Legionella jordanis isolated from a patient with fatal pneumonia

Thacker, W L; Wilkinson, Hazel W.; Benson, Robert F.; Edberg, Stephen C.; Brenner, Don J.

doi:10.1128/jcm.26.7.1400-1401.1988

Cited by 17 publications

(2 citation statements)

References 4 publications

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“…Legionella jordanis, first described in 1982 after recovery from the environment (1), has also been associated with fatal pneumonia (2) but remains a rare cause of infection. The Canadian National Microbiology Laboratory (NML) has been referred only two L.…”

Section: Announcementmentioning

confidence: 99%

Whole-Genome Sequencing of Legionella jordanis Strains NML 060502 and NML 130005, Recovered from a Lower Respiratory Tract Infection and Water, Respectively

Bernier

Bernard

2019

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

Whole-Genome Sequencing of Legionella jordanis Strains NML 060502 and NML 130005, Recovered from a Lower Respiratory Tract Infection and Water, Respectively

Bernier

Bernard

2019

Microbiol Resour Announc

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“…The genus Legionella currently contains 29 named species and multiple serogroups. Although Legionella pneumophila serogroup (SG) 1 is the most common causative agent of legionellosis (3,26), many different serogroups and species which cause disease in humans have been described (2,10,17,22,(30)(31)(32)(33)(34). The serodiagnosis of legionellosis is based mainly on the use of species-specific and serotype-specific monoclonal antibodies (MAbs) and antisera in indirect immunofluorescence.…”

mentioning

confidence: 99%

Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody

et al. 1991

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A monoclonal antibody (MAb) immunoglobulin G2a (2125) was produced against a 60-kDa Legionella heat shock protein (HSP), recognizing a unique epitope common to all species of the genus Legionella. The antibody reacted in the immunoblot with 59 Legionella species and serogroups that were tested and showed no cross-reactivity with other bacteria, including Acinetobacter spp., Bordetella spp., Pseudomonas spp., Mycobacterium spp., and Escherichia coli. Two other MAbs (2122 and 2130) reacted with the 60-kDa Legionella protein as well but showed different cross-reactivities with other gram-negative bacteria in the same molecular mass range. The genus-specific MAb 2125 as well as the cross-reacting MAbs 2122 and 2130 were shown to be reactive with the expressed protein of the cloned gene of the 60-kDa HSP of Legionella micdadei and Legionella pneumophila. These antibodies demonstrate that Legionella-specific and nonspecific epitopes are present on this protein. A sandwich enzyme-linked immunosorbent assay (ELISA) in which the genus-specific MAb is used both as a capture antibody and as a biotinylated second antibody has been established. With this test it is possible to detect Legionella whole cells, sonicated cells, and cell fractions containing the 60-kDa HSP. The main part of the 60-kDa HSP is found in the cytoplasmic fraction. The sandwich ELISA can be used to demonstrate the increased expression of the 60-kDa protein in Legionella cells following heat shock as well as marked differences in the detection of the 60-kDa HSP on whole cells of different Legionella strains. The high specificity and sensitivity of the sandwich ELISA for sonicated cells might be very useful to screen on a genus level for Legionella cells or the 60-kDa antigen in environmental isolates or body fluids of patients.

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Pneumonia

Seaton¹

2000

Crofton and Douglas's Respiratory Diseases

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Legionella jordanis isolated from a patient with fatal pneumonia

Cited by 17 publications

References 4 publications

Whole-Genome Sequencing of Legionella jordanis Strains NML 060502 and NML 130005, Recovered from a Lower Respiratory Tract Infection and Water, Respectively

Whole-Genome Sequencing of Legionella jordanis Strains NML 060502 and NML 130005, Recovered from a Lower Respiratory Tract Infection and Water, Respectively

Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody

Pneumonia

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