LEF/TCF: Its Role in Colon Cancer

Bastien, Amanda J.

doi:10.1007/978-981-10-6728-0_22

Cited by 1 publication

(1 citation statement)

References 66 publications

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“…Wnt signaling is particularly relevant in colorectal cancer, since the vast majority of colorectal cancers harbor Wnt/β-catenin pathway-activating mutations [15], which contrasts with a normal benevolent role in regulating stem-cell-mediated maintenance of intestinal and colorectal epithelial tissue [16,17]. Roles for LEF/TCF proteins has been studied in tissue-culture and animal models of intestinal stem cells and colorectal cancer, which suggested roles for TCF7L2 and TCF7 in normal colorectal tissue, where LEF1 and TCF7L1 are silent, but strong expression in colorectal tumor of LEF1 [9,[18][19][20][21] and TCF7 [22,23].…”

Section: Introductionmentioning

confidence: 99%

Diverse LEF/TCF Expression in Human Colorectal Cancer Correlates with Altered Wnt-Regulated Transcriptome in a Meta-Analysis of Patient Biopsies

Mayer

Giclais

Alsehly

et al. 2020

Genes

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Aberrantly activated Wnt signaling causes cellular transformation that can lead to human colorectal cancer. Wnt signaling is mediated by Lymphoid Enhancer Factor/T-Cell Factor (LEF/TCF) DNA-binding factors. Here we investigate whether altered LEF/TCF expression is conserved in human colorectal tumor sample and may potentially be correlated with indicators of cancer progression. We carried out a meta-analysis of carefully selected publicly available gene expression data sets with paired tumor biopsy and adjacent matched normal tissues from colorectal cancer patients. Our meta-analysis confirms that among the four human LEF/TCF genes, LEF1 and TCF7 are preferentially expressed in tumor biopsies, while TCF7L2 and TCF7L1 in normal control tissue. We also confirm positive correlation of LEF1 and TCF7 expression with hallmarks of active Wnt signaling (i.e., AXIN2 and LGR5). We are able to correlate differential LEF/TCF gene expression with distinct transcriptomes associated with cell adhesion, extracellular matrix organization, and Wnt receptor feedback regulation. We demonstrate here in human colorectal tumor sample correlation of altered LEF/TCF gene expression with quantitatively and qualitatively different transcriptomes, suggesting LEF/TCF-specific transcriptional regulation of Wnt target genes relevant for cancer progression and survival. This bioinformatics analysis provides a foundation for future more detailed, functional, and molecular analyses aimed at dissecting such functional differences.

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