Legionella pneumonia as a cause of atypical pneumonia in a tertiary care hospital in Sri Lanka

Kottahachchi, J; Dissanayake, Dmbt; Athukorala, D.; Kottahachchi, S. P.; Fernando, S.S.N.; Weerasekara, M. M.; Gunasekara, T.D.C.P.; Nalaka, M. J.; Sudusinghe, Dinesha; Marasinghe, Chamil

doi:10.4038/ijms.v8i1.135

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…8 A study done by Kottahachchi et al in the Western Province of Sri Lanka also did not identify L. pneumophila in a sample of patients with atypical pneumonia. 9 According to the results of these studies L. pneumophila has been reported from the environment and humans. Since the first serologically confirmed case of L. pneumophila infection in 1993, no positive cases have been detected in the past three decades.…”

Section: Discussionmentioning

confidence: 99%

Molecular evidence of <em>Legionella pneumophila</em> associated lower respiratory tract infection in a COVID-19 suspected patient in Sri Lanka - A Case Report

Shiyamalee

Pattiyakumbura

Muthugala

et al. 2022

Sri Lankan J Infec Dis

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Legionella pneumophila is commonly found in aquatic environments. L. pneumophila is a causative agent of severe community acquired pneumonia and the infection is acquired through inhalation of aerosols containing the bacteria. In Sri Lanka, serologically confirmed cases of L. pneumophila infection in patients have been reported. However, there are no published reports on legionellosis in humans with evidence of polymerase chain reaction (PCR) based molecular confirmation in Sri Lanka. We report a PCR confirmed case of L. pneumophila associated lower respiratory tract infection in a 16-year-old monk who presented to a District General Hospital in the Central Province of Sri Lanka during the COVID-19 pandemic.

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Section: Discussionmentioning

confidence: 99%

Molecular evidence of <em>Legionella pneumophila</em> associated lower respiratory tract infection in a COVID-19 suspected patient in Sri Lanka - A Case Report

Shiyamalee

Pattiyakumbura

Muthugala

et al. 2022

Sri Lankan J Infec Dis

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Occurrence of the Legionella species in the respiratory samples of patients with pneumonia symptoms from Ahvaz, Iran; first detection of Legionella cherrii

et al. 2021

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Background This study aimed to investigate the occurrence of Legionella species in the respiratory samples of patients with pneumonia symptoms from Ahvaz, Iran by culture and the real-time PCR of 23S-5S rRNA gene spacer region. Methods and results A total of 123 clinical respiratory samples including 63 pleural aspirates, 57 bronchoalveolar lavage (BAL), and 3 sputum were collected from 65 males and 58 females with pneumonia symptoms. All samples were cultured on the Modified Wadowsky-Yee (MWY) agar. The Legionella species was identified by routine bacteriological tests. The presence of the 16S-23S rRNA spacer region gene was investigated by real-time PCR. The Legionella species were differentiated by sequencing of 16S-23S rRNA gene. A total of 2 (1.6%) BAL specimens were positive for Legionella species by culture method. No Legionella spp. were identified in pleural aspirates and sputum samples by the culture method. Using real-time PCR, 9 (7.3%) samples including 6 BAL, 1 sputum, and 2 pleural aspirates were positive for legionella species. These species were detected in 3 (5.2%) females and 6 males (9.2%). The results of sequencing showed that eight species were L. pneumophila while one was L. cherrii. Also, the 2 isolates that were identified by culture method, were confirmed as L. pneumophila by sequencing. Conclusions The results showed that using the real-time PCR has a more efficacy for detecting of Legionella species in respiratory samples. Also, L. pneumophila was the most prevalent species circulating in the southwest region of Iran. So, periodic monitoring programs is recommended to prevent epidemics due to this bacterium. KeywordsLegionella pneumophila • 16S-23S rRNA gene • Real-time PCR • Iran * Atosa Ghorbani

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Legionella pneumonia as a cause of atypical pneumonia in a tertiary care hospital in Sri Lanka

Cited by 2 publications

References 18 publications

Molecular evidence of <em>Legionella pneumophila</em> associated lower respiratory tract infection in a COVID-19 suspected patient in Sri Lanka - A Case Report

Molecular evidence of <em>Legionella pneumophila</em> associated lower respiratory tract infection in a COVID-19 suspected patient in Sri Lanka - A Case Report

Occurrence of the Legionella species in the respiratory samples of patients with pneumonia symptoms from Ahvaz, Iran; first detection of Legionella cherrii

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