Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells

Koriyama, Toyoyasu; Yamakuchi, Munekazu; Takenouchi, Kazunori; Oyama, Yoko; Takenaka, Hiroyoshi; Nagakura, Takumi; Masamoto, Izumi; Hashiguchi, Teruto

doi:10.1016/j.bbrc.2018.11.093

Cited by 5 publications

(3 citation statements)

References 40 publications

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“…Было показано, что после инфицирования клеточной культуры моноцитов L. pneumophila в клетках возрастала экспрессия генов SLIT2 и SLIT3, в интронах которых кодируется miR-218. В свою очередь, эта микроРНК ингибировала экспрессию гена RICTOR, усиливая продукцию провоспалительных цитокинов IL-6 и TNFα [43].…”

Section: микрорнкunclassified

Non-coding RNAs in pneumonia diagnosis

Михайлова

Ivanoshchuk

Shakhtshneider

et al. 2020

SMJ

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Аннотация Пневмония-тяжелое воспалительное заболевание, характеризующееся высокой смертностью во всем мире. Ранний диагноз внегоспитальной пневмонии и выявление вызвавшего ее инфекционного агента могут существенно снизить тяжесть ее течения и вероятность госпитализации пациента. Используемые на данный момент диагностические и прогностические методы не являются достаточно надежными, поэтому ряд специфических некодирующих РНК, циркулирующих в крови в составе внеклеточных везикул, рассматривают в последние годы как потенциальный биомаркер для дифференциации вирусных и бактериальных пневмоний, а также для предсказания осложнений и тяжести течения заболевания. В обзоре рассмотрены микроРНК, длинные некодирующие РНК и ксено-микроРНК, которые были предложены в качестве прогностических и диагностических маркеров, а также потенциальных таргетных препаратов при пневмонии. Ключевые слова: пневмония, микроРНК, ксено-микроРНК, длинные некодирующие РНК, внеклеточные везикулы, биомаркер. Конфликт интересов: авторы заявляют об отсутствии конфликта интересов. Прозрачность финансовой деятельности: работа выполнена при частичной поддержке Комплексной программы фундаментальных исследований СО РАН АААА-А17-117112870181-6 и гранта РФФИ 17-04-02120.

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Section: микрорнкunclassified

Non-coding RNAs in pneumonia diagnosis

Михайлова

Ivanoshchuk

Shakhtshneider

et al. 2020

SMJ

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“…The inflammatory response of macrophages against L. pneumophila infection is mediated by miR-218 [ 103 ]. During L. pneumophila infection, the expression levels of miR-218 were upregulated and the expression levels of rapamycin-insensitive companion of mTOR (RICTOR) were downregulated.…”

Section: Introductionmentioning

confidence: 99%

The current landscape of microRNAs (miRNAs) in bacterial pneumonia: opportunities and challenges

2022

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MicroRNAs (miRNAs), which were initially discovered in Caenorhabditis elegans, can regulate gene expression by recognizing cognate sequences and interfering with the transcriptional or translational machinery. The application of bioinformatics tools for structural analysis and target prediction has largely driven the investigation of certain miRNAs. Notably, it has been found that certain miRNAs which are widely involved in the inflammatory response and immune regulation are closely associated with the occurrence, development, and outcome of bacterial pneumonia. It has been shown that certain miRNA techniques can be used to identify related targets and explore associated signal transduction pathways. This enhances the understanding of bacterial pneumonia, notably for “refractory” or drug-resistant bacterial pneumonia. Although these miRNA-based methods may provide a basis for the clinical diagnosis and treatment of this disease, they still face various challenges, such as low sensitivity, poor specificity, low silencing efficiency, off-target effects, and toxic reactions. The opportunities and challenges of these methods have been completely reviewed, notably in bacterial pneumonia. With the continuous improvement of the current technology, the miRNA-based methods may surmount the aforementioned limitations, providing promising support for the clinical diagnosis and treatment of “refractory” or drug-resistant bacterial pneumonia.

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“…Studies have shown that miRNAs are integral to the host response to bacterial, viral, and parasitic infections (24)(25)(26)(27)(28)(29)(30)(31); however, miRNAs could either promote or inhibit infection. For example, induction of miR-142-3p expression in macrophages leads to down-regulation of N-Wasp, an actin-binding protein, resulting in reduced uptake of Mycobacterium tuberculosis (32).…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs contribute to the host response to Coxiella burnetii

Sachan

Brann

Voth

et al. 2022

Preprint

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MicroRNAs (miRNAs), a class of small non-coding RNAs, are critical to gene regulation in eukaryotes. They are involved in modulating a variety of physiological processes, including the host response to intracellular infections. Little is known about miRNA functions during infection by Coxiella burnetii, the causative agent of human Q fever. This bacterial pathogen establishes a large replicative vacuole within macrophages by manipulating host processes such as apoptosis and autophagy. We investigated miRNA expression in C. burnetii-infected macrophages and identified several miRNAs that were down- or up-regulated during infection. We further explored the functions of miR-143-3p, an miRNA whose expression is down-regulated in macrophages infected with C. burnetii, and show that increasing the abundance of this miRNA in human cells results in increased apoptosis and reduced autophagy – conditions that are unfavorable to C. burnetii intracellular growth. In sum, this study demonstrates that C. burnetii infection elicits a robust miRNA-based host response, and because miR-143-3p promotes apoptosis and inhibits autophagy, down-regulation of miR-143-3p expression during C. burnetii infection likely benefits the pathogen.

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Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells

Cited by 5 publications

References 40 publications

Non-coding RNAs in pneumonia diagnosis

Non-coding RNAs in pneumonia diagnosis

The current landscape of microRNAs (miRNAs) in bacterial pneumonia: opportunities and challenges

MicroRNAs contribute to the host response to Coxiella burnetii

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