Left-handed helical structure of poly[d(A-C)].cntdot.poly[d(G-T)] studied by infrared spectroscopy

Taillandier, E.; Taboury, Jean; Adam, S.; Liquier, J.

doi:10.1021/bi00319a007

Cited by 58 publications

(27 citation statements)

References 20 publications

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“…The CD spectrum recorded in 5M NaCl, 95 mM NiCl 2 (Figure ld the Z conformation in consistence with previous I.R. results (14) and are now confirmed by recent Raman data (18) Our assignment is strongly supported by recently reported Raman spectra of poly d(A-T) · poly d(A-T) solutions in 5M NaCl, 95 mM NiC1 2 , which we also assigned to the Z conformation of the polynucleotide (20) in agreement with IR studies of poly d(A-T) · poly d(A-T) hydrated films in presence ofNiC1 2 (14). Among other characteristic features of the Z conformation, these spectra present a strong line located at 622 em-', reflecting the C3'-endo/syn geometry of the adenosines.…”

Section: Resultssupporting

confidence: 88%

“…Therefore the anti-syn reorientation of adenosines is characterized in CD spectra in a similar way as that of guanosines. The same similitude of spectral changes for these three polynucleotides has been also observed in vibrational spectra (11,14,15,18,20). In the same way, the crystal structures of oligonucleotides containing A-T base pairs between numerous G-C ones exhibit an overall form very similar to that of d(G-C) 3 (12,31 ,32).…”

Section: Thecdspectrumofpolyd(a-t) · Polyd(a-t)in50mmnacl(supporting

confidence: 67%

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Z Form of Poly d(A-T) · Poly d(A-T) in Solution Studied by CD and UV Spectroscopies

Bourtayre

Liquier

Pizzorni

et al. 1987

Journal of Biomolecular Structure and Dynamics

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Near UV CD spectra, UV absorption spectra and their first derivatives have been recorded on poly d(A-T).poly d(A-T) solutions in presence of high NaCl concentration and various amounts of NiCl2. Comparison of the results presented here with those obtained for poly d(G-C).poly d(G-C) and poly d(A-C).poly d(G-T) in comparable conditions, and the I.R. and Raman data on poly d(A-T).poly d(A-T), allows us to assign the new spectra to the Z conformation of poly d(A-T).poly d(A-T) in solution. The mechanism by which nickel ions induce the B----Z interconversion in the presence of high NaCl concentration is discussed.

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Section: Resultssupporting

confidence: 88%

Section: Thecdspectrumofpolyd(a-t) · Polyd(a-t)in50mmnacl(supporting

confidence: 67%

Z Form of Poly d(A-T) · Poly d(A-T) in Solution Studied by CD and UV Spectroscopies

Bourtayre

Liquier

Pizzorni

et al. 1987

Journal of Biomolecular Structure and Dynamics

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“…Figure 3 shows the micro-FTIR spectra of the same samples as in A set of ir Z-form marker bands has been previously obtained and used to detect the Z geometry in poly-and oligodeoxynucleo-( Table I ) . The presence of these marker bands on the FTIR spectrum of the ortides 18,20,[36][37][38] thorhombic d ( C2GCG2) crystal clearly shows that it adopts a Z conformation.…”

Section: The Z Conformation Of D(c2gcc2)mentioning

confidence: 99%

A and Z canonical conformations in d(C_nGCG_n) crystals characterized by microFTIR and microRaman spectroscopies

et al. 1993

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Two crystals d(C2GCG2) and d(C5GCG5) have been studied under microscope by Fourier transform ir spectroscopy and Raman spectroscopy. The x-ray diffraction study of the latter crystal had shown that the d(C5GCG5) sequence is the first DNA dodecamer known to adopt a canonical A conformation [N. Verdaguer, J. Aymami, D. Fernandez-Forner, I. Fita, M. Coll, T. Huynh-Dinh, J. Igolen, and J. A. Subirana (1991) Journal of Molecular Biology, Vol. 221, pp. 623-635]. Characteristic ir marker bands and Raman marker peaks of the A conformation have thus been obtained and are compared with previously proposed assignments correlated to fiber diffraction x-ray results obtained on polymers. The d(C2GCG2) sequence crystal had previously been studied in an intermediate form between B and Z [L. Urpi, J. P. Ridoux, J. Liquier, N. Verdagner, I. Fita, J. A. Subirana, F. Iglesias, T. Huynh-Dinh, J. Igolen, and E. Taillandier (1989) Nucleic Acids Research, Vol. 17, pp. 6669-6679]. In this paper we present results obtained from a crystal with this oligonucleotide in Z conformation. The effect of the crystallization conditions on the geometry of the obtained oligomer helix is discussed. The influence of the addition, to the central tetramer CGCG, of dCn stretches (at the 5' end) and dGn stretches (at the 3' end) of different lengths, on the conformational flexibility of the nucleic acid, is considered.

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“…In the case of methylated hexamer, the formation of the Z double helix which is dependent on salt concentration was found by 1 H-NMR to take place via formation of a B double helix even in high salt solution (4 M NaCl) (10,11). On the other hand, the IR spectroscopy on condensed phase has shown the existence of Z form for poly(dA-dC)• poly(dG-dT) (14) as well as for d(C-2amino A-C-G-T-G) and d(m 5 C-G-C-A-m 5 C-G-T-G-C-G) whereas in solution only a fraction of the two latter ones undergoes the B-Z transition (13).…”

mentioning

confidence: 99%

Z Helix-coil Transition of d(C-Br⁸G-C-G-C-Br⁸G) Studied by CD,¹H-NMR and IR Spectroscopies

Taboury

Taillandier

Lumbroso

et al. 1985

Journal of Biomolecular Structure and Dynamics

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The conformation of d(C-Br8G-C-G-C-Br8G) in aqueous solution was studied by CD and 1H-NMR spectroscopy and in condensed phase by IR spectroscopy. Whether in 0.1 M or 3 M NaCl solution or in film the only double helical structure adopted by brominated d(C-G)3 oligomer is the Z form. The IR spectrum of the film presents all the characteristic absorptions of the Z conformation and in particular is indicative of a syn conformation for the central guanosine as well as for the brominated one. Imino proton resonances of d(C-Br8G-C-G-C-Br8G) demonstrating the duplex formation were observed up to 60 degrees C. It is interesting to note that the significant highfield shifts of the dC H5" exocyclic sugar protons characteristic of the non exchangeable proton spectra of d(C-G)3 containing 5-methyl dC residues in the Z form were also detected in the proton spectrum of brominated oligomer. Whereas formation of the Z helix of methylated d(C-G)3 oligomers dependent on the salt concentration was found to occur via the preliminary formation of a B helix even in 4 M NaCl solution, the Z helix of d(C-Br8G-C-G-C-Br8G) is obtained directly from the coil form. However, IR data suggest that in the Z form of d(C-Br8G-C-G-C-Br8G), the overlapping of the base planes should be slightly different in comparison with the stacking observed in d(C-G)3 crystals. The kinetic data (activation energy and lifetime) of the Z helix-coil transition of brominated d(C-G)3 are compared to those of the B helix-coil transition observed for methylated d(C-G)3 in 0.1 M NaCl solution while the thermodynamic data of these two reactions (enthalpy and midpoint temperature) are slightly different.

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Left-handed helical structure of poly[d(A-C)].cntdot.poly[d(G-T)] studied by infrared spectroscopy

Cited by 58 publications

References 20 publications

Z Form of Poly d(A-T) · Poly d(A-T) in Solution Studied by CD and UV Spectroscopies

Z Form of Poly d(A-T) · Poly d(A-T) in Solution Studied by CD and UV Spectroscopies

A and Z canonical conformations in d(C_nGCG_n) crystals characterized by microFTIR and microRaman spectroscopies

Z Helix-coil Transition of d(C-Br⁸G-C-G-C-Br⁸G) Studied by CD,¹H-NMR and IR Spectroscopies

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Left-handed helical structure of poly[d(A-C)].cntdot.poly[d(G-T)] studied by infrared spectroscopy

Cited by 58 publications

References 20 publications

Z Form of Poly d(A-T) · Poly d(A-T) in Solution Studied by CD and UV Spectroscopies

Z Form of Poly d(A-T) · Poly d(A-T) in Solution Studied by CD and UV Spectroscopies

A and Z canonical conformations in d(CnGCGn) crystals characterized by microFTIR and microRaman spectroscopies

Z Helix-coil Transition of d(C-Br8G-C-G-C-Br8G) Studied by CD,1H-NMR and IR Spectroscopies

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A and Z canonical conformations in d(C_nGCG_n) crystals characterized by microFTIR and microRaman spectroscopies

Z Helix-coil Transition of d(C-Br⁸G-C-G-C-Br⁸G) Studied by CD,¹H-NMR and IR Spectroscopies