-inflammatory molecule ATP and increased levels of the immunosuppressive molecule adenosine. This scenario can be produced as the final product of the joint activity of E-NTPDases and 5'-ectonucleotidases. Thus, this review will discuss the cellular and molecular events occurring during the interaction of Leishmania and macrophages focusing on the purinergic signaling pathways and their relationships with ENTPDases from pathogenic species of Leishmania.

show abstract

Dependence of Leishmania parasite on host derived ATP: an overview of extracellular nucleotide metabolism in parasite

Arora

Kumar

2018

J Parasit Dis

View full text Add to dashboard Cite

The widespread role of ATP in humans has been characterized as a substrate for metabolism as well as other key processes occurring in the human system. Hence, the ATP pool may also be accessible to the invaders, including Leishmania donovani (a protozoan parasite), considering the fact that ATP is not scarce at the cellular location where the pathogen resides. The protozoan parasite survives in the human host by utilizing purines from its extracellular environment, due to its inability to synthesize purines de novo. The purines are accessible in the form of nucleoside triphosphates (NTPs), for example, adenosine (ADO) in the form of ATP molecules. These NTPs are processed by the ecto-nucleotidases in the transmembrane region of the parasite, which are then transported inside via nucleoside/ nucleobase transporters belonging to the ENT family of & Ambak Kumar Rai

show abstract

Leishmania amazonensis: Increase in ecto-ATPase activity and parasite burden of vinblastine-resistant protozoa

Cited by 13 publications

References 65 publications

NTPDase activities: possible roles on Leishmania spp infectivity and virulence

NTPDase activities: possible roles on Leishmania spp infectivity and virulence

The Leishmania-Macrophage Interactions: Role of E-NTPDases and Purinergic Signaling

Dependence of Leishmania parasite on host derived ATP: an overview of extracellular nucleotide metabolism in parasite

Contact Info

Product

Resources

About