Lectins of Distinct Specificity in
            <i>Rhodnius prolixus</i>
            Interact Selectively with
            <i>Trypanosoma cruzi</i>

Pereira, M. E. A.; Andrade, Arnaldo F.B.; Ribeiro, José M. C.

doi:10.1126/science.7006082

Cited by 154 publications

(57 citation statements)

References 9 publications

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“…It is possible that the interaction of cell-surface glycoproteins with lectins, which have been described in the gut of the insect vector (Pereira et al, 1981), might play a role in the morphogenesis of T. cruzi. Clearly, further biochemical characterization of the glycoconjugates on the surface of T. cruzi will be needed to help clarify their possible role during differentiation of the parasite.…”

Section: Discussionmentioning

confidence: 99%

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Andrade¹,

Esteves²,

Angluster³

et al. 1991

Journal of General Microbiology

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Highly purified lectins with specificities for receptor molecules containing sialic acid, N-acetylglucosamine (DGlcNAc), N-acetylgalactosamine (D-GalNAc), galactose (D-Gal), mannose-like residues (D-Man) or L-fucose (LFuc), were used to determine changes in cell-surface carbohydrates of the protozoal parasite Trypanosomu cruzi during metacyclogenesis under chemically defined conditions. Of the D-GalNAc-binding lectins, BS-I selectively agglutinated metacyclic trypomastigotes, MPL was selective for replicating epimastigotes, whereas SBA strongly agglutinated all developmental stages of T. cruzi. WGA (sialic acid and/or D-GIcNAc specific) was also reactive with differentiating epimastigotes and metacyclic trypomastigotes but displayed a higher reactivity with replicating epimastigote forms. A progressive decrease in agglutinating activity was observed for jacaline (specific for D-Gal) during the metacyclogenesis process; conversely, a progressive increase in affinity was observed for RCA-I (D-Gabspecific), although the reactivity of other D-Gal-specific lectins (PNA and AxP) was strong at all developmental stages. All developmental stages of T. cruzi were agglutinated by Con A and Lens cufinaris lectins (specific for D-Man-like residues); however, they were unreactive with the L-fucose-binding lectins from Lotus tetrugonolobos and Ufex europueus. These agglutination assays were further confirmed by binding studies using I z51-labelled lectins. Neuraminidase activity was detected in supernatants of cell-free differentiation medium using the PNA hemagglutination test with human A erythrocytes. The most pronounced differences in lectin agglutination activity were observed between replicating and differentiating epimastigotes, suggesting that changes in the composition of accessible cell-surface carbohydrates precede the morphological transformation of epimastigotes into metacyclic trypomastigotes.

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Section: Discussionmentioning

confidence: 99%

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Andrade¹,

Esteves²,

Angluster³

et al. 1991

Journal of General Microbiology

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“…Blood-sucking arthropods provide a good opportunity to investigate and discuss structural and functional aspects of the lectins and protein-carbohydrate inter actions taking place in the mechanisms of pathogen/ parasite transmission (for review Ingram & Molyneux, 1991). The first milestone paper on tissue specific lec tins of a vector was published on the kissing bug Rhodnius prolixus by Pereira et al (1981). However, most results on vector lectins have come from research on tsetse flies and have shown that tissue specific lectins might play the crucial role in control of tsetse fly infection by African trypanosomes (for review Maudlin & Welburne, 1988;Maudlin, 1991).…”

Section: Lectins In Arthropods and Vector-pathogen Systemsmentioning

confidence: 99%

“…Although many triatomine species are capable of the disease transmission, most of the knowledge on the vectorparasite interactions and the possible role of lectins and lectin-like activities has been derived from few model species, mostly Rhodnius prolixus and Triatoma infestans. In the course of these studies, several hemagglutin activities have been detected in various tissues such as hemolymph, salivary glands, crop and midgut (Pereira et al, 1981 ;Barraco & Loch, 1988;Gomes et al, 1988;Gomes et al, 1991 ;Gregorio & Ratcliffe, 1991 ;Hypsa & Grubhoffer, 1995) and the possible significance of these compounds for T. cruzi deve lopment has been suggested. Since T. cruzi does not invade the host hemocoel and its complete develop ment takes place in gut lumen, the gut agglutinins may be of particular importance in respect of the deve lopment of this parasite.…”

Section: Lectins In Arthropods and Vector-pathogen Systemsmentioning

confidence: 99%

“…The investigation of triatomine tissue lectins and their role in host-parasite interactions began with the clas sical study by Pereira and co-workers (Pereira et al, 1981) who observed a hemagglutination activity against rabbit erythrocytes in hemolymph, midgut and crop of Rhodnius prolixus. They demonstrated that the agglu tinins from individual tissues represented distinct lec tins differring in their binding specificities.…”

Section: Lectins In Arthropods and Vector-pathogen Systemsmentioning

confidence: 99%

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Lectins (hemagglutinins) in the gut of the important disease vectors

1997

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Summary :The review is devoted to the gut lectins/hemagglutinins of the following representatives of important disease vectors : ticks, kissing-bugs, mosquitoes, sandflies and tsetse flies. The paper surveys the recent knowledge on these carbohydrate binding factors with respect to their structural and functional properties, and their significance for pathogen/parasite transmission by the blood-sucking arthropods. Recent results suggest that in most vectors the gut lectin activities are blood-meal enhanced, might participate in blood-meal processing and digestion and could serve as antibacterial and antiparasitic agents. Recently, protein-protein interactions have been studied in mechanisms of cell adhesion (integrins vs adhesins), and so-called scavenger proteins have been described as representatives of the protein-lipid/phospholipid interactions (for review Pearson, 1996

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“…In this context, they are submitted to drastic changes in temperature, pH and osmolarity and are exposed to saliva, gut contents and blood digestion products [12]. Some factors involved in the development of T. cruzi in the gut of the vector have been identified, including a lytic factor [19,20], lectins [21,22] and hemoglobin constituents [23]. Other factors are suggested to trigger T. cruzi metacyclogenesis in vitro, including cAMP, cAMP analogues and adenylyl cyclase activators.…”

Section: Introductionmentioning

confidence: 99%

Cell Signaling During Trypanosoma cruzi Development in Triatominae

Silva-Neto¹

2010

TOPARAJ

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Abstract:The year 2009 is the centennial anniversary of the original description of Chagas disease by Carlos Chagas. During the last 100 years, several advances have occurred regarding our knowledge of the development of Trypanosoma cruzi and its travel along the gut of Triatominae vectors. We have also witnessed the completion of both the human and parasite genome projects; the genome of one of Chagas disease vectors, Rhodnius prolixus, is currently being sequenced. The development of T. cruzi in triatomine gut relies on several biochemical and molecular processes. The biochemistry of blood digestion and the molecular and biological aspects of parasite development are well known. However, several signaling molecules are generated during blood digestion, and their effects on parasite biology are only beginning to be understood. Here, we will summarize our current knowledge in this area with an emphasis on heme and bioactive lipids. In addition, we will highlight some recently described members of the parasite signaling machinery, which were identified through high-throughput studies, but whose ligands are unknown thus far. Finally, we will discuss some potential aspects for future investigation in this area that may strengthen our view of such a concomitant biological process in the next years.

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Lectins of Distinct Specificity in Rhodnius prolixus Interact Selectively with Trypanosoma cruzi

Cited by 154 publications

References 9 publications

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Lectins (hemagglutinins) in the gut of the important disease vectors

Cell Signaling During Trypanosoma cruzi Development in Triatominae

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