LED-based photoacoustic imaging for early detection of joint inflammation in rodents: towards achieving 3Rs in rheumatoid arthritis research

Joseph, Francis Kalloor; Xavierselvan, Marvin; Singh, Mithun Kuniyil Ajith; Mallidi, Srivalleesha; Laken, Conny van der; Loo, Faj van de; Steenbergen, Wiendelt

doi:10.1117/12.2545915

Cited by 5 publications

(5 citation statements)

References 12 publications

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“…The ability to accurately estimate the oxygen concentration makes multiwavelength PAI a potential tool for early detection of RA. Joseph et al used in vivo animal study to find the capability of measuring the oxygen saturation using this system [77]. First, ex-vivo PA oxygen saturation imaging using human blood was validated against oximeter readings and further verified with in-vivo animal studies.…”

Section: Oxygen Saturation Imaging In Rheumatoid Arthritis Diagnosismentioning

confidence: 99%

Towards Clinical Translation of LED-Based Photoacoustic Imaging: A Review

Zhu

Tian

Cheng

et al. 2020

Sensors

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Photoacoustic imaging, with the capability to provide simultaneous structural, functional, and molecular information, is one of the fastest growing biomedical imaging modalities of recent times. As a hybrid modality, it not only provides greater penetration depth than the purely optical imaging techniques, but also provides optical contrast of molecular components in the living tissue. Conventionally, photoacoustic imaging systems utilize bulky and expensive class IV lasers, which is one of the key factors hindering the clinical translation of this promising modality. Use of LEDs which are portable and affordable offers a unique opportunity to accelerate the clinical translation of photoacoustics. In this paper, we first review the development history of LED as an illumination source in biomedical photoacoustic imaging. Key developments in this area, from point-source measurements to development of high-power LED arrays, are briefly discussed. Finally, we thoroughly review multiple phantom, ex-vivo, animal in-vivo, human in-vivo, and clinical pilot studies and demonstrate the unprecedented preclinical and clinical potential of LED-based photoacoustic imaging.

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Section: Oxygen Saturation Imaging In Rheumatoid Arthritis Diagnosismentioning

confidence: 99%

Towards Clinical Translation of LED-Based Photoacoustic Imaging: A Review

Zhu

Tian

Cheng

et al. 2020

Sensors

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“…For all the phantom and human volunteer imaging experiments, we used the commercially available LED-based PA and US imaging system (AcousticX, CYBERDYNE INC, Japan) (Fig. 1a) [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] . AcousticX is the only plug-and-play PA and US imaging system commercially available in the market now.…”

Section: Led-based Pa and Us Imaging System (Acousticx)mentioning

confidence: 99%

“…9 We recently developed and commercialized an LED-based PA and US imaging system (AcousticX) and have already demonstrated its potential in multiple preclinical applications and early clinical pilot studies. [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] In AcousticX, clinical linear array US probes and high-power LED arrays are used in a handheld probe to perform real-time 2D US, PA, and oxygen saturation imaging. 10 In our previous work, we implemented automatic linear scanning of the AcousticX PA/US probe to generate 3D PA and US MIP images of the human vasculature.…”

Section: Introductionmentioning

confidence: 99%

Improving 3D imaging capability of LED-based photoacoustic imaging for high resolution human vascular imaging applications

Singh¹,

Sato²,

Ichihashi³

et al. 2023

Photons Plus Ultrasound: Imaging and Sensing 2023

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Visualizing blood vessel networks in 3D is helpful in many superficial vascular imaging applications. We previously reported the feasibility of LED-based photoacoustic imaging in visualizing human vasculature in 3D by linear translation of combined photoacoustic/ultrasound imaging probe. In this work, we improved this 3D imaging functionality by 1) automatic removal of skin PA signal in different 2D slices by ultrasound-based lineation , 2) encoding depth information with different colors and 3)improving the grayscale and hot color maps. All these new features were implemented in the system software and we validated these using phantom and human in vivo imaging experiments. Results demonstrate that the newly implemented features significantly improved the 3D imaging capability in our LED-based photoacoustic and ultrasound imaging system. We believe that the improved 3D imaging functionality in the system will be potentially useful for multiple preclinical and clinical applications in which the visualization of vasculature in 3D is a prerequisite.

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“…Addressing this hurdle, we introduced and commercialized an LED-based PA and US imaging system, known as AcousticX. This alternative to laser-based equipment has demonstrated significant potential in various preclinical applications and early clinical pilot studies [9][10][11][12][13][14][15][16][17][18][19][20][21][22] . In this work, we demonstrate the potential of LED-based photoacoustic imaging for microvascular health assessment through in vivo human volunteer imaging We used AcousticX to image micro-vasculature in foot of a human volunteer in 3D.…”

Section: Introductionmentioning

confidence: 99%

LED-based photoacoustic imaging: a point-of-care solution for microvascular health assessment

Kuniyil Ajith Singh,

Sato,

Ichihashi

et al. 2024

Photons Plus Ultrasound: Imaging and Sensing 2024

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Accurate assessment of microvasculature and oxygen saturation is vital for diagnosing and monitoring diseases, including cancer. However, the current clinical need for point-of-care (POC), non-invasive, and cost-effective imaging approaches remains unmet. Photoacoustic imaging, offering natural blood contrast, holds potential for high-resolution vascular imaging. Yet, the requirement for bulky and expensive lasers impedes its clinical translation, particularly in resource-limited settings. Recent advances in using high-power LED arrays for photoacoustic imaging are promising due to their portability, affordability, and ease-of-use in clinical settings. In this work, we demonstrate the potential of LED-based photoacoustic imaging for microvascular health assessment through in vivo human volunteer imaging. Real-time 2D and 3D experiments evaluated LED-based photoacoustic imaging in assessing vascular density, arterial distensibility, and blood oxygen saturation with high resolution. Our results confirm that LED-based photoacoustic imaging may serve as an invaluable POC tool for microvascular health assessment in resource-limited settings. The affordability and simplicity of LED arrays present a compelling alternative to laser-based approaches, expanding accessibility in clinical practice. This advancement has the potential to enhance early disease detection and treatment monitoring, particularly in areas with limited access to sophisticated imaging technologies.

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LED-based photoacoustic imaging for early detection of joint inflammation in rodents: towards achieving 3Rs in rheumatoid arthritis research

Abstract: van der Laken, Fons van de Loo, Wiendelt Steenbergen, "LED-based photoacoustic imaging for early detection of joint inflammation in rodents: towards achieving 3Rs in rheumatoid arthritis research,"

Cited by 5 publications

References 12 publications

Towards Clinical Translation of LED-Based Photoacoustic Imaging: A Review

Towards Clinical Translation of LED-Based Photoacoustic Imaging: A Review

Improving 3D imaging capability of LED-based photoacoustic imaging for high resolution human vascular imaging applications

LED-based photoacoustic imaging: a point-of-care solution for microvascular health assessment

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