Lectins Homologous to Those of Monocotyledonous Plants in the Skin Mucus and Intestine of Pufferfish, Fugu rubripes

Tsutsui, Shigeyuki; Tasumi, Satoshi; Suetake, Hiroaki; Suzuki, Yuzuru

doi:10.1074/jbc.m301038200

Cited by 101 publications

(62 citation statements)

References 41 publications

(45 reference statements)

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“…Taken together, these results demonstrate that skin mucus lectins in fishes have wide molecular diversity [200]. In another study, a piscine lily-type lectin was described in pufferfish and it was expressed exclusively in mucosal tissues, namely, skin, digestive tract, oral cavity, and gills [201].…”

Section: Lectinsmentioning

confidence: 85%

An Overview of the Immunological Defenses in Fish Skin

2012

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The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal's immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

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Section: Lectinsmentioning

confidence: 85%

An Overview of the Immunological Defenses in Fish Skin

2012

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“…More specifically, changes in the composition of mucus in parasitized fish could be related to synthesis of molecules involved in the mechanism of 'exclusion' of the parasites from their preferred site in the gut (Miller 1987(Miller , 1996. For example, pufflectin is the first fish mucosal glycoconjugate produced in gills, skin and intestine of Fugu rubripes that has recently been demonstrated to bind to parasitic metazoans (Tsutsui et al 2003). The thickness of adherent mucous gel is significantly higher in the proximal intestine of Salmo trutta infected with helminths than in the uninfected brown trout.…”

Section: Discussionmentioning

confidence: 99%

Histochemistry of glycoconjugates in mucous cells of Salmo trutta uninfected and naturally parasitized with intestinal helminths

Bosi¹,

Arrighi²,

Giancamillo³

et al. 2005

Dis. Aquat. Org.

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Mucus secreted onto the surface of the intestine forms a physical barrier to invading parasites so that a possible attachment of helminths to the surface is prevented and their expulsion by peristalsis facilitated. In mammals, intestinal parasites induce hyperplasia and hypertrophy of intestine goblet cells and provoke changes in the mucus composition. In fish, this topic has received less attention. In the present investigation, histochemical methods were employed to compose intestinal mucous cell numbers and their glycoconjugate composition were compared by uninfected brown trout Salmo trutta and in S. trutta parasitized with Cyathocephalus truncatus or Pomphorhynchus laevis. When P. laevis was present in the intestine of the brown trout, the total mucous cell number, and the number of mucous cells containing acid or mixed glycoconjugates were significantly enhanced. No significant change in the total mucous cell number was detected in the intestine of fish parasitized with C. truncatus in comparison with uninfected brown trout. A significant increase was observed in the number of both acid (especially sulphated) and mixed glycoconjugates containing mucous cells as well as a significant decrease in the number of neutral glycoconjugates containing mucous cells. When intestinal helminths were present, the thickness of the adherent mucous gel increased. In a limited number of other fish species, the occurrence of gill and intestinal parasites has been reported to increase the mucosal glycoconjugate secretions. Our study is the first quantitative report on the effects of intestinal helminths on the density of mucous cells and mucus composition in a fish species. KEY WORDS: Glycoconjugates · Mucus composition · Mucous cell number · Intestinal parasites · Brown troutResale or republication not permitted without written consent of the publisher

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“…Four micrograms of poly(A) ϩ RNA from each tissue was separated on a 1.5% formamide-agarose denaturing gel. Northern blot analysis was then performed according to the method of Tsutsui et al (36). The probe used was a partial cDNA encoding fugu pIgR Ig-like domains.…”

Section: Northern Blot Analysismentioning

confidence: 99%

A Teleost Polymeric Ig Receptor Exhibiting Two Ig-Like Domains Transports Tetrameric IgM into the Skin

Hamuro

Suetake

Saha³

et al. 2007

The Journal of Immunology

Self Cite

129

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The skin mucus IgM is an important molecule in the mucosal immune system of teleost skin. However, the transport mechanism associated with this molecule has yet to be clarified. In this study, we isolated a gene encoding a polymeric Ig receptor (pIgR) from a species of teleost fish, Takifugu rubripes (fugu). This gene is known to be an Ig transporter in the intestine of mammals. Our studies further demonstrated that fugu pIgR was expressed in the skin and that a fragment of pIgR bound to tetrameric IgM in the skin mucus. These results indicate that the skin pIgR transports tetrameric IgM into the skin mucus. The fugu pIgR exhibits a unique structure containing only two Ig-like domains corresponding to domain 1 and domain 4/5 of mammalian pIgR. This structure was sufficient for successful binding to tetrameric IgM. Teleost skin thus adopts the same Ig transport system as mammalian intestine via a unique pIgR.

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Lectins Homologous to Those of Monocotyledonous Plants in the Skin Mucus and Intestine of Pufferfish, Fugu rubripes

Cited by 101 publications

References 41 publications

An Overview of the Immunological Defenses in Fish Skin

An Overview of the Immunological Defenses in Fish Skin

Histochemistry of glycoconjugates in mucous cells of Salmo trutta uninfected and naturally parasitized with intestinal helminths

A Teleost Polymeric Ig Receptor Exhibiting Two Ig-Like Domains Transports Tetrameric IgM into the Skin

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