Legumin and vicilin, storage proteins of legume seeds

Derbyshire, Eric; Wright, David J.; Boulter, D.

doi:10.1016/s0031-9422(00)89046-9

Cited by 919 publications

(510 citation statements)

References 174 publications

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“…In this study, the amount of it used in the agglutination assay was very high, indicating that bocaiúva globulin and glutelin fractions have very low lectin contents, in contrast with various plant seeds from which lectin has been isolated [7,20,29,33].…”

Section: -Discussionmentioning

confidence: 79%

“…Isolation of seed proteins has revealed that many plants (including Leguminosae) contain globulins as the main storage protein (50-75% of total seed protein) [5,7,23,31]. Studies regarding the degradation of legume proteins, particularly globulins, have demonstrated that cooking and fermentation separates this protein fraction from antinutritional factors, such as proteinase inhibitors and lectins, which is a feature that makes globulin suitable for studies concerning the nutritional value of proteins [5,7].…”

Section: -Discussionmentioning

confidence: 99%

“…The total protein fraction of seeds is a complex mixture of globulins (40-60%), albumins (8-20%), prolamins and glutelins, of which the first two constitute the main components. The proportions, however, differ among species, varieties and/or cultivars [7]. The literature reports that seed protein characterization and evaluation are central aspects in both basic and applied studies in nutrition and biotechnology areas [20,33].…”

Section: -Introductionmentioning

confidence: 99%

“…Storage proteins, such as lectins, ureases and proteolytic enzyme inhibitors correspond to 5% or more of the total protein present in seeds, reserve organs and other plant parts [7].…”

Section: -Introductionmentioning

confidence: 99%

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Chemical and nutritional evaluation of kernels of bocaiuva, Acrocomia aculeata (Jacq.) Lodd.

Hiane¹,

Baldasso²,

Marangoni³

et al. 2006

Ciênc. Tecnol. Aliment.

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SUMMARYProtein characterization and results of proximate composition and mineral analyses of fruit kernels of bocaiuva, Acrocomia aculeata (Jacq.) Lodd., are reported. The kernels presented high contents of oil (51.7%), protein (17.6%) and fiber (15.8%). The seeds´ soluble proteins were isolated according to their solubility. The main separated proteins were globulins (53.5%) and glutelins (40.0%). Moreover, the presence of low molecular mass proteases in these two fractions was shown by the SDS-PAGE method. The assays of proteaseinhibitory and hemagglutinating activities showed that bocaiuva´s protein fractions were not resistant to trypsin or chymotrypsin activities and that both had low lectin content. The globulin in vitro digestibility assay resembled a casein standard. Neither globulin nor glutelin enzymatic hydrolyses increased significantly (p < 0.05) after heat treatment. Threonine and lysine are the most limiting amino acids, respectively from two major protein fractions of the bocaiuva kernel, globulin (47.1% amino acid score) and glutelin (49.5% amino acid score), in terms of the theoretical profiles for children in the age range of 2 to 5 years recommended by the FAO/ WHO. Bocaiuva kernels are found to be rich in calcium, phosphorus and manganese compared to some fruit nuts such as cashew and coconut. Keywords: kernel, Acrocomia aculeata, globulin, glutelin, chemical analysis, digestibility. RESUMOAVALIAÇÃO QUÍMICA E NUTRICIONAL DE AMÊNDOAS DA BOCAIÚVA, Acrocomia aculeata (JACQ.) LODD. Caracterização de proteínas e análises da composição centesimal e teores de minerais foram realizados nas amêndoas da bocaiúva, Acrocomia aculeata (Jacq.) Lodd. As amêndoas apresentaram alto teor de lipídio (51,7%), proteína (17,6%) e fibra (15,8%). Proteínas solúveis das sementes foram fracionadas de acordo com a sua solubilidade. As principais proteínas separadas foram as globulinas (53,5%) e glutelinas (40,0%), e a presença de proteases de baixo peso molecular nessas duas frações foi revelada por eletroforese em gel de poliacrilamida (SDS-PAGE). Ensaios da atividade inibitória de proteases e da hemaglutinação mostraram que as frações protéicas da bocaiúva não foram resistentes à ação da tripsina e quimotripsina e apresentaram baixo teor de lectina. A digestibilidade in vitro da globulina foi semelhante à da caseína padrão. Hidrólises enzimáticas da globulina e glutelina não aumentaram significativamente (p < 0,05), com o aquecimento. Treonina e lisina são os aminoácidos mais limitantes, respectivamente, das duas principais frações de proteínas da amêndoa da bocaiúva, a globulina (escore de aminoácido de 47,1%) e glutelina (escore de aminoácido de 49,5%), relativamente ao padrão teórico para crianças de 2 a 5 anos de idade, recomendado pela FAO/WHO. Amêndoas de bocaiúva mostraram ser ricas em cálcio, fósforo e manganês, em comparação com algumas amêndoas de frutos como caju e coco.

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Section: -Discussionmentioning

confidence: 79%

Section: -Discussionmentioning

confidence: 99%

Section: -Introductionmentioning

confidence: 99%

“…Storage proteins, such as lectins, ureases and proteolytic enzyme inhibitors correspond to 5% or more of the total protein present in seeds, reserve organs and other plant parts [7].…”

Section: -Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chemical and nutritional evaluation of kernels of bocaiuva, Acrocomia aculeata (Jacq.) Lodd.

Hiane¹,

Baldasso²,

Marangoni³

et al. 2006

Ciênc. Tecnol. Aliment.

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“…When globulins from the scutellum were separated by isoelectric precipitation into crude 7S and 11 S fractions both preparations behaved as the EA in the double diffusion test (unpublished data). It is in fact most likely that we are dealing with the characteristic group of labile cereal globulins very similar to the well-known reserve globulins of legumes (for review see Derbyshire et al, 1976). A recent report by Horikoshi and Morita (1975) provides additional support for this suggestion.…”

Section: Discussionmentioning

confidence: 87%

Embryonal antigens in maize caryopses: The temporal order of antigen accumulation during embryogenesis

Хавкин

Misharin

Ivanov

et al. 1977

Planta

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Abstract. The sequential appearance of a specific group of embryonal antigens (EA), presumably globulins, was demonstrated in developing maize (Zea mays L.) caryopses using a double immunodiffusion test with absorption of common antigens. Cross immunoelectrophoresis was employed to follow the differential pattern of EA accumulation in the growing scutellum and embryonic axis. The transient nature of two predominant EA seems to indicate their role as specific protein reserves of embryonal tissues. Another presumably organ-specific EA was maintained in callus obtained from a 28-day-old culture of scutellum isolated from the mature non-germinated caryopsis.

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Physicochemical characterisation of mung bean (Phaseolus aureus) protein isolates

Rahma

Dudek²,

Mothes³

et al. 2000

J. Sci. Food Agric.

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Legumin and vicilin, storage proteins of legume seeds

Cited by 919 publications

References 174 publications

Chemical and nutritional evaluation of kernels of bocaiuva, Acrocomia aculeata (Jacq.) Lodd.

Chemical and nutritional evaluation of kernels of bocaiuva, Acrocomia aculeata (Jacq.) Lodd.

Embryonal antigens in maize caryopses: The temporal order of antigen accumulation during embryogenesis

Physicochemical characterisation of mung bean (Phaseolus aureus) protein isolates

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