Legume vicilins (7S storage globulins) inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells

Gomes, Valdirene Moreira; Okorokov, Lev A.; Rose, Tatiana Lundgren; Fernandes, Kátia Valevski Sales; Xavier‐Filho, J.

doi:10.1016/s0304-4165(97)00100-1

Cited by 43 publications

(31 citation statements)

References 24 publications

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“…Control experiments (Tris-HCl buffer was added instead of CaTI) were performed to evaluate the influence of CaTI on H ? extrusion by yeast cells (Gomes et al 1998). The concentration of H ?…”

Section: Nitric Oxide Induction Determination Assaymentioning

confidence: 99%

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Ribeiro

Silva

Cunha

et al. 2011

Antonie van Leeuwenhoek

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A 6,000 Da peptide, named CaTI, was isolated from Capsicum annuum L. seeds and showed potent inhibitory activity against trypsin and chymotrypsin. The aim of this study was to determine the effect of CaTI on Saccharomyces cerevisiae, Candida albicans, Candida tropicalis and Kluyveromyces marxiannus cells. We observed that CaTI inhibited the growth of S. cerevisiae, K. marxiannus as well as C. albicans and induced cellular agglomeration and the release of cytoplasmic content. No effect on growth was observed in C. tropicalis but morphological changes were noted. In the spot assay, different degrees of sensitivity were shown among the strains and concentrations tested. Scanning electron microscopy showed that S. cerevisiae, K. marxiannus and C. albicans, in the presence of CaTI, exhibited morphological alterations, such as the formation of pseudohyphae, cellular aggregates and elongated forms. We also show that CaTI induces the generation of nitric oxide and interferes in a dose-dependent manner with glucose-stimulated acidification of the medium mediated by H(+)-ATPase of S. cerevisiae cells.

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Section: Nitric Oxide Induction Determination Assaymentioning

confidence: 99%

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Ribeiro

Silva

Cunha

et al. 2011

Antonie van Leeuwenhoek

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“…The significance of this reassignment is that it further supports a functional link both to the other bacterial enzymes concerned with sugar metabolism (e.g., PMIs and epimerases) and to the higher-plant cupins, particularly the sucrose-binding proteins (detailed below). In this context, there is an additional circumstantial link between chitobiose and cupins, in that vicilins from cowpea (Vigna unguiculata) are known to bind chitin (248), and it has been suggested that the vicilin-induced inhibition of yeast cell growth is due to binding of the protein to the chitin component of the cell walls (96,97).…”

Section: Arac-type Transcription Factorsmentioning

confidence: 99%

Microbial Relatives of the Seed Storage Proteins of Higher Plants: Conservation of Structure and Diversification of Function during Evolution of the Cupin Superfamily

Dunwell

Khuri

Gane

2000

Microbiol Mol Biol Rev

308

296

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SUMMARY This review summarizes the recent discovery of the cupin superfamily (from the Latin term “cupa,” a small barrel) of functionally diverse proteins that initially were limited to several higher plant proteins such as seed storage proteins, germin (an oxalate oxidase), germin-like proteins, and auxin-binding protein. Knowledge of the three-dimensional structure of two vicilins, seed proteins with a characteristic β-barrel core, led to the identification of a small number of conserved residues and thence to the discovery of several microbial proteins which share these key amino acids. In particular, there is a highly conserved pattern of two histidine-containing motifs with a varied intermotif spacing. This cupin signature is found as a central component of many microbial proteins including certain types of phosphomannose isomerase, polyketide synthase, epimerase, and dioxygenase. In addition, the signature has been identified within the N-terminal effector domain in a subgroup of bacterial AraC transcription factors. As well as these single-domain cupins, this survey has identified other classes of two-domain bicupins including bacterial gentisate 1,2-dioxygenases and 1-hydroxy-2-naphthoate dioxygenases, fungal oxalate decarboxylases, and legume sucrose-binding proteins. Cupin evolution is discussed from the perspective of the structure-function relationships, using data from the genomes of several prokaryotes, especially Bacillus subtilis. Many of these functions involve aspects of sugar metabolism and cell wall synthesis and are concerned with responses to abiotic stress such as heat, desiccation, or starvation. Particular emphasis is also given to the oxalate-degrading enzymes from microbes, their biological significance, and their value in a range of medical and other applications.

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“…These proteins have different described mechanisms of action. For example, vicilins present affinity to chitin, binding to it and interfering with fungal growth by an unknown mechanism (Gomes et al 1997(Gomes et al , 1998. Chitinases and b-1,3-glucanases are hydrolases that break the b-1,3 linked glucans and b-1,4 linked N-acetyl-D-glucosamine, respectively, which are the two major structural components of the fungal cell wall, thus inhibiting their development (Carlile et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Defense Proteins from Vigna Unguiculata Seed Exudates: Characterization and Inhibitory Activity Against Fusarium Oxysporum

et al. 2006

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Plants exude a variety of substances through their roots, germinating seeds and aerial parts. Some of these released compounds seem to have an inhibitory effect against pathogens. The aim of this work was to investigate and identify antifungal proteins present in exudates from imbibed cowpea seeds (Vigna unguiculata (L.) Walp). The obtained exudation was analyzed in regard to specific protein activities by enzymatic or immunological assays for plant defense proteins, from 4 h to 48 h of seed imbibition. Our results show that cowpea seeds exudates present several defense related proteins characterized as b-1,3-glucanases, cystatins, vicilins and lipid transfer proteins (LTPs), as well as a storage vacuole membrane a-TIP protein, since the very first hours of imbibition. These exudates also have an ''in vitro'' inhibitory effect on the growth of the fungus Fusarium oxysporum f. sp. phaseoli. Our results suggest that seed exudates should promote seed protection from soil pathogens.

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Legume vicilins (7S storage globulins) inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells

Cited by 43 publications

References 24 publications

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Microbial Relatives of the Seed Storage Proteins of Higher Plants: Conservation of Structure and Diversification of Function during Evolution of the Cupin Superfamily

Defense Proteins from Vigna Unguiculata Seed Exudates: Characterization and Inhibitory Activity Against Fusarium Oxysporum

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