Legume Nitrogen Fixation and Soil Abiotic Stress: From Physiology to Genomics and Beyond

Valentine, Alex J.; Benedito, Vagner A.; Kang, Yun Hwan

doi:10.1002/9781444328608.ch9

Cited by 52 publications

(38 citation statements)

References 212 publications

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“…Therefore, the greater decrease in SNF capacity observed in MmSWRI9-induced nodules under low Pi conditions ( Fig. 1 and Dataset S1) cannot be related to the accumulation of the identified amino acids, which has been reported in other studies (5,38). The accumulation of specific amino acids in plants subjected to low Pi conditions, however, might be implicated in down-regulating SNF capacity in MmSWRI9-induced nodules relative to McCP-31-induced nodules.…”

Section: Discussionmentioning

confidence: 49%

“…Among the stressors, low Pi availability is a major restriction of SNF capacity, because this process depends on a series of energy-demanding metabolic steps. Reductions in the concentration of ATP and energy charge in Pi-deficient nodules result in significant declines in nitrogenase activity, SNF capacity, and ultimately, the growth and productivity of legume crops (5). Thus, an improvement in Pi levels in the nodules of plants grown in soils with low Pi availability can contribute to a more efficient atmospheric N 2 fixing capacity and therefore, greater productivity of legumes (6).…”

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confidence: 99%

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Adaptation of the symbiotic Mesorhizobium –chickpea relationship to phosphate deficiency relies on reprogramming of whole-plant metabolism

Esfahani

Kusano

Nguyen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Low inorganic phosphate (Pi) availability is a major constraint for efficient nitrogen fixation in legumes, including chickpea. To elucidate the mechanisms involved in nodule acclimation to low Pi availability, two Mesorhizobium-chickpea associations exhibiting differential symbiotic performances, Mesorhizobium ciceri CP-31 (McCP-31)-chickpea and Mesorhizobium mediterranum SWRI9 (MmSWRI9)-chickpea, were comprehensively studied under both control and low Pi conditions. MmSWRI9-chickpea showed a lower symbiotic efficiency under low Pi availability than McCP-31-chickpea as evidenced by reduced growth parameters and down-regulation of nifD and nifK. These differences can be attributed to decline in Pi level in MmSWRI9-induced nodules under low Pi stress, which coincided with up-regulation of several key Pi starvation-responsive genes, and accumulation of asparagine in nodules and the levels of identified amino acids in Pi-deficient leaves of MmSWRI9-inoculated plants exceeding the shoot nitrogen requirement during Pi starvation, indicative of nitrogen feedback inhibition. Conversely, Pi levels increased in nodules of Pi-stressed McCP-31-inoculated plants, because these plants evolved various metabolic and biochemical strategies to maintain nodular Pi homeostasis under Pi deficiency. These adaptations involve the activation of alternative pathways of carbon metabolism, enhanced production and exudation of organic acids from roots into the rhizosphere, and the ability to protect nodule metabolism against Pi deficiency-induced oxidative stress. Collectively, the adaptation of symbiotic efficiency under Pi deficiency resulted from highly coordinated processes with an extensive reprogramming of whole-plant metabolism. The findings of this study will enable us to design effective breeding and genetic engineering strategies to enhance symbiotic efficiency in legume crops.phosphate deficiency | nitrogen fixation | metabolomics | carbon and nitrogen metabolism | phosphate homeostasis P hosphorus plays a critical role in numerous plant metabolic processes and contributes to the biosynthesis of cellular macromolecules, such as ATP, nucleic acids, phospholipids, and phosphorylated sugars (1). Thus, phosphorus has been established as one of the most important elements required for normal plant growth and development (1). Unfortunately, limited availability of inorganic phosphate (Pi), which is the only absorbable form of phosphorus for plants, in soils is nearly universal, because Pi readily forms various insoluble compounds with metals, such as calcium and iron in alkaline and acidic soils, respectively (1, 2). Pi deficiency can be overcome by the application of Pi fertilizers; however, the excessive use of chemical fertilizers can have serious environmental consequences, including the contamination of soil and water resources (1). Additionally, the global demand for and use of Pi fertilizers are projected to increase significantly with the explosive growth of the global population.Thus, it has been predicted that global Pi res...

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Section: Discussionmentioning

confidence: 49%

mentioning

confidence: 99%

Adaptation of the symbiotic Mesorhizobium –chickpea relationship to phosphate deficiency relies on reprogramming of whole-plant metabolism

Esfahani

Kusano

Nguyen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…Maximization of the BFN process in leguminous crops can be compromised by several limiting properties to the establishment of symbiosis and nitrogen fixation efficiency. These include biotic factors such as competition with native soil bacteria, as well as abiotic factors such as temperature, humidity, and pH (Valentine et al 2010). The selection of efficient strains capable of performing BNF with agriculturally important plants has been the subject of extensive research, and in it rests the success of inoculant application in these crops (Thies et al 2001;Pinto et al 2007;Ribeiro et al 2009;Olivares et al 2013).…”

Section: Applicationmentioning

confidence: 99%

The Family Rhizobiaceae

Alves¹,

Souza²,

Varani³

et al. 2014

The Prokaryotes

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“…Plants thus depend heavily on P for plant growth and development, especially legume plants since P is required for biological nitrogen fixation (BNF) (Schulze et al 1999) and has been reported to affect the energy costs of BNF (Valentine et al 2010), as well as nodule formation and function (Israel 1987). Soil P is however, limited and its availability is contingent on various factors such as diffusion rates in the soil and solubilisation of P containing compounds (Vance et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Legume nodules from nutrient-poor soils exhibit high plasticity of cellular phosphorus recycling and conservation during variable phosphorus supply

Vardien

Steenkamp

Valentine

2016

Journal of Plant Physiology

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Nitrogen fixing legumes rely on phosphorus for nodule formation, nodule function and the energy costs of fixation. Phosphorus is however very limited in soils, especially in ancient sandstone-derived soils such as those in the Cape Floristic Region of South Africa. Plants growing in such areas have evolved the ability to tolerate phosphorus stress by eliciting an array of physiological and biochemical responses. In this study we investigated the effects of phosphorus limitation on N 2 fixation and phosphorus recycling in the nodules of Virgilia divaricata (Adamson), a legume native to the Cape Floristic Region. In particular, we focused on nutrient acquisition efficiencies, phosphorus fractions and the exudation and accumulation of phosphatases. Our finding indicate that during low phosphorus supply, V. divaricata internally recycles phosphorus and has a lower uptake rate of phosphorus, as well as lower levels adenylates but greater levels of phosphohydrolase exudation suggesting it engages in recycling internal nodule phosphorus pools and making use of alternate bypass routes in order to conserve phosphorus.

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Legume Nitrogen Fixation and Soil Abiotic Stress: From Physiology to Genomics and Beyond

Cited by 52 publications

References 212 publications

Adaptation of the symbiotic Mesorhizobium –chickpea relationship to phosphate deficiency relies on reprogramming of whole-plant metabolism

Adaptation of the symbiotic Mesorhizobium –chickpea relationship to phosphate deficiency relies on reprogramming of whole-plant metabolism

The Family Rhizobiaceae

Legume nodules from nutrient-poor soils exhibit high plasticity of cellular phosphorus recycling and conservation during variable phosphorus supply

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