Legume–Rhizobium Symbioses as a Tool for Bioremediation of Heavy Metal Polluted Soils

Pajuelo, Eloísa; Rodríguez‐Llorente, Ignacio D.; Lafuente, Alejandro; Caviedes, Miguel A.

doi:10.1007/978-94-007-1914-9_4

Cited by 72 publications

(43 citation statements)

References 124 publications

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“…Neither soil properties nor oil concentration responded to the PGPB inoculation (data not shown) in our field, in contrast to other studies where PGPB enhanced the rhizoremediation of polluted soils (Pajuelo et al, 2011;Vershinina et al, 2012;Bhattacharyya and Jha, 2012). However, the oil tolerance ability of PGPB strains, the local field factors and the hydrocarbon composition were different from these studies.…”

Section: Pgpb Effectcontrasting

confidence: 85%

Perennial crop growth in oil-contaminated soil in a boreal climate

Yan

Penttinen

Simojoki

et al. 2015

Science of The Total Environment

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Soil contamination by petroleum hydrocarbons is a global problem. Phytoremediation by plants and their associated microorganisms is a cost-effective strategy to degrade soil contaminants. In boreal regions the cool climate limits the efficiency of phytoremediation. The planting of oil-tolerant perennial crops, especially legumes, in oil-contaminated soil holds promise for great economic benefits for bioenergy and bio-fertilizer production while accelerating the oil degradation process. We established a multi-year field experiment to study the ecological and agronomic feasibility of phytoremediation by a legume (fodder galega) and a grass (smooth brome) in a boreal climate. In 40 months, soil oil content decreased by 73%-92%, depending on the crop type. The oil degradation followed first-order kinetics with the reduction rates decreasing as follows: bare fallow > galega-brome grass mixture > brome grass > galega. Surprisingly, the presence of oil enhanced crop dry matter and nitrogen yield, particularly in the fourth year. The unfertilized galega-brome grass mixture out-yielded the N-fertilized pure grass swards over years by an average of 33%. Thus, a perennial legume-grass mixture is both ecologically and agronomically sustainable as a cropping system to alleviate soil contamination in the boreal zone, with considerable potential for bioenergy and bio-fertilizer production.

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Section: Pgpb Effectcontrasting

confidence: 85%

Perennial crop growth in oil-contaminated soil in a boreal climate

Yan

Penttinen

Simojoki

et al. 2015

Science of The Total Environment

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“…A plantderived peribacteroid membrane surrounds bacteroids, the symbiotic form of the bacteria, thus controlling nutrient exchange between both symbionts (22). Although the Rhizobium-legume symbiosis has been proposed as a tool for bioremediation of heavy metal-polluted soils (23,24), the information on determinants involved in metal resistance in Rhizobiaceae is scarce, restricted to a few reports on the levels of metal tolerance by members of this relevant group of endosymbiotic bacteria (25,26). However, this bacterial group might be a relevant reservoir of genetic determinants mediating survival under high-metal conditions, as deduced from the large number of metal resistance genes identified in the genome of a Mesorhizobium amorphae isolate obtained from a Zn/Pb mine tailing (27).…”

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confidence: 99%

Functional and Expression Analysis of the Metal-Inducible dmeRF System from Rhizobium leguminosarum bv. viciae

Rubio-Sanz

Prieto

Imperial

et al. 2013

Appl Environ Microbiol

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A gene encoding a homolog to the cation diffusion facilitator protein DmeF from Cupriavidus metallidurans has been identified in the genome of Rhizobium leguminosarum UPM791. The R. leguminosarum dmeF gene is located downstream of an open reading frame (designated dmeR) encoding a protein homologous to the nickel-and cobalt-responsive transcriptional regulator RcnR from Escherichia coli. Analysis of gene expression showed that the R. leguminosarum dmeRF genes are organized as a transcriptional unit whose expression is strongly induced by nickel and cobalt ions, likely by alleviating the repressor activity of DmeR on dmeRF transcription. An R. leguminosarum dmeRF mutant strain displayed increased sensitivity to Co(II) and Ni(II), whereas no alterations of its resistance to Cd(II), Cu(II), or Zn(II) were observed. A decrease of symbiotic performance was observed when pea plants inoculated with an R. leguminosarum dmeRF deletion mutant strain were grown in the presence of high concentrations of nickel and cobalt. The same mutant induced significantly lower activity levels of NiFe hydrogenase in microaerobic cultures. These results indicate that the R. leguminosarum DmeRF system is a metal-responsive efflux mechanism acting as a key element for metal homeostasis in R. leguminosarum under free-living and symbiotic conditions. The presence of similar dmeRF gene clusters in other Rhizobiaceae suggests that the dmeRF system is a conserved mechanism for metal tolerance in legume endosymbiotic bacteria.

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“…Rhizosphere bacteria have to adapt in order to survive in a metal-contaminated environment. PGPB secrete metabolites, such as extracellular polysaccharide, organic acid, siderophores, lipids, and proteins, which could be used for chelation, complexation, precipitation, and adsorption of heavy metals present in the rhizophere (32). Moreover, microbes could also resist, adsorb, accumulate, oxidize, and reduce metal ions themselves, thereby helping to improve phytoremediation (26).…”

Section: Isolation and Identification Of Agrobacterium Tumefaciens CCmentioning

confidence: 99%

Genome Sequence and Mutational Analysis of Plant-Growth-Promoting Bacterium Agrobacterium tumefaciens CCNWGS0286 Isolated from a Zinc-Lead Mine Tailing

Hao

Xie

Johnstone

et al. 2012

Appl Environ Microbiol

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The plant-growth-promoting bacterium Agrobacterium tumefaciens CCNWGS0286, isolated from the nodules of Robinia pseudoacacia growing in zinc-lead mine tailings, both displayed high metal resistance and enhanced the growth of Robinia plants in a metal-contaminated environment. Our goal was to determine whether bacterial metal resistance or the capacity to produce phytohormones had a larger impact on the growth of host plants under zinc stress. Eight zinc-sensitive mutants and one zinc-sensitive mutant with reduced indole-3-acetic acid (IAA) production were obtained by transposon mutagenesis. Analysis of the genome sequence and of transcription via reverse transcriptase PCR (RT-PCR) combined with transposon gene disruptions revealed that ZntA-4200 and the transcriptional regulator ZntR1 played important roles in the zinc homeostasis of A. tumefaciens CCNWGS0286. In addition, interruption of a putative oligoketide cyclase/lipid transport protein reduced IAA synthesis and also showed reduced zinc and cadmium resistance but had no influence on copper resistance. In greenhouse studies, R. pseudoacacia inoculated with A. tumefaciens CCNWGS0286 displayed a significant increase in biomass production over that without inoculation, even in a zinc-contaminated environment. Interestingly, the differences in plant biomass improvement among A. tumefaciens CCNWGS0286, A. tumefaciens C58, and zinc-sensitive mutants 12-2 (zntA::Tn5) and 15-6 (low IAA production) revealed that phytohormones, rather than genes encoding zinc resistance determinants, were the dominant factor in enhancing plant growth in contaminated soil. P lant-growth-promoting bacteria (PGPB) play a key role in host plant adaptation to metal-contaminated environments through triggering physiological changes in plant cell metabolism so that growing plants can tolerate high concentrations of transition or heavy metals (7,26). PGPB have been isolated from various plants in heavy-metal-contaminated environments (3, 47-49). They were able to enhance plant growth, make plants more tolerant to heavy and transition metals, and thus accelerate phytoremediation by mechanisms including nitrogen fixation, nitrogen and phosphorus metabolism, and the production of siderophores, organic acids, 1-aminocyclopropane-1-carboxylate deaminase (ACC), and phytohormones such as indole-3-acetic acid (IAA), cytokinins, acetoin, and 2,3-butanediol (21, 41, 54). It was reported previously that the existence of high concentrations of metals selected for more metal resistant PGPB to resist these adverse environmental conditions (20,26). However, it is often not known which trait of the PGPB, e.g., tolerance to heavy and transition metals, phytohormones, or a combination of the two, plays the dominant role in the survival of a given plant in a metal-contaminated environment.Agrobacterium tumefaciens is a soilborne alphaproteobacterium belonging to the family Rhizobiaceae whose ability to induce crown gall tumors on dicotyledonous plants makes it of great concern worldwide. Recently, many nonpath...

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Legume–Rhizobium Symbioses as a Tool for Bioremediation of Heavy Metal Polluted Soils

Cited by 72 publications

References 124 publications

Perennial crop growth in oil-contaminated soil in a boreal climate

Perennial crop growth in oil-contaminated soil in a boreal climate

Functional and Expression Analysis of the Metal-Inducible dmeRF System from Rhizobium leguminosarum bv. viciae

Genome Sequence and Mutational Analysis of Plant-Growth-Promoting Bacterium Agrobacterium tumefaciens CCNWGS0286 Isolated from a Zinc-Lead Mine Tailing

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